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Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia

Foot-and-mouth disease virus (FMDV) is widespread throughout much of the world, including parts of South East Asia. Surveillance is often limited in endemic areas, relying predominantly on passive outbreak reporting. As part of the World Organisation for Animal Health (OIE)'s South East Asia an...

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Autores principales: Buckle, Kelly, Bueno, Rudolfo, McFadden, Andrew, van Andel, Mary, Spence, Richard, Hamill, Carolyn, Roe, Wendi, Vallee, Emilie, Castillo-Alcala, Fernanda, Abila, Ronel, Verin, Blesilda, Purevsuren, Bolortuya, Sutar, Ashish, Win, Htun Htun, Thiha, Myo, Lwin, Khin Ohnmar, Khounsy, Syseng, Phonthasy, Sengxay, Souriya, Viliddeth, Keokhamphet, Chattouphone, Arzt, Jonathan, Ludi, Anna, Mioulet, Valérie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342991/
https://www.ncbi.nlm.nih.gov/pubmed/34368278
http://dx.doi.org/10.3389/fvets.2021.691308
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author Buckle, Kelly
Bueno, Rudolfo
McFadden, Andrew
van Andel, Mary
Spence, Richard
Hamill, Carolyn
Roe, Wendi
Vallee, Emilie
Castillo-Alcala, Fernanda
Abila, Ronel
Verin, Blesilda
Purevsuren, Bolortuya
Sutar, Ashish
Win, Htun Htun
Thiha, Myo
Lwin, Khin Ohnmar
Khounsy, Syseng
Phonthasy, Sengxay
Souriya, Viliddeth
Keokhamphet, Chattouphone
Arzt, Jonathan
Ludi, Anna
Mioulet, Valérie
author_facet Buckle, Kelly
Bueno, Rudolfo
McFadden, Andrew
van Andel, Mary
Spence, Richard
Hamill, Carolyn
Roe, Wendi
Vallee, Emilie
Castillo-Alcala, Fernanda
Abila, Ronel
Verin, Blesilda
Purevsuren, Bolortuya
Sutar, Ashish
Win, Htun Htun
Thiha, Myo
Lwin, Khin Ohnmar
Khounsy, Syseng
Phonthasy, Sengxay
Souriya, Viliddeth
Keokhamphet, Chattouphone
Arzt, Jonathan
Ludi, Anna
Mioulet, Valérie
author_sort Buckle, Kelly
collection PubMed
description Foot-and-mouth disease virus (FMDV) is widespread throughout much of the world, including parts of South East Asia. Surveillance is often limited in endemic areas, relying predominantly on passive outbreak reporting. As part of the World Organisation for Animal Health (OIE)'s South East Asia and China Foot-and-Mouth Disease Project (SEACFMD), field sampling was performed to help understand evidence of widespread virus exposure observed in previous studies. Serum and dry mucosal swabs were collected to evaluate the presence of FMDV RNA on the nasal, oral, and dorsal nasopharyngeal mucosal surfaces of 262 healthy cattle (n = 84 in Laos; n = 125 in Myanmar) and buffalo (n = 48 in Laos; n = 5 in Myanmar) immediately following slaughter in three slaughterhouses. Swabs and serum were tested by the OIE/FAO World Reference Laboratory for foot-and-mouth disease (WRLFMD) using pan-serotypic real-time reverse transcription-PCR (rRT-PCR) and serum was evaluated using the FMD PrioCHECK non-structural protein (NSP) ELISA. In total, 7.3% of animals had detectable FMDV RNA in one or more of the three sites including 5.3% of nasopharyngeal swabs, 2.3% of oral swabs, and 1.5% of nasal swabs. No FMDV RNA was detected in serum. Overall, 37.8% of animals were positive for NSP antibodies, indicating likely past natural exposure to FMDV. Results were comparable for Laos and Myanmar, and for both cattle and buffalo, and were not significantly different between age groups. Detectable FMDV RNA present on the oral and nasal mucosa of clinically-healthy large ruminants in Laos and Myanmar demonstrates the importance of sampling asymptomatic animals as part of surveillance, and may indicate that subclinical infection plays a role in the epidemiology of FMD in these countries.
