One-pot fluorescent assay for sensitive detection of APOBEC3A activity

We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and scr...

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Detalles Bibliográficos
Autores principales: Wang, Bingyao, Zhang, Xiong, Wang, Yafen, Chen, Kun, Wang, Fang, Weng, Xiaocheng, Zhou, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341802/
https://www.ncbi.nlm.nih.gov/pubmed/34458832
http://dx.doi.org/10.1039/d1cb00076d
Descripción
Sumario:We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.

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