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Laminin-511-derived recombinant fragment and Rho kinase inhibitor Y-27632 facilitate serial cultivation of keratinocytes differentiated from human embryonic stem cells
INTRODUCTION: Keratinocytes derived from pluripotent stem cells have a short proliferative lifespan under conventional culture conditions that are optimized for keratinocytes. Recently, a Rho kinase inhibitor, Y-27632, had been used as a standard supplement for culture medium in which the proliferat...
Autores principales: | Takagi, Ryo, Tanuma-Takahashi, Akiko, Akiyama, Saeko, Kaneko, Wakana, Miura, Chika, Yamato, Masayuki, Shimizu, Tatsuya, Umezawa, Akihiro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society for Regenerative Medicine
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342860/ https://www.ncbi.nlm.nih.gov/pubmed/34409136 http://dx.doi.org/10.1016/j.reth.2021.07.004 |
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