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Reduced Levels of miR–28 and miR–200a Act as Predictor Biomarkers of Aggressive Clinicopathological Characteristics in Gastric Cancer Patients

BACKGROUND: MicroRNAs (miRNAs) play critical roles in different pathological processes including cancer development and progression. To find novel molecular diagnostic and prognostic markers and promising therapeutic tools for gastric cancer (GC), we aimed to investigate the relationship of the expr...

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Autores principales: Jeddi, Farhad, Alipour, Shahriar, Najafzadeh, Nowruz, Dadashpour, Mehdi, Pouremamali, Farhad, Sadeghi, Mohammad Reza, Samadi, Nasser, Soozangar, Narges, Khamaneh, Amir Mahdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Salvia Medical Sciences Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344053/
https://www.ncbi.nlm.nih.gov/pubmed/34466494
http://dx.doi.org/10.31661/gmj.v8i0.1329
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author Jeddi, Farhad
Alipour, Shahriar
Najafzadeh, Nowruz
Dadashpour, Mehdi
Pouremamali, Farhad
Sadeghi, Mohammad Reza
Samadi, Nasser
Soozangar, Narges
Khamaneh, Amir Mahdi
author_facet Jeddi, Farhad
Alipour, Shahriar
Najafzadeh, Nowruz
Dadashpour, Mehdi
Pouremamali, Farhad
Sadeghi, Mohammad Reza
Samadi, Nasser
Soozangar, Narges
Khamaneh, Amir Mahdi
author_sort Jeddi, Farhad
collection PubMed
description BACKGROUND: MicroRNAs (miRNAs) play critical roles in different pathological processes including cancer development and progression. To find novel molecular diagnostic and prognostic markers and promising therapeutic tools for gastric cancer (GC), we aimed to investigate the relationship of the expression levels of miR–28–5p or miR–200a–3p with the clinicopathological criteria and to explore their impacts on the progression of human GC. MATERIALS AND METHODS: Quantitative RT–PCR was performed to analyze miR–28 and miR–200a expression in 60 GC and 60 non–GC tissue samples. RESULT: Our results revealed that the expressions of miR–200a and miR–28 were significantly downregulated in GC in comparison with non– GC tissues. Tumors with low miR–28 expression had larger tumor size, more advanced histological grade, and a higher incidence of lymph node and distal metastasis than the tumors with high miR–28 expressions. Furthermore, receiver operating characteristic (ROC) analyses demonstrate that the expression of miR–28 is a predictive biomarker allows predicting the histological grade, tumor size, and occurrence of nodal and distal metastases. We also found a significant inverse association between miR–200a expression and the rate of lymph node metastasis (p = 0.010, r = –0.334). CONCLUSION: Our findings suggest that the miR–28 and miR–200a have tumor–suppressor functions and may be considered as potential biomarkers for gastric cancer diagnosis and prognosis.
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spelling pubmed-83440532021-08-30 Reduced Levels of miR–28 and miR–200a Act as Predictor Biomarkers of Aggressive Clinicopathological Characteristics in Gastric Cancer Patients Jeddi, Farhad Alipour, Shahriar Najafzadeh, Nowruz Dadashpour, Mehdi Pouremamali, Farhad Sadeghi, Mohammad Reza Samadi, Nasser Soozangar, Narges Khamaneh, Amir Mahdi Galen Med J Original Article BACKGROUND: MicroRNAs (miRNAs) play critical roles in different pathological processes including cancer development and progression. To find novel molecular diagnostic and prognostic markers and promising therapeutic tools for gastric cancer (GC), we aimed to investigate the relationship of the expression levels of miR–28–5p or miR–200a–3p with the clinicopathological criteria and to explore their impacts on the progression of human GC. MATERIALS AND METHODS: Quantitative RT–PCR was performed to analyze miR–28 and miR–200a expression in 60 GC and 60 non–GC tissue samples. RESULT: Our results revealed that the expressions of miR–200a and miR–28 were significantly downregulated in GC in comparison with non– GC tissues. Tumors with low miR–28 expression had larger tumor size, more advanced histological grade, and a higher incidence of lymph node and distal metastasis than the tumors with high miR–28 expressions. Furthermore, receiver operating characteristic (ROC) analyses demonstrate that the expression of miR–28 is a predictive biomarker allows predicting the histological grade, tumor size, and occurrence of nodal and distal metastases. We also found a significant inverse association between miR–200a expression and the rate of lymph node metastasis (p = 0.010, r = –0.334). CONCLUSION: Our findings suggest that the miR–28 and miR–200a have tumor–suppressor functions and may be considered as potential biomarkers for gastric cancer diagnosis and prognosis. Salvia Medical Sciences Ltd 2019-01-25 /pmc/articles/PMC8344053/ /pubmed/34466494 http://dx.doi.org/10.31661/gmj.v8i0.1329 Text en Copyright© 2019, Galen Medical Journal. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) )
spellingShingle Original Article
Jeddi, Farhad
Alipour, Shahriar
Najafzadeh, Nowruz
Dadashpour, Mehdi
Pouremamali, Farhad
Sadeghi, Mohammad Reza
Samadi, Nasser
Soozangar, Narges
Khamaneh, Amir Mahdi
Reduced Levels of miR–28 and miR–200a Act as Predictor Biomarkers of Aggressive Clinicopathological Characteristics in Gastric Cancer Patients
title Reduced Levels of miR–28 and miR–200a Act as Predictor Biomarkers of Aggressive Clinicopathological Characteristics in Gastric Cancer Patients
title_full Reduced Levels of miR–28 and miR–200a Act as Predictor Biomarkers of Aggressive Clinicopathological Characteristics in Gastric Cancer Patients
title_fullStr Reduced Levels of miR–28 and miR–200a Act as Predictor Biomarkers of Aggressive Clinicopathological Characteristics in Gastric Cancer Patients
title_full_unstemmed Reduced Levels of miR–28 and miR–200a Act as Predictor Biomarkers of Aggressive Clinicopathological Characteristics in Gastric Cancer Patients
title_short Reduced Levels of miR–28 and miR–200a Act as Predictor Biomarkers of Aggressive Clinicopathological Characteristics in Gastric Cancer Patients
title_sort reduced levels of mir–28 and mir–200a act as predictor biomarkers of aggressive clinicopathological characteristics in gastric cancer patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8344053/
https://www.ncbi.nlm.nih.gov/pubmed/34466494
http://dx.doi.org/10.31661/gmj.v8i0.1329
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