Cargando…

Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients

INTRODUCTION: The Cancer Genome Atlas (TCGA) project and Asian Cancer Research Group (ACRG) recently categorized gastric cancer into molecular subtypes. Nevertheless, these classification systems require high cost and sophisticated molecular technologies, preventing their widespread use in the clini...

Descripción completa

Detalles Bibliográficos
Autores principales: Nshizirungu, Jean Paul, Bennis, Sanae, Mellouki, Ihsane, Sekal, Mohammed, Benajah, Dafr-Allah, Lahmidani, Nada, El Bouhaddouti, Hicham, Ibn Majdoub, Karim, Ibrahimi, Sidi Adil, Celeiro, Sónia Pires, Viana-Pereira, Marta, Munari, Fernanda Franco, Ribeiro, Guilherme Gomes, Duval, Vinicius, Santana, Iara, Reis, Rui Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342151/
https://www.ncbi.nlm.nih.gov/pubmed/34367379
http://dx.doi.org/10.1155/2021/9980410
_version_ 1783734006142992384
author Nshizirungu, Jean Paul
Bennis, Sanae
Mellouki, Ihsane
Sekal, Mohammed
Benajah, Dafr-Allah
Lahmidani, Nada
El Bouhaddouti, Hicham
Ibn Majdoub, Karim
Ibrahimi, Sidi Adil
Celeiro, Sónia Pires
Viana-Pereira, Marta
Munari, Fernanda Franco
Ribeiro, Guilherme Gomes
Duval, Vinicius
Santana, Iara
Reis, Rui Manuel
author_facet Nshizirungu, Jean Paul
Bennis, Sanae
Mellouki, Ihsane
Sekal, Mohammed
Benajah, Dafr-Allah
Lahmidani, Nada
El Bouhaddouti, Hicham
Ibn Majdoub, Karim
Ibrahimi, Sidi Adil
Celeiro, Sónia Pires
Viana-Pereira, Marta
Munari, Fernanda Franco
Ribeiro, Guilherme Gomes
Duval, Vinicius
Santana, Iara
Reis, Rui Manuel
author_sort Nshizirungu, Jean Paul
collection PubMed
description INTRODUCTION: The Cancer Genome Atlas (TCGA) project and Asian Cancer Research Group (ACRG) recently categorized gastric cancer into molecular subtypes. Nevertheless, these classification systems require high cost and sophisticated molecular technologies, preventing their widespread use in the clinic. This study is aimed to generating molecular subtypes of gastric cancer using techniques available in routine diagnostic practice in a series of Moroccan gastric cancer patients. In addition, we assessed the associations between molecular subtypes, clinicopathological features, and prognosis. METHODS: Ninety-seven gastric cancer cases were classified according to TCGA, ACRG, and integrated classifications using a panel of four molecular markers (EBV, MSI, E-cadherin, and p53). HER2 status and PD-L1 expression were also evaluated. These markers were analyzed using immunohistochemistry (E-cadherin, p53, HER2, and PD-L1), in situ hybridization (EBV and HER2 equivocal cases), and multiplex PCR (MSI). RESULTS: Our results showed that the subtypes presented distinct clinicopathological features and prognosis. EBV-positive gastric cancers were found exclusively in male patients. The GS (TCGA classification), MSS/EMT (ACRG classification), and E-cadherin aberrant subtype (integrated classification) presented the Lauren diffuse histology enrichment and tended to be diagnosed at a younger age. The MSI subtype was associated with a better overall survival across all classifications (TCGA, ACRG, and integrated classification). The worst prognosis was observed in the EBV subtype (TCGA and integrated classification) and MSS/EMT subtype (ACRG classification). Discussion/Conclusion. We reported a reproducible and affordable gastric cancer subtyping algorithms that can reproduce the recently recognized TCGA, ACRG, and integrated gastric cancer classifications, using techniques available in routine diagnosis. These simplified classifications can be employed not only for molecular classification but also in predicting the prognosis of gastric cancer patients.
format Online
Article
Text
id pubmed-8342151
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-83421512021-08-06 Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients Nshizirungu, Jean Paul Bennis, Sanae Mellouki, Ihsane Sekal, Mohammed Benajah, Dafr-Allah Lahmidani, Nada El Bouhaddouti, Hicham Ibn Majdoub, Karim Ibrahimi, Sidi Adil Celeiro, Sónia Pires Viana-Pereira, Marta Munari, Fernanda Franco Ribeiro, Guilherme Gomes Duval, Vinicius Santana, Iara Reis, Rui Manuel Dis Markers Research Article INTRODUCTION: The Cancer Genome Atlas (TCGA) project and Asian Cancer Research Group (ACRG) recently categorized gastric cancer into molecular subtypes. Nevertheless, these classification systems require high cost and sophisticated molecular