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Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis
The worldwide pandemic of COVID-19 has become a global public health crisis. Various clinical diagnosis methods have been developed to distinguish COVID-19–infected patients from healthy people. The nucleic acid test is the golden standard for virus detection as it is suitable for early diagnosis. H...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343015/ https://www.ncbi.nlm.nih.gov/pubmed/34368226 http://dx.doi.org/10.3389/fmolb.2021.682405 |
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author | Gong, Fanwu Wei, Hua-xing Li, Qiangsheng Liu, Liu Li, Bofeng |
author_facet | Gong, Fanwu Wei, Hua-xing Li, Qiangsheng Liu, Liu Li, Bofeng |
author_sort | Gong, Fanwu |
collection | PubMed |
description | The worldwide pandemic of COVID-19 has become a global public health crisis. Various clinical diagnosis methods have been developed to distinguish COVID-19–infected patients from healthy people. The nucleic acid test is the golden standard for virus detection as it is suitable for early diagnosis. However, due to the low amount of viral nucleic acid in the respiratory tract, the sensitivity of nucleic acid detection is unsatisfactory. As a result, serological screening began to be widely used with the merits of simple procedures, lower cost, and shorter detection time. Serological tests currently include the enzyme-linked immunosorbent assay (ELISA), lateral flow immunoassay (LFIA), and chemiluminescence immunoassay (CLIA). This review describes various serological methods, discusses the performance and diagnostic effects of different methods, and points out the problems and the direction of optimization, to improve the efficiency of clinical diagnosis. These increasingly sophisticated and diverse serological diagnostic technologies will help human beings to control the spread of COVID-19. |
format | Online Article Text |
id | pubmed-8343015 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83430152021-08-07 Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis Gong, Fanwu Wei, Hua-xing Li, Qiangsheng Liu, Liu Li, Bofeng Front Mol Biosci Molecular Biosciences The worldwide pandemic of COVID-19 has become a global public health crisis. Various clinical diagnosis methods have been developed to distinguish COVID-19–infected patients from healthy people. The nucleic acid test is the golden standard for virus detection as it is suitable for early diagnosis. However, due to the low amount of viral nucleic acid in the respiratory tract, the sensitivity of nucleic acid detection is unsatisfactory. As a result, serological screening began to be widely used with the merits of simple procedures, lower cost, and shorter detection time. Serological tests currently include the enzyme-linked immunosorbent assay (ELISA), lateral flow immunoassay (LFIA), and chemiluminescence immunoassay (CLIA). This review describes various serological methods, discusses the performance and diagnostic effects of different methods, and points out the problems and the direction of optimization, to improve the efficiency of clinical diagnosis. These increasingly sophisticated and diverse serological diagnostic technologies will help human beings to control the spread of COVID-19. Frontiers Media S.A. 2021-07-23 /pmc/articles/PMC8343015/ /pubmed/34368226 http://dx.doi.org/10.3389/fmolb.2021.682405 Text en Copyright © 2021 Gong, Wei, Li, Liu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Gong, Fanwu Wei, Hua-xing Li, Qiangsheng Liu, Liu Li, Bofeng Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis |
title | Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis |
title_full | Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis |
title_fullStr | Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis |
title_full_unstemmed | Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis |
title_short | Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis |
title_sort | evaluation and comparison of serological methods for covid-19 diagnosis |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343015/ https://www.ncbi.nlm.nih.gov/pubmed/34368226 http://dx.doi.org/10.3389/fmolb.2021.682405 |
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