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Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway

BACKGROUND: Hepatocellular carcinoma is the most common type of liver cancer which arises from the main cells in the liver. We address many studies investigating anti-cancer role of hypericin, however the proposing corresponding molecular pathway seems to be still a debate. Therefore, the present st...

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Autores principales: Olya, Maedeh, Zaferani Arani, Hamid, Shekarriz, Amirhossein, Zabolian, Amirhossein, Zare Marzouni, Hadi, Aryan, Hoda, Hoseinian, Mohammad, Javidi, Mohammad Amin, Atashi, Hesam Adin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Salvia Medical Sciences Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344050/
https://www.ncbi.nlm.nih.gov/pubmed/34466606
http://dx.doi.org/10.31661/gmj.v9i0.1896
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author Olya, Maedeh
Zaferani Arani, Hamid
Shekarriz, Amirhossein
Zabolian, Amirhossein
Zare Marzouni, Hadi
Aryan, Hoda
Hoseinian, Mohammad
Javidi, Mohammad Amin
Atashi, Hesam Adin
author_facet Olya, Maedeh
Zaferani Arani, Hamid
Shekarriz, Amirhossein
Zabolian, Amirhossein
Zare Marzouni, Hadi
Aryan, Hoda
Hoseinian, Mohammad
Javidi, Mohammad Amin
Atashi, Hesam Adin
author_sort Olya, Maedeh
collection PubMed
description BACKGROUND: Hepatocellular carcinoma is the most common type of liver cancer which arises from the main cells in the liver. We address many studies investigating anti-cancer role of hypericin, however the proposing corresponding molecular pathway seems to be still a debate. Therefore, the present study aimed to evaluate the apoptotic effect of hypericin on the Huh7 as the liver cancer cell line and its relation with the gate keeper gene P53. MATERIALS AND METHODS: In this study, the Huh7 cell line and fibroblast cells (as control group) were treated with different concentrations of hypericin for 24 and 48 hours. Detection of cell death was performed by MTT assay and flow cytometry. The expression of bax, bcl2 and p53 mRNAs was evaluated by Real-time PCR. Also, Immunocytochemistry (ICC) analysis was used for further evaluation of P53expression. RESULTS: The results showed that hypericin has a dose-dependent cytotoxic effect on the Huh7 cell line, with no or marginal effect on fibroblastic cells. According to flow cytometry results, about 53%cells underwent apoptosis after exposure to LD50 of hypericin for 24 hours. Real-time PCR data demonstrated that the pro-apoptotic genes Bax and P53 expression level increased. Expectedly ICC results confirmed the up-regulation of P53 proteins in treated samples. CONCLUSION: Our results indicate the cytotoxicity of hypericin on Huh7 cells by affecting the expression of the gate keeper gene P53; furthermore it is suggested that this herb can be utilized simultaneously with modalities targeting P53 up-regulation or related molecular pathways.
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spelling pubmed-83440502021-08-30 Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway Olya, Maedeh Zaferani Arani, Hamid Shekarriz, Amirhossein Zabolian, Amirhossein Zare Marzouni, Hadi Aryan, Hoda Hoseinian, Mohammad Javidi, Mohammad Amin Atashi, Hesam Adin Galen Med J Original Article BACKGROUND: Hepatocellular carcinoma is the most common type of liver cancer which arises from the main cells in the liver. We address many studies investigating anti-cancer role of hypericin, however the proposing corresponding molecular pathway seems to be still a debate. Therefore, the present study aimed to evaluate the apoptotic effect of hypericin on the Huh7 as the liver cancer cell line and its relation with the gate keeper gene P53. MATERIALS AND METHODS: In this study, the Huh7 cell line and fibroblast cells (as control group) were treated with different concentrations of hypericin for 24 and 48 hours. Detection of cell death was performed by MTT assay and flow cytometry. The expression of bax, bcl2 and p53 mRNAs was evaluated by Real-time PCR. Also, Immunocytochemistry (ICC) analysis was used for further evaluation of P53expression. RESULTS: The results showed that hypericin has a dose-dependent cytotoxic effect on the Huh7 cell line, with no or marginal effect on fibroblastic cells. According to flow cytometry results, about 53%cells underwent apoptosis after exposure to LD50 of hypericin for 24 hours. Real-time PCR data demonstrated that the pro-apoptotic genes Bax and P53 expression level increased. Expectedly ICC results confirmed the up-regulation of P53 proteins in treated samples. CONCLUSION: Our results indicate the cytotoxicity of hypericin on Huh7 cells by affecting the expression of the gate keeper gene P53; furthermore it is suggested that this herb can be utilized simultaneously with modalities targeting P53 up-regulation or related molecular pathways. Salvia Medical Sciences Ltd 2020-12-28 /pmc/articles/PMC8344050/ /pubmed/34466606 http://dx.doi.org/10.31661/gmj.v9i0.1896 Text en Copyright© 2020, Galen Medical Journal. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) )
spellingShingle Original Article
Olya, Maedeh
Zaferani Arani, Hamid
Shekarriz, Amirhossein
Zabolian, Amirhossein
Zare Marzouni, Hadi
Aryan, Hoda
Hoseinian, Mohammad
Javidi, Mohammad Amin
Atashi, Hesam Adin
Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway
title Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway
title_full Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway
title_fullStr Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway
title_full_unstemmed Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway
title_short Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway
title_sort hypericin exerts detrimental effect on huh-7 as a delegacy of hepatocellular carcinoma: a p53 dependent pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344050/
https://www.ncbi.nlm.nih.gov/pubmed/34466606
http://dx.doi.org/10.31661/gmj.v9i0.1896
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