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TB Antigen-Stimulated CXCR3 Ligand Assay for Diagnosis of Tuberculous Lymphadenitis

The diagnosis of tuberculous lymphadenitis (TB-LAP) is challenging. We evaluated the role of blood CXC chemokine receptor 3 (CXCR3) ligands in its diagnosis. A total of 65 lymphadenopathy patients were enrolled and lymph node sampling was performed. We also recruited 113 control subjects, consisting...

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Autores principales: Chung, Wou-Young, Lee, Keu-Sung, Park, Joo-Hun, Jung, Yun-Jung, Sheen, Seung-Soo, Park, Ji-Eun, Sun, Joo-Sung, Ko, Young-Hwa, Park, Kwang-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345678/
https://www.ncbi.nlm.nih.gov/pubmed/34360313
http://dx.doi.org/10.3390/ijerph18158020
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author Chung, Wou-Young
Lee, Keu-Sung
Park, Joo-Hun
Jung, Yun-Jung
Sheen, Seung-Soo
Park, Ji-Eun
Sun, Joo-Sung
Ko, Young-Hwa
Park, Kwang-Joo
author_facet Chung, Wou-Young
Lee, Keu-Sung
Park, Joo-Hun
Jung, Yun-Jung
Sheen, Seung-Soo
Park, Ji-Eun
Sun, Joo-Sung
Ko, Young-Hwa
Park, Kwang-Joo
author_sort Chung, Wou-Young
collection PubMed
description The diagnosis of tuberculous lymphadenitis (TB-LAP) is challenging. We evaluated the role of blood CXC chemokine receptor 3 (CXCR3) ligands in its diagnosis. A total of 65 lymphadenopathy patients were enrolled and lymph node sampling was performed. We also recruited 113 control subjects, consisting of 27 with positive results and 86 with negative results, in the interferon (IFN)-γ release assay (IGRA). In all study subjects, whole-blood samples were collected using the IGRA methodology. After incubation, plasma levels of IFN-γ and two CXCR3 ligands, IFN-inducible T-cell a chemoattractant (I-TAC) and monokine induced by IFN-γ (MIG), were measured using immunoassay. Fifty-three TB-LAP patients were enrolled. TB antigen-stimulated IFN-γ, I-TAC, and MIG levels were all significantly higher in the TB-LAP patients than in the controls and non-TB-LAP patients. The levels of I-TAC and MIG, but not IFN-γ, showed significant differences between the TB-LAP patients and IGRA-positive controls. Area under the receiver operating characteristic curves (AUROCs) of IFN-γ, I-TAC, and MIG were 0.955, 0.958, and 0.959, respectively, for differentiating TB-LAP from control group, and were 0.912, 0.956, and 0.936, respectively, for differentiating TB-LAP from non-TB-LAP. In conclusion, the TB antigen-stimulated MIG and I-TAC could be useful biomarkers in the diagnosis of TB-LAP.
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spelling pubmed-83456782021-08-07 TB Antigen-Stimulated CXCR3 Ligand Assay for Diagnosis of Tuberculous Lymphadenitis Chung, Wou-Young Lee, Keu-Sung Park, Joo-Hun Jung, Yun-Jung Sheen, Seung-Soo Park, Ji-Eun Sun, Joo-Sung Ko, Young-Hwa Park, Kwang-Joo Int J Environ Res Public Health Article The diagnosis of tuberculous lymphadenitis (TB-LAP) is challenging. We evaluated the role of blood CXC chemokine receptor 3 (CXCR3) ligands in its diagnosis. A total of 65 lymphadenopathy patients were enrolled and lymph node sampling was performed. We also recruited 113 control subjects, consisting of 27 with positive results and 86 with negative results, in the interferon (IFN)-γ release assay (IGRA). In all study subjects, whole-blood samples were collected using the IGRA methodology. After incubation, plasma levels of IFN-γ and two CXCR3 ligands, IFN-inducible T-cell a chemoattractant (I-TAC) and monokine induced by IFN-γ (MIG), were measured using immunoassay. Fifty-three TB-LAP patients were enrolled. TB antigen-stimulated IFN-γ, I-TAC, and MIG levels were all significantly higher in the TB-LAP patients than in the controls and non-TB-