Cargando…
Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia
Preeclampsia (PE) is a hypertensive disorder that affects 2–8% of pregnancies and is one of the main causes of fetal, neonatal, and maternal mortality and morbidity worldwide. Although PE etiology and pathophysiology remain unknown, there is evidence that the hyperactivation of maternal immunity cel...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342945/ https://www.ncbi.nlm.nih.gov/pubmed/34366896 http://dx.doi.org/10.3389/fphys.2021.708824 |
_version_ | 1783734171712094208 |
---|---|
author | Ayala-Ramírez, Paola Machuca-Acevedo, Catalina Gámez, Tatiana Quijano, Sandra Barreto, Alfonso Silva, Jaime L. Olaya-C, Mercedes García-Robles, Reggie |
author_facet | Ayala-Ramírez, Paola Machuca-Acevedo, Catalina Gámez, Tatiana Quijano, Sandra Barreto, Alfonso Silva, Jaime L. Olaya-C, Mercedes García-Robles, Reggie |
author_sort | Ayala-Ramírez, Paola |
collection | PubMed |
description | Preeclampsia (PE) is a hypertensive disorder that affects 2–8% of pregnancies and is one of the main causes of fetal, neonatal, and maternal mortality and morbidity worldwide. Although PE etiology and pathophysiology remain unknown, there is evidence that the hyperactivation of maternal immunity cells against placental cells triggers trophoblast cell apoptosis and death. It has also been reported that placenta-derived extracellular vesicles (EV) carry Fas ligand (FasL) and Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and trigger apoptosis in Jurkat T cells. This study aimed to quantify and compare FasL and TRAIL expression in EV derived from cultures of placenta explants from women with PE (early versus late) and women with uncomplicated pregnancies. Also, the study assessed EV capacity to induce apoptosis in Jurkat T cells. The authors isolated EV from placenta explant cultures, quantified FasL and TRAIL using ELISA, and analyzed EV apoptosis-inducing capability by flow cytometry. Results showed increased FasL and TRAIL in EV derived from placenta of women with PE, and increased EV apoptosis-inducing capability in Jurkat T cells. These results offer supporting evidence that EV FasL and TRAIL play a role in the pathophysiology of PE. |
format | Online Article Text |
id | pubmed-8342945 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83429452021-08-07 Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia Ayala-Ramírez, Paola Machuca-Acevedo, Catalina Gámez, Tatiana Quijano, Sandra Barreto, Alfonso Silva, Jaime L. Olaya-C, Mercedes García-Robles, Reggie Front Physiol Physiology Preeclampsia (PE) is a hypertensive disorder that affects 2–8% of pregnancies and is one of the main causes of fetal, neonatal, and maternal mortality and morbidity worldwide. Although PE etiology and pathophysiology remain unknown, there is evidence that the hyperactivation of maternal immunity cells against placental cells triggers trophoblast cell apoptosis and death. It has also been reported that placenta-derived extracellular vesicles (EV) carry Fas ligand (FasL) and Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and trigger apoptosis in Jurkat T cells. This study aimed to quantify and compare FasL and TRAIL expression in EV derived from cultures of placenta explants from women with PE (early versus late) and women with uncomplicated pregnancies. Also, the study assessed EV capacity to induce apoptosis in Jurkat T cells. The authors isolated EV from placenta explant cultures, quantified FasL and TRAIL using ELISA, and analyzed EV apoptosis-inducing capability by flow cytometry. Results showed increased FasL and TRAIL in EV derived from placenta of women with PE, and increased EV apoptosis-inducing capability in Jurkat T cells. These results offer supporting evidence that EV FasL and TRAIL play a role in the pathophysiology of PE. Frontiers Media S.A. 2021-07-23 /pmc/articles/PMC8342945/ /pubmed/34366896 http://dx.doi.org/10.3389/fphys.2021.708824 Text en Copyright © 2021 Ayala-Ramírez, Machuca-Acevedo, Gámez, Quijano, Barreto, Silva, Olaya-C and García-Robles. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Ayala-Ramírez, Paola Machuca-Acevedo, Catalina Gámez, Tatiana Quijano, Sandra Barreto, Alfonso Silva, Jaime L. Olaya-C, Mercedes García-Robles, Reggie Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia |
title | Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia |
title_full | Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia |
title_fullStr | Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia |
title_full_unstemmed | Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia |
title_short | Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia |
title_sort | assessment of placental extracellular vesicles-associated fas ligand and tnf-related apoptosis-inducing ligand in pregnancies complicated by early and late onset preeclampsia |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342945/ https://www.ncbi.nlm.nih.gov/pubmed/34366896 http://dx.doi.org/10.3389/fphys.2021.708824 |
work_keys_str_mv | AT ayalaramirezpaola assessmentofplacentalextracellularvesiclesassociatedfasligandandtnfrelatedapoptosisinducingligandinpregnanciescomplicatedbyearlyandlateonsetpreeclampsia AT machucaacevedocatalina assessmentofplacentalextracellularvesiclesassociatedfasligandandtnfrelatedapoptosisinducingligandinpregnanciescomplicatedbyearlyandlateonsetpreeclampsia AT gameztatiana assessmentofplacentalextracellularvesiclesassociatedfasligandandtnfrelatedapoptosisinducingligandinpregnanciescomplicatedbyearlyandlateonsetpreeclampsia AT quijanosandra assessmentofplacentalextracellularvesiclesassociatedfasligandandtnfrelatedapoptosisinducingligandinpregnanciescomplicatedbyearlyandlateonsetpreeclampsia AT barretoalfonso assessmentofplacentalextracellularvesiclesassociatedfasligandandtnfrelatedapoptosisinducingligandinpregnanciescomplicatedbyearlyandlateonsetpreeclampsia AT silvajaimel assessmentofplacentalextracellularvesiclesassociatedfasligandandtnfrelatedapoptosisinducingligandinpregnanciescomplicatedbyearlyandlateonsetpreeclampsia AT olayacmercedes assessmentofplacentalextracellularvesiclesassociatedfasligandandtnfrelatedapoptosisinducingligandinpregnanciescomplicatedbyearlyandlateonsetpreeclampsia AT garciaroblesreggie assessmentofplacentalextracellularvesiclesassociatedfasligandandtnfrelatedapoptosisinducingligandinpregnanciescomplicatedbyearlyandlateonsetpreeclampsia |