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Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis

The worldwide pandemic of COVID-19 has become a global public health crisis. Various clinical diagnosis methods have been developed to distinguish COVID-19–infected patients from healthy people. The nucleic acid test is the golden standard for virus detection as it is suitable for early diagnosis. H...

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Autores principales: Gong, Fanwu, Wei, Hua-xing, Li, Qiangsheng, Liu, Liu, Li, Bofeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343015/
https://www.ncbi.nlm.nih.gov/pubmed/34368226
http://dx.doi.org/10.3389/fmolb.2021.682405
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author Gong, Fanwu
Wei, Hua-xing
Li, Qiangsheng
Liu, Liu
Li, Bofeng
author_facet Gong, Fanwu
Wei, Hua-xing
Li, Qiangsheng
Liu, Liu
Li, Bofeng
author_sort Gong, Fanwu
collection PubMed
description The worldwide pandemic of COVID-19 has become a global public health crisis. Various clinical diagnosis methods have been developed to distinguish COVID-19–infected patients from healthy people. The nucleic acid test is the golden standard for virus detection as it is suitable for early diagnosis. However, due to the low amount of viral nucleic acid in the respiratory tract, the sensitivity of nucleic acid detection is unsatisfactory. As a result, serological screening began to be widely used with the merits of simple procedures, lower cost, and shorter detection time. Serological tests currently include the enzyme-linked immunosorbent assay (ELISA), lateral flow immunoassay (LFIA), and chemiluminescence immunoassay (CLIA). This review describes various serological methods, discusses the performance and diagnostic effects of different methods, and points out the problems and the direction of optimization, to improve the efficiency of clinical diagnosis. These increasingly sophisticated and diverse serological diagnostic technologies will help human beings to control the spread of COVID-19.
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spelling pubmed-83430152021-08-07 Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis Gong, Fanwu Wei, Hua-xing Li, Qiangsheng Liu, Liu Li, Bofeng Front Mol Biosci Molecular Biosciences The worldwide pandemic of COVID-19 has become a global public health crisis. Various clinical diagnosis methods have been developed to distinguish COVID-19–infected patients from healthy people. The nucleic acid test is the golden standard for virus detection as it is suitable for early diagnosis. However, due to the low amount of viral nucleic acid in the respiratory tract, the sensitivity of nucleic acid detection is unsatisfactory. As a result, serological screening began to be widely used with the merits of simple procedures, lower cost, and shorter detection time. Serological tests currently include the enzyme-linked immunosorbent assay (ELISA), lateral flow immunoassay (LFIA), and chemiluminescence immunoassay (CLIA). This review describes various serological methods, discusses the performance and diagnostic effects of different methods, and points out the problems and the direction of optimization, to improve the efficiency of clinical diagnosis. These increasingly sophisticated and diverse serological diagnostic technologies will help human beings to control the spread of COVID-19. Frontiers Media S.A. 2021-07-23 /pmc/articles/PMC8343015/ /pubmed/34368226 http://dx.doi.org/10.3389/fmolb.2021.682405 Text en Copyright © 2021 Gong, Wei, Li, Liu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Gong, Fanwu
Wei, Hua-xing
Li, Qiangsheng
Liu, Liu
Li, Bofeng
Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis
title Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis
title_full Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis
title_fullStr Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis
title_full_unstemmed Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis
title_short Evaluation and Comparison of Serological Methods for COVID-19 Diagnosis
title_sort evaluation and comparison of serological methods for covid-19 diagnosis
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343015/
https://www.ncbi.nlm.nih.gov/pubmed/34368226
http://dx.doi.org/10.3389/fmolb.2021.682405
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