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spelling pubmed-83429912021-08-07 Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia Buckle, Kelly Bueno, Rudolfo McFadden, Andrew van Andel, Mary Spence, Richard Hamill, Carolyn Roe, Wendi Vallee, Emilie Castillo-Alcala, Fernanda Abila, Ronel Verin, Blesilda Purevsuren, Bolortuya Sutar, Ashish Win, Htun Htun Thiha, Myo Lwin, Khin Ohnmar Khounsy, Syseng Phonthasy, Sengxay Souriya, Viliddeth Keokhamphet, Chattouphone Arzt, Jonathan Ludi, Anna Mioulet, Valérie Front Vet Sci Veterinary Science Foot-and-mouth disease virus (FMDV) is widespread throughout much of the world, including parts of South East Asia. Surveillance is often limited in endemic areas, relying predominantly on passive outbreak reporting. As part of the World Organisation for Animal Health (OIE)'s South East Asia and China Foot-and-Mouth Disease Project (SEACFMD), field sampling was performed to help understand evidence of widespread virus exposure observed in previous studies. Serum and dry mucosal swabs were collected to evaluate the presence of FMDV RNA on the nasal, oral, and dorsal nasopharyngeal mucosal surfaces of 262 healthy cattle (n = 84 in Laos; n = 125 in Myanmar) and buffalo (n = 48 in Laos; n = 5 in Myanmar) immediately following slaughter in three slaughterhouses. Swabs and serum were tested by the OIE/FAO World Reference Laboratory for foot-and-mouth disease (WRLFMD) using pan-serotypic real-time reverse transcription-PCR (rRT-PCR) and serum was evaluated using the FMD PrioCHECK non-structural protein (NSP) ELISA. In total, 7.3% of animals had detectable FMDV RNA in one or more of the three sites including 5.3% of nasopharyngeal swabs, 2.3% of oral swabs, and 1.5% of nasal swabs. No FMDV RNA was detected in serum. Overall, 37.8% of animals were positive for NSP antibodies, indicating likely past natural exposure to FMDV. Results were comparable for Laos and Myanmar, and for both cattle and buffalo, and were not significantly different between age groups. Detectable FMDV RNA present on the oral and nasal mucosa of clinically-healthy large ruminants in Laos and Myanmar demonstrates the importance of sampling asymptomatic animals as part of surveillance, and may indicate that subclinical infection plays a role in the epidemiology of FMD in these countries. Frontiers Media S.A. 2021-07-23 /pmc/articles/PMC8342991/ /pubmed/34368278 http://dx.doi.org/10.3389/fvets.2021.691308 Text en Copyright © 2021 Buckle, Bueno, McFadden, van Andel, Spence, Hamill, Roe, Vallee, Castillo-Alcala, Abila, Verin, Purevsuren, Sutar, Win, Thiha, Lwin, Khounsy, Phonthasy, Souriya, Keokhamphet, Arzt, Ludi and Mioulet. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Buckle, Kelly
Bueno, Rudolfo
McFadden, Andrew
van Andel, Mary
Spence, Richard
Hamill, Carolyn
Roe, Wendi
Vallee, Emilie
Castillo-Alcala, Fernanda
Abila, Ronel
Verin, Blesilda
Purevsuren, Bolortuya
Sutar, Ashish
Win, Htun Htun
Thiha, Myo
Lwin, Khin Ohnmar
Khounsy, Syseng
Phonthasy, Sengxay
Souriya, Viliddeth
Keokhamphet, Chattouphone
Arzt, Jonathan
Ludi, Anna
Mioulet, Valérie
Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia
title Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia
title_full Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia
title_fullStr Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia
title_full_unstemmed Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia
title_short Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia
title_sort detection of foot-and-mouth disease virus in the absence of clinical disease in cattle and buffalo in south east asia
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342991/
https://www.ncbi.nlm.nih.gov/pubmed/34368278
http://dx.doi.org/10.3389/fvets.2021.691308
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