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Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients
INTRODUCTION: The Cancer Genome Atlas (TCGA) project and Asian Cancer Research Group (ACRG) recently categorized gastric cancer into molecular subtypes. Nevertheless, these classification systems require high cost and sophisticated molecular technologies, preventing their widespread use in the clini...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342151/ https://www.ncbi.nlm.nih.gov/pubmed/34367379 http://dx.doi.org/10.1155/2021/9980410 |
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author | Nshizirungu, Jean Paul Bennis, Sanae Mellouki, Ihsane Sekal, Mohammed Benajah, Dafr-Allah Lahmidani, Nada El Bouhaddouti, Hicham Ibn Majdoub, Karim Ibrahimi, Sidi Adil Celeiro, Sónia Pires Viana-Pereira, Marta Munari, Fernanda Franco Ribeiro, Guilherme Gomes Duval, Vinicius Santana, Iara Reis, Rui Manuel |
author_facet | Nshizirungu, Jean Paul Bennis, Sanae Mellouki, Ihsane Sekal, Mohammed Benajah, Dafr-Allah Lahmidani, Nada El Bouhaddouti, Hicham Ibn Majdoub, Karim Ibrahimi, Sidi Adil Celeiro, Sónia Pires Viana-Pereira, Marta Munari, Fernanda Franco Ribeiro, Guilherme Gomes Duval, Vinicius Santana, Iara Reis, Rui Manuel |
author_sort | Nshizirungu, Jean Paul |
collection | PubMed |
description | INTRODUCTION: The Cancer Genome Atlas (TCGA) project and Asian Cancer Research Group (ACRG) recently categorized gastric cancer into molecular subtypes. Nevertheless, these classification systems require high cost and sophisticated molecular technologies, preventing their widespread use in the clinic. This study is aimed to generating molecular subtypes of gastric cancer using techniques available in routine diagnostic practice in a series of Moroccan gastric cancer patients. In addition, we assessed the associations between molecular subtypes, clinicopathological features, and prognosis. METHODS: Ninety-seven gastric cancer cases were classified according to TCGA, ACRG, and integrated classifications using a panel of four molecular markers (EBV, MSI, E-cadherin, and p53). HER2 status and PD-L1 expression were also evaluated. These markers were analyzed using immunohistochemistry (E-cadherin, p53, HER2, and PD-L1), in situ hybridization (EBV and HER2 equivocal cases), and multiplex PCR (MSI). RESULTS: Our results showed that the subtypes presented distinct clinicopathological features and prognosis. EBV-positive gastric cancers were found exclusively in male patients. The GS (TCGA classification), MSS/EMT (ACRG classification), and E-cadherin aberrant subtype (integrated classification) presented the Lauren diffuse histology enrichment and tended to be diagnosed at a younger age. The MSI subtype was associated with a better overall survival across all classifications (TCGA, ACRG, and integrated classification). The worst prognosis was observed in the EBV subtype (TCGA and integrated classification) and MSS/EMT subtype (ACRG classification). Discussion/Conclusion. We reported a reproducible and affordable gastric cancer subtyping algorithms that can reproduce the recently recognized TCGA, ACRG, and integrated gastric cancer classifications, using techniques available in routine diagnosis. These simplified classifications can be employed not only for molecular classification but also in predicting the prognosis of gastric cancer patients. |
format | Online Article Text |
id | pubmed-8342151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-83421512021-08-06 Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients Nshizirungu, Jean Paul Bennis, Sanae Mellouki, Ihsane Sekal, Mohammed Benajah, Dafr-Allah Lahmidani, Nada El Bouhaddouti, Hicham Ibn Majdoub, Karim Ibrahimi, Sidi Adil Celeiro, Sónia Pires Viana-Pereira, Marta Munari, Fernanda Franco Ribeiro, Guilherme Gomes Duval, Vinicius Santana, Iara Reis, Rui Manuel Dis Markers Research Article INTRODUCTION: The Cancer Genome Atlas (TCGA) project and Asian Cancer Research Group (ACRG) recently categorized gastric cancer into molecular subtypes. Nevertheless, these classification systems require high cost and sophisticated molecular technologies, preventing their widespread use in the clinic. This study is aimed to generating molecular subtypes of gastric cancer using techniques available in routine diagnostic practice in a series of Moroccan gastric cancer patients. In addition, we assessed the associations between molecular subtypes, clinicopathological features, and prognosis. METHODS: Ninety-seven gastric cancer cases were classified according to TCGA, ACRG, and integrated classifications using a panel of four molecular markers (EBV, MSI, E-cadherin, and p53). HER2 status and PD-L1 expression were also evaluated. These markers were analyzed using immunohistochemistry (E-cadherin, p53, HER2, and PD-L1), in situ hybridization (EBV and HER2 equivocal cases), and multiplex PCR (MSI). RESULTS: Our results showed that the subtypes presented distinct clinicopathological features and prognosis. EBV-positive gastric cancers were found exclusively in male patients. The GS (TCGA classification), MSS/EMT (ACRG classification), and E-cadherin aberrant subtype (integrated classification) presented the Lauren diffuse histology enrichment and tended to be diagnosed at a younger age. The MSI subtype was associated with a better overall survival across all classifications (TCGA, ACRG, and integrated classification). The worst prognosis was observed in the EBV subtype (TCGA and integrated classification) and MSS/EMT subtype (ACRG classification). Discussion/Conclusion. We reported a reproducible and affordable gastric cancer subtyping algorithms that can reproduce the recently recognized TCGA, ACRG, and integrated gastric cancer classifications, using techniques available in routine diagnosis. These simplified classifications can be employed not only for molecular classification but also in predicting the prognosis of gastric cancer patients. Hindawi 2021-07-28 /pmc/articles/PMC8342151/ /pubmed/34367379 http://dx.doi.org/10.1155/2021/9980410 Text en Copyright © 2021 Jean Paul Nshizirungu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nshizirungu, Jean Paul Bennis, Sanae Mellouki, Ihsane Sekal, Mohammed Benajah, Dafr-Allah Lahmidani, Nada El Bouhaddouti, Hicham Ibn Majdoub, Karim Ibrahimi, Sidi Adil Celeiro, Sónia Pires Viana-Pereira, Marta Munari, Fernanda Franco Ribeiro, Guilherme Gomes Duval, Vinicius Santana, Iara Reis, Rui Manuel Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients |
title | Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients |
title_full | Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients |
title_fullStr | Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients |
title_full_unstemmed | Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients |
title_short | Reproduction of the Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) Gastric Cancer Molecular Classifications and Their Association with Clinicopathological Characteristics and Overall Survival in Moroccan Patients |
title_sort | reproduction of the cancer genome atlas (tcga) and asian cancer research group (acrg) gastric cancer molecular classifications and their association with clinicopathological characteristics and overall survival in moroccan patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342151/ https://www.ncbi.nlm.nih.gov/pubmed/34367379 http://dx.doi.org/10.1155/2021/9980410 |
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