A self-labeling protein based on the small ultra-red fluorescent protein, smURFP

Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches...

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Detalles Bibliográficos
Autores principales: Machado, John-Hanson, Ting, Richard, Lin, John Y., Rodriguez, Erik A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341759/
https://www.ncbi.nlm.nih.gov/pubmed/34458834
http://dx.doi.org/10.1039/d1cb00127b
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author Machado, John-Hanson
Ting, Richard
Lin, John Y.
Rodriguez, Erik A.
author_facet Machado, John-Hanson
Ting, Richard
Lin, John Y.
Rodriguez, Erik A.
author_sort Machado, John-Hanson
collection PubMed
description Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development.
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spelling pubmed-83417592021-08-26 A self-labeling protein based on the small ultra-red fluorescent protein, smURFP Machado, John-Hanson Ting, Richard Lin, John Y. Rodriguez, Erik A. RSC Chem Biol Chemistry Self-labeling proteins have revolutionized super-resolution and sensor imaging. Tags recognize a bioorthogonal substrate for covalent attachment. We show the small Ultra-Red Fluorescent Protein (smURFP) is a self-labeling protein. The substrate is fluorogenic, fluoresces when attached, and quenches fluorescent cargo. The smURFP-tag has novel properties for tool development. RSC 2021-06-29 /pmc/articles/PMC8341759/ /pubmed/34458834 http://dx.doi.org/10.1039/d1cb00127b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Machado, John-Hanson
Ting, Richard
Lin, John Y.
Rodriguez, Erik A.
A self-labeling protein based on the small ultra-red fluorescent protein, smURFP
title A self-labeling protein based on the small ultra-red fluorescent protein, smURFP
title_full A self-labeling protein based on the small ultra-red fluorescent protein, smURFP
title_fullStr A self-labeling protein based on the small ultra-red fluorescent protein, smURFP
title_full_unstemmed A self-labeling protein based on the small ultra-red fluorescent protein, smURFP
title_short A self-labeling protein based on the small ultra-red fluorescent protein, smURFP
title_sort self-labeling protein based on the small ultra-red fluorescent protein, smurfp
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341759/
https://www.ncbi.nlm.nih.gov/pubmed/34458834
http://dx.doi.org/10.1039/d1cb00127b
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