Cargando…
NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma
NAP1L1 has been reported to be significantly involved in the carcinogenesis of hepatocellular carcinoma (HCC). Yet, its detailed molecular basis is still to be determined. Based on the analysis of The Cancer Genome Atlas (TCGA) database, NAP1L1 mRNA was found to be upregulated and predicted the poor...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343235/ https://www.ncbi.nlm.nih.gov/pubmed/34368121 http://dx.doi.org/10.3389/fcell.2021.659680 |
_version_ | 1783734242293841920 |
---|---|
author | Zhang, Ye-wei Chen, Qian Li, Bo Li, Hai-Yang Zhao, Xue-Ke Xiao, Yan-yi Liu, Shu Zuo, Shi |
author_facet | Zhang, Ye-wei Chen, Qian Li, Bo Li, Hai-Yang Zhao, Xue-Ke Xiao, Yan-yi Liu, Shu Zuo, Shi |
author_sort | Zhang, Ye-wei |
collection | PubMed |
description | NAP1L1 has been reported to be significantly involved in the carcinogenesis of hepatocellular carcinoma (HCC). Yet, its detailed molecular basis is still to be determined. Based on the analysis of The Cancer Genome Atlas (TCGA) database, NAP1L1 mRNA was found to be upregulated and predicted the poor prognosis initially. Subsequently, consistent with the prediction, the upregulated expression of NAP1L1 mRNA and protein levels was confirmed by quantitative polymerase chain reaction (qPCR), Western blot, and immunohistochemistry assays. Upregulated NAP1L1 protein positively promoted the disease progression and poor prognosis of HCC. In addition, NAP1L1 protein expression was considered as an independent prognostic factor in HCC. Inhibition of NAP1L1 expression by siRNA or shRNA pathway significantly reduced the cell proliferation and cell cycle transformation in vitro and in vivo. Mechanism analysis first showed that the function of NAP1L1 was to recruit hepatoma-derived growth factor (HDGF), an oncogene candidate widely documented in tumors. Furthermore, the latter interacted with c-Jun, a key oncogenic transcription factor that can induce the expression of cell cycle factors and thus stimulate the cell growth in HCC. Finally, transfecting HDGF or c-Jun could reverse the suppressive effects on HCC growth in NAP1L1-suppressed HCC cells. Our data indicate that NAP1L1 is a potential oncogene and acts via recruiting HDGF/c-Jun in HCC. |
format | Online Article Text |
id | pubmed-8343235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83432352021-08-07 NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma Zhang, Ye-wei Chen, Qian Li, Bo Li, Hai-Yang Zhao, Xue-Ke Xiao, Yan-yi Liu, Shu Zuo, Shi Front Cell Dev Biol Cell and Developmental Biology NAP1L1 has been reported to be significantly involved in the carcinogenesis of hepatocellular carcinoma (HCC). Yet, its detailed molecular basis is still to be determined. Based on the analysis of The Cancer Genome Atlas (TCGA) database, NAP1L1 mRNA was found to be upregulated and predicted the poor prognosis initially. Subsequently, consistent with the prediction, the upregulated expression of NAP1L1 mRNA and protein levels was confirmed by quantitative polymerase chain reaction (qPCR), Western blot, and immunohistochemistry assays. Upregulated NAP1L1 protein positively promoted the disease progression and poor prognosis of HCC. In addition, NAP1L1 protein expression was considered as an independent prognostic factor in HCC. Inhibition of NAP1L1 expression by siRNA or shRNA pathway significantly reduced the cell proliferation and cell cycle transformation in vitro and in vivo. Mechanism analysis first showed that the function of NAP1L1 was to recruit hepatoma-derived growth factor (HDGF), an oncogene candidate widely documented in tumors. Furthermore, the latter interacted with c-Jun, a key oncogenic transcription factor that can induce the expression of cell cycle factors and thus stimulate the cell growth in HCC. Finally, transfecting HDGF or c-Jun could reverse the suppressive effects on HCC growth in NAP1L1-suppressed HCC cells. Our data indicate that NAP1L1 is a potential oncogene and acts via recruiting HDGF/c-Jun in HCC. Frontiers Media S.A. 2021-07-23 /pmc/articles/PMC8343235/ /pubmed/34368121 http://dx.doi.org/10.3389/fcell.2021.659680 Text en Copyright © 2021 Zhang, Chen, Li, Li, Zhao, Xiao, Liu and Zuo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Zhang, Ye-wei Chen, Qian Li, Bo Li, Hai-Yang Zhao, Xue-Ke Xiao, Yan-yi Liu, Shu Zuo, Shi NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma |
title | NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma |
title_full | NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma |
title_fullStr | NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma |
title_full_unstemmed | NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma |
title_short | NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma |
title_sort | nap1l1 functions as a tumor promoter via recruiting hepatoma-derived growth factor/c-jun signal in hepatocellular carcinoma |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343235/ https://www.ncbi.nlm.nih.gov/pubmed/34368121 http://dx.doi.org/10.3389/fcell.2021.659680 |
work_keys_str_mv | AT zhangyewei nap1l1functionsasatumorpromoterviarecruitinghepatomaderivedgrowthfactorcjunsignalinhepatocellularcarcinoma AT chenqian nap1l1functionsasatumorpromoterviarecruitinghepatomaderivedgrowthfactorcjunsignalinhepatocellularcarcinoma AT libo nap1l1functionsasatumorpromoterviarecruitinghepatomaderivedgrowthfactorcjunsignalinhepatocellularcarcinoma AT lihaiyang nap1l1functionsasatumorpromoterviarecruitinghepatomaderivedgrowthfactorcjunsignalinhepatocellularcarcinoma AT zhaoxueke nap1l1functionsasatumorpromoterviarecruitinghepatomaderivedgrowthfactorcjunsignalinhepatocellularcarcinoma AT xiaoyanyi nap1l1functionsasatumorpromoterviarecruitinghepatomaderivedgrowthfactorcjunsignalinhepatocellularcarcinoma AT liushu nap1l1functionsasatumorpromoterviarecruitinghepatomaderivedgrowthfactorcjunsignalinhepatocellularcarcinoma AT zuoshi nap1l1functionsasatumorpromoterviarecruitinghepatomaderivedgrowthfactorcjunsignalinhepatocellularcarcinoma |