technologies, preventing their widespread use in the clinic. This study is aimed to generating molecular subtypes of gastric cancer using techniques available in routine diagnostic practice in a series of Moroccan gastric cancer patients. In addition, we assessed the associations between molecular subtypes, clinicopathological features, and prognosis. METHODS: Ninety-seven gastric cancer cases were classified according to TCGA, ACRG, and integrated classifications using a panel of four molecular markers (EBV, MSI, E-cadherin, and p53). HER2 status and PD-L1 expression were also evaluated. These markers were analyzed using immunohistochemistry (E-cadherin, p53, HER2, and PD-L1), in situ hybridization (EBV and HER2 equivocal cases), and multiplex PCR (MSI). RESULTS: Our results showed that the subtypes presented distinct clinicopathological features and prognosis. EBV-positive gastric cancers were found exclusively in male patients. The GS (TCGA classification), MSS/EMT (ACRG classification), and E-cadherin aberrant subtype (integrated classification) presented the Lauren diffuse histology enrichment and tended to be diagnosed at a younger age. The MSI subtype was associated with a better overall survival across all classifications (TCGA, ACRG, and integrated classification). The worst prognosis was observed in the EBV subtype (TCGA and integrated classification) and MSS/EMT subtype (ACRG classification). Discussion/Conclusion. We reported a reproducible and affordable gastric cancer subtyping algorithms that can reproduce the recently recognized TCGA, ACRG, and integrated gastric cancer classifications, using techniques available in routine diagnosis. These simplified classifications can be employed not only for molecular classification but also in predicting the prognosis of gastric cancer patients. Hindawi 2021-07-28 /pmc/articles/PMC8342151/ /pubmed/34367379 http://dx.doi.org/10.1155/2021/9980410 Text en Copyright © 2021 Jean Paul Nshizirungu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nshizirungu, Jean Paul
Bennis, Sanae
Mellouki, Ihsane
Sekal, Mohammed
Benajah, Dafr-Allah
Lahmidani, Nada
El Bouhaddouti, Hicham
Ibn Majdoub, Karim
Ibrahimi, Sidi Adil
Celeiro, Sónia Pires
Viana-Pereira, Marta
Munari, Fernanda Franco
Ribeiro, Guilherme Gomes
Duval, Vinicius
Santana, Iara
Reis, Rui Manuel
Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients
title Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients
title_full Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients
title_fullStr Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients
title_full_unstemmed Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients
title_short Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients
title_sort reproduction of the cancer genome atlas (tcga) and asian cancer research group (acrg) gastric cancer molecular classifications and their association with clinicopathological characteristics and overall survival in moroccan patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342151/
https://www.ncbi.nlm.nih.gov/pubmed/34367379
http://dx.doi.org/10.1155/2021/9980410
work_keys_str_mv AT nshizirungujeanpaul reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT bennissanae reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT melloukiihsane reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT sekalmohammed reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT benajahdafrallah reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT lahmidaninada reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT elbouhaddoutihicham reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT ibnmajdoubkarim reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT ibrahimisidiadil reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT celeirosoniapires reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT vianapereiramarta reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT munarifernandafranco reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT ribeiroguilhermegomes reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT duvalvinicius reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT santanaiara reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients
AT reisruimanuel reproductionofthecancergenomeatlastcgaandasiancancerresearchgroupacrggastriccancermolecularclassificationsandtheirassociationwithclinicopathologicalcharacteristicsandoverallsurvivalinmoroccanpatients