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The Screening of Critical Related Genes in Celiac Disease Based on Intraepithelial Lymphocytes Investigation: A Bioinformatics Analysis

BACKGROUND: Celiac disease (CD) is an immunological intestinal disorder, which is characterized by response to gluten. In addition to the environmental factors and dysbiosis of the gut microbiota, genetic susceptibility has an important role in the pathogenesis of this multifactorial disorder. There...

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Autores principales: Rostami-Nejad, Mohammad, vafaee, Reza, Ehsani-Ardakani, Mohammad Javad, Aghamohammadi, Nika, Keramatinia, Aliasghar, Abdi, Saeed, Moravvej, Hamideh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Salvia Medical Sciences Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343984/
https://www.ncbi.nlm.nih.gov/pubmed/34466507
http://dx.doi.org/10.31661/gmj.v8i0.1407
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author Rostami-Nejad, Mohammad
vafaee, Reza
Ehsani-Ardakani, Mohammad Javad
Aghamohammadi, Nika
Keramatinia, Aliasghar
Abdi, Saeed
Moravvej, Hamideh
author_facet Rostami-Nejad, Mohammad
vafaee, Reza
Ehsani-Ardakani, Mohammad Javad
Aghamohammadi, Nika
Keramatinia, Aliasghar
Abdi, Saeed
Moravvej, Hamideh
author_sort Rostami-Nejad, Mohammad
collection PubMed
description BACKGROUND: Celiac disease (CD) is an immunological intestinal disorder, which is characterized by response to gluten. In addition to the environmental factors and dysbiosis of the gut microbiota, genetic susceptibility has an important role in the pathogenesis of this multifactorial disorder. Therefore, this study aims to present the crucial involved genes in CD pathogenesis. MATERIALS AND METHODS: In this bioinformatics analysis study, significant differentially expressed genes of intraepithelial lymphocytes (IELs) samples of celiac patients versus normal patients from Gene Expression Omnibus (GEO) database were screened via the protein-protein interaction (PPI) network. The critical nodes based on degree values, betweenness centrality, and fold changes were determined and enriched by ClueGO to find relative biological terms. RESULTS: According to the network analysis, five central nodes including IL2, PIK3CA, PRDM10, AKT1, and SRC and eight significant differentially expressed genes (DEGs) were determined as the critical genes related to CD. Also, CD4+, CD25+, alpha-beta regulatory T cell differentiation are identified as prominent biological terms in the celiac disease patients. CONCLUSION: There is a possible biomarker panel related to CD that can be used as a therapeutic or diagnostic tool to manage the disease.
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spelling pubmed-83439842021-08-30 The Screening of Critical Related Genes in Celiac Disease Based on Intraepithelial Lymphocytes Investigation: A Bioinformatics Analysis Rostami-Nejad, Mohammad vafaee, Reza Ehsani-Ardakani, Mohammad Javad Aghamohammadi, Nika Keramatinia, Aliasghar Abdi, Saeed Moravvej, Hamideh Galen Med J Original Article BACKGROUND: Celiac disease (CD) is an immunological intestinal disorder, which is characterized by response to gluten. In addition to the environmental factors and dysbiosis of the gut microbiota, genetic susceptibility has an important role in the pathogenesis of this multifactorial disorder. Therefore, this study aims to present the crucial involved genes in CD pathogenesis. MATERIALS AND METHODS: In this bioinformatics analysis study, significant differentially expressed genes of intraepithelial lymphocytes (IELs) samples of celiac patients versus normal patients from Gene Expression Omnibus (GEO) database were screened via the protein-protein interaction (PPI) network. The critical nodes based on degree values, betweenness centrality, and fold changes were determined and enriched by ClueGO to find relative biological terms. RESULTS: According to the network analysis, five central nodes including IL2, PIK3CA, PRDM10, AKT1, and SRC and eight significant differentially expressed genes (DEGs) were determined as the critical genes related to CD. Also, CD4+, CD25+, alpha-beta regulatory T cell differentiation are identified as prominent biological terms in the celiac disease patients. CONCLUSION: There is a possible biomarker panel related to CD that can be used as a therapeutic or diagnostic tool to manage the disease. Salvia Medical Sciences Ltd 2019-08-14 /pmc/articles/PMC8343984/ /pubmed/34466507 http://dx.doi.org/10.31661/gmj.v8i0.1407 Text en Copyright© 2019, Galen Medical Journal. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) )
spellingShingle Original Article
Rostami-Nejad, Mohammad
vafaee, Reza
Ehsani-Ardakani, Mohammad Javad
Aghamohammadi, Nika
Keramatinia, Aliasghar
Abdi, Saeed
Moravvej, Hamideh
The Screening of Critical Related Genes in Celiac Disease Based on Intraepithelial Lymphocytes Investigation: A Bioinformatics Analysis
title The Screening of Critical Related Genes in Celiac Disease Based on Intraepithelial Lymphocytes Investigation: A Bioinformatics Analysis
title_full The Screening of Critical Related Genes in Celiac Disease Based on Intraepithelial Lymphocytes Investigation: A Bioinformatics Analysis
title_fullStr The Screening of Critical Related Genes in Celiac Disease Based on Intraepithelial Lymphocytes Investigation: A Bioinformatics Analysis
title_full_unstemmed The Screening of Critical Related Genes in Celiac Disease Based on Intraepithelial Lymphocytes Investigation: A Bioinformatics Analysis
title_short The Screening of Critical Related Genes in Celiac Disease Based on Intraepithelial Lymphocytes Investigation: A Bioinformatics Analysis
title_sort screening of critical related genes in celiac disease based on intraepithelial lymphocytes investigation: a bioinformatics analysis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343984/
https://www.ncbi.nlm.nih.gov/pubmed/34466507
http://dx.doi.org/10.31661/gmj.v8i0.1407
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