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Treadmill exercise influences the microRNA profiles in the bone tissues of mice

As an important regulator involved in cell activity, microRNAs (miRNAs) are important in the process of exercise influencing bone metabolism. The present study aimed to detect and select differentially expressed miRNAs in the bone tissues of mice trained on a treadmill, predict the target genes of t...

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Autores principales: Yang, Huan, Cao, Zhen, Wang, Yang, Wang, Jiahui, Gao, Jintao, Han, Biao, Yu, Fangmei, Qin, Yixiong, Guo, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343800/
https://www.ncbi.nlm.nih.gov/pubmed/34373721
http://dx.doi.org/10.3892/etm.2021.10467
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author Yang, Huan
Cao, Zhen
Wang, Yang
Wang, Jiahui
Gao, Jintao
Han, Biao
Yu, Fangmei
Qin, Yixiong
Guo, Yong
author_facet Yang, Huan
Cao, Zhen
Wang, Yang
Wang, Jiahui
Gao, Jintao
Han, Biao
Yu, Fangmei
Qin, Yixiong
Guo, Yong
author_sort Yang, Huan
collection PubMed
description As an important regulator involved in cell activity, microRNAs (miRNAs) are important in the process of exercise influencing bone metabolism. The present study aimed to detect and select differentially expressed miRNAs in the bone tissues of mice trained on a treadmill, predict the target genes of these differentially expressed miRNAs and lay a foundation for exploring the effect of treadmill training on bone metabolism through miRNAs. In this experiment, after the mice were trained on a treadmill for 8 weeks, the mechanical properties of mouse femur bone were assessed, and the alkaline phosphatase (ALP) activity and osteocalcin (OCN) protein levels of the bone were assayed. miRNA microarray and reverse transcription-quantitative (RT-q)PCR were performed to select and validate differentially expressed miRNAs in the bone, and the target genes of these miRNAs were predicted with bioinformatics methods. In addition, the differentially expressed miRNAs in the bone tissues were compared with those in mechanically strained osteocytes in vitro. Treadmill training improved the mechanical properties of the femur bones of mice, and elevated the ALP activity and OCN protein level in the bone. In addition, 122 differentially expressed miRNAs were detected in the bone, of which nine were validated via RT-qPCR. Among the target genes of these differentially expressed miRNAs, certain candidates were involved in bone metabolism. A total of eight miRNAs were differentially expressed in both bone tissue and osteocytes, exhibiting the same expression trends, and various target genes of these eight miRNAs were also involved in bone metabolism. Treadmill training resulted in altered miRNA expression profiles in the bones of mice (mainly in osteocytes) and the differentially expressed miRNAs may serve important roles in regulating bone metabolism and osteogenic differentiation.
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spelling pubmed-83438002021-08-08 Treadmill exercise influences the microRNA profiles in the bone tissues of mice Yang, Huan Cao, Zhen Wang, Yang Wang, Jiahui Gao, Jintao Han, Biao Yu, Fangmei Qin, Yixiong Guo, Yong Exp Ther Med Articles As an important regulator involved in cell activity, microRNAs (miRNAs) are important in the process of exercise influencing bone metabolism. The present study aimed to detect and select differentially expressed miRNAs in the bone tissues of mice trained on a treadmill, predict the target genes of these differentially expressed miRNAs and lay a foundation for exploring the effect of treadmill training on bone metabolism through miRNAs. In this experiment, after the mice were trained on a treadmill for 8 weeks, the mechanical properties of mouse femur bone were assessed, and the alkaline phosphatase (ALP) activity and osteocalcin (OCN) protein levels of the bone were assayed. miRNA microarray and reverse transcription-quantitative (RT-q)PCR were performed to select and validate differentially expressed miRNAs in the bone, and the target genes of these miRNAs were predicted with bioinformatics methods. In addition, the differentially expressed miRNAs in the bone tissues were compared with those in mechanically strained osteocytes in vitro. Treadmill training improved the mechanical properties of the femur bones of mice, and elevated the ALP activity and OCN protein level in the bone. In addition, 122 differentially expressed miRNAs were detected in the bone, of which nine were validated via RT-qPCR. Among the target genes of these differentially expressed miRNAs, certain candidates were involved in bone metabolism. A total of eight miRNAs were differentially expressed in both bone tissue and osteocytes, exhibiting the same expression trends, and various target genes of these eight miRNAs were also involved in bone metabolism. Treadmill training resulted in altered miRNA expression profiles in the bones of mice (mainly in osteocytes) and the differentially expressed miRNAs may serve important roles in regulating bone metabolism and osteogenic differentiation. D.A. Spandidos 2021-09 2021-07-19 /pmc/articles/PMC8343800/ /pubmed/34373721 http://dx.doi.org/10.3892/etm.2021.10467 Text en Copyright: © Yang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yang, Huan
Cao, Zhen
Wang, Yang
Wang, Jiahui
Gao, Jintao
Han, Biao
Yu, Fangmei
Qin, Yixiong
Guo, Yong
Treadmill exercise influences the microRNA profiles in the bone tissues of mice
title Treadmill exercise influences the microRNA profiles in the bone tissues of mice
title_full Treadmill exercise influences the microRNA profiles in the bone tissues of mice
title_fullStr Treadmill exercise influences the microRNA profiles in the bone tissues of mice
title_full_unstemmed Treadmill exercise influences the microRNA profiles in the bone tissues of mice
title_short Treadmill exercise influences the microRNA profiles in the bone tissues of mice
title_sort treadmill exercise influences the microrna profiles in the bone tissues of mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343800/
https://www.ncbi.nlm.nih.gov/pubmed/34373721
http://dx.doi.org/10.3892/etm.2021.10467
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