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Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway
BACKGROUND: Hepatocellular carcinoma is the most common type of liver cancer which arises from the main cells in the liver. We address many studies investigating anti-cancer role of hypericin, however the proposing corresponding molecular pathway seems to be still a debate. Therefore, the present st...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Salvia Medical Sciences Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344050/ https://www.ncbi.nlm.nih.gov/pubmed/34466606 http://dx.doi.org/10.31661/gmj.v9i0.1896 |
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author | Olya, Maedeh Zaferani Arani, Hamid Shekarriz, Amirhossein Zabolian, Amirhossein Zare Marzouni, Hadi Aryan, Hoda Hoseinian, Mohammad Javidi, Mohammad Amin Atashi, Hesam Adin |
author_facet | Olya, Maedeh Zaferani Arani, Hamid Shekarriz, Amirhossein Zabolian, Amirhossein Zare Marzouni, Hadi Aryan, Hoda Hoseinian, Mohammad Javidi, Mohammad Amin Atashi, Hesam Adin |
author_sort | Olya, Maedeh |
collection | PubMed |
description | BACKGROUND: Hepatocellular carcinoma is the most common type of liver cancer which arises from the main cells in the liver. We address many studies investigating anti-cancer role of hypericin, however the proposing corresponding molecular pathway seems to be still a debate. Therefore, the present study aimed to evaluate the apoptotic effect of hypericin on the Huh7 as the liver cancer cell line and its relation with the gate keeper gene P53. MATERIALS AND METHODS: In this study, the Huh7 cell line and fibroblast cells (as control group) were treated with different concentrations of hypericin for 24 and 48 hours. Detection of cell death was performed by MTT assay and flow cytometry. The expression of bax, bcl2 and p53 mRNAs was evaluated by Real-time PCR. Also, Immunocytochemistry (ICC) analysis was used for further evaluation of P53expression. RESULTS: The results showed that hypericin has a dose-dependent cytotoxic effect on the Huh7 cell line, with no or marginal effect on fibroblastic cells. According to flow cytometry results, about 53%cells underwent apoptosis after exposure to LD50 of hypericin for 24 hours. Real-time PCR data demonstrated that the pro-apoptotic genes Bax and P53 expression level increased. Expectedly ICC results confirmed the up-regulation of P53 proteins in treated samples. CONCLUSION: Our results indicate the cytotoxicity of hypericin on Huh7 cells by affecting the expression of the gate keeper gene P53; furthermore it is suggested that this herb can be utilized simultaneously with modalities targeting P53 up-regulation or related molecular pathways. |
format | Online Article Text |
id | pubmed-8344050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Salvia Medical Sciences Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-83440502021-08-30 Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway Olya, Maedeh Zaferani Arani, Hamid Shekarriz, Amirhossein Zabolian, Amirhossein Zare Marzouni, Hadi Aryan, Hoda Hoseinian, Mohammad Javidi, Mohammad Amin Atashi, Hesam Adin Galen Med J Original Article BACKGROUND: Hepatocellular carcinoma is the most common type of liver cancer which arises from the main cells in the liver. We address many studies investigating anti-cancer role of hypericin, however the proposing corresponding molecular pathway seems to be still a debate. Therefore, the present study aimed to evaluate the apoptotic effect of hypericin on the Huh7 as the liver cancer cell line and its relation with the gate keeper gene P53. MATERIALS AND METHODS: In this study, the Huh7 cell line and fibroblast cells (as control group) were treated with different concentrations of hypericin for 24 and 48 hours. Detection of cell death was performed by MTT assay and flow cytometry. The expression of bax, bcl2 and p53 mRNAs was evaluated by Real-time PCR. Also, Immunocytochemistry (ICC) analysis was used for further evaluation of P53expression. RESULTS: The results showed that hypericin has a dose-dependent cytotoxic effect on the Huh7 cell line, with no or marginal effect on fibroblastic cells. According to flow cytometry results, about 53%cells underwent apoptosis after exposure to LD50 of hypericin for 24 hours. Real-time PCR data demonstrated that the pro-apoptotic genes Bax and P53 expression level increased. Expectedly ICC results confirmed the up-regulation of P53 proteins in treated samples. CONCLUSION: Our results indicate the cytotoxicity of hypericin on Huh7 cells by affecting the expression of the gate keeper gene P53; furthermore it is suggested that this herb can be utilized simultaneously with modalities targeting P53 up-regulation or related molecular pathways. Salvia Medical Sciences Ltd 2020-12-28 /pmc/articles/PMC8344050/ /pubmed/34466606 http://dx.doi.org/10.31661/gmj.v9i0.1896 Text en Copyright© 2020, Galen Medical Journal. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ) |
spellingShingle | Original Article Olya, Maedeh Zaferani Arani, Hamid Shekarriz, Amirhossein Zabolian, Amirhossein Zare Marzouni, Hadi Aryan, Hoda Hoseinian, Mohammad Javidi, Mohammad Amin Atashi, Hesam Adin Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway |
title | Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway |
title_full | Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway |
title_fullStr | Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway |
title_full_unstemmed | Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway |
title_short | Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway |
title_sort | hypericin exerts detrimental effect on huh-7 as a delegacy of hepatocellular carcinoma: a p53 dependent pathway |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344050/ https://www.ncbi.nlm.nih.gov/pubmed/34466606 http://dx.doi.org/10.31661/gmj.v9i0.1896 |
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