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Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia
Foot-and-mouth disease virus (FMDV) is widespread throughout much of the world, including parts of South East Asia. Surveillance is often limited in endemic areas, relying predominantly on passive outbreak reporting. As part of the World Organisation for Animal Health (OIE)'s South East Asia an...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342991/ https://www.ncbi.nlm.nih.gov/pubmed/34368278 http://dx.doi.org/10.3389/fvets.2021.691308 |
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author | Buckle, Kelly Bueno, Rudolfo McFadden, Andrew van Andel, Mary Spence, Richard Hamill, Carolyn Roe, Wendi Vallee, Emilie Castillo-Alcala, Fernanda Abila, Ronel Verin, Blesilda Purevsuren, Bolortuya Sutar, Ashish Win, Htun Htun Thiha, Myo Lwin, Khin Ohnmar Khounsy, Syseng Phonthasy, Sengxay Souriya, Viliddeth Keokhamphet, Chattouphone Arzt, Jonathan Ludi, Anna Mioulet, Valérie |
author_facet | Buckle, Kelly Bueno, Rudolfo McFadden, Andrew van Andel, Mary Spence, Richard Hamill, Carolyn Roe, Wendi Vallee, Emilie Castillo-Alcala, Fernanda Abila, Ronel Verin, Blesilda Purevsuren, Bolortuya Sutar, Ashish Win, Htun Htun Thiha, Myo Lwin, Khin Ohnmar Khounsy, Syseng Phonthasy, Sengxay Souriya, Viliddeth Keokhamphet, Chattouphone Arzt, Jonathan Ludi, Anna Mioulet, Valérie |
author_sort | Buckle, Kelly |
collection | PubMed |
description | Foot-and-mouth disease virus (FMDV) is widespread throughout much of the world, including parts of South East Asia. Surveillance is often limited in endemic areas, relying predominantly on passive outbreak reporting. As part of the World Organisation for Animal Health (OIE)'s South East Asia and China Foot-and-Mouth Disease Project (SEACFMD), field sampling was performed to help understand evidence of widespread virus exposure observed in previous studies. Serum and dry mucosal swabs were collected to evaluate the presence of FMDV RNA on the nasal, oral, and dorsal nasopharyngeal mucosal surfaces of 262 healthy cattle (n = 84 in Laos; n = 125 in Myanmar) and buffalo (n = 48 in Laos; n = 5 in Myanmar) immediately following slaughter in three slaughterhouses. Swabs and serum were tested by the OIE/FAO World Reference Laboratory for foot-and-mouth disease (WRLFMD) using pan-serotypic real-time reverse transcription-PCR (rRT-PCR) and serum was evaluated using the FMD PrioCHECK non-structural protein (NSP) ELISA. In total, 7.3% of animals had detectable FMDV RNA in one or more of the three sites including 5.3% of nasopharyngeal swabs, 2.3% of oral swabs, and 1.5% of nasal swabs. No FMDV RNA was detected in serum. Overall, 37.8% of animals were positive for NSP antibodies, indicating likely past natural exposure to FMDV. Results were comparable for Laos and Myanmar, and for both cattle and buffalo, and were not significantly different between age groups. Detectable FMDV RNA present on the oral and nasal mucosa of clinically-healthy large ruminants in Laos and Myanmar demonstrates the importance of sampling asymptomatic animals as part of surveillance, and may indicate that subclinical infection plays a role in the epidemiology of FMD in these countries. |
format | Online Article Text |
id | pubmed-8342991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83429912021-08-07 Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia Buckle, Kelly Bueno, Rudolfo McFadden, Andrew van Andel, Mary Spence, Richard Hamill, Carolyn Roe, Wendi Vallee, Emilie Castillo-Alcala, Fernanda Abila, Ronel Verin, Blesilda Purevsuren, Bolortuya Sutar, Ashish Win, Htun Htun Thiha, Myo Lwin, Khin Ohnmar Khounsy, Syseng Phonthasy, Sengxay Souriya, Viliddeth Keokhamphet, Chattouphone Arzt, Jonathan Ludi, Anna Mioulet, Valérie Front Vet Sci Veterinary Science Foot-and-mouth disease virus (FMDV) is widespread throughout much of the world, including parts of South East Asia. Surveillance is often limited in endemic areas, relying predominantly on passive outbreak reporting. As part of the World Organisation for Animal Health (OIE)'s South East Asia and China Foot-and-Mouth Disease Project (SEACFMD), field sampling was performed to help understand evidence of widespread virus exposure observed in previous studies. Serum and dry mucosal swabs were collected to evaluate the presence of FMDV RNA on the nasal, oral, and dorsal nasopharyngeal mucosal surfaces of 262 healthy cattle (n = 84 in Laos; n = 125 in Myanmar) and buffalo (n = 48 in Laos; n = 5 in Myanmar) immediately following slaughter in three slaughterhouses. Swabs and serum were tested by the OIE/FAO World Reference Laboratory for foot-and-mouth disease (WRLFMD) using pan-serotypic real-time reverse transcription-PCR (rRT-PCR) and serum was evaluated using the FMD PrioCHECK non-structural protein (NSP) ELISA. In total, 7.3% of animals had detectable FMDV RNA in one or more of the three sites including 5.3% of nasopharyngeal swabs, 2.3% of oral swabs, and 1.5% of nasal swabs. No FMDV RNA was detected in serum. Overall, 37.8% of animals were positive for NSP antibodies, indicating likely past natural exposure to FMDV. Results were comparable for Laos and Myanmar, and for both cattle and buffalo, and were not significantly different between age groups. Detectable FMDV RNA present on the oral and nasal mucosa of clinically-healthy large ruminants in Laos and Myanmar demonstrates the importance of sampling asymptomatic animals as part of surveillance, and may indicate that subclinical infection plays a role in the epidemiology of FMD in these countries. Frontiers Media S.A. 2021-07-23 /pmc/articles/PMC8342991/ /pubmed/34368278 http://dx.doi.org/10.3389/fvets.2021.691308 Text en Copyright © 2021 Buckle, Bueno, McFadden, van Andel, Spence, Hamill, Roe, Vallee, Castillo-Alcala, Abila, Verin, Purevsuren, Sutar, Win, Thiha, Lwin, Khounsy, Phonthasy, Souriya, Keokhamphet, Arzt, Ludi and Mioulet. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Buckle, Kelly Bueno, Rudolfo McFadden, Andrew van Andel, Mary Spence, Richard Hamill, Carolyn Roe, Wendi Vallee, Emilie Castillo-Alcala, Fernanda Abila, Ronel Verin, Blesilda Purevsuren, Bolortuya Sutar, Ashish Win, Htun Htun Thiha, Myo Lwin, Khin Ohnmar Khounsy, Syseng Phonthasy, Sengxay Souriya, Viliddeth Keokhamphet, Chattouphone Arzt, Jonathan Ludi, Anna Mioulet, Valérie Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia |
title | Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia |
title_full | Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia |
title_fullStr | Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia |
title_full_unstemmed | Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia |
title_short | Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia |
title_sort | detection of foot-and-mouth disease virus in the absence of clinical disease in cattle and buffalo in south east asia |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342991/ https://www.ncbi.nlm.nih.gov/pubmed/34368278 http://dx.doi.org/10.3389/fvets.2021.691308 |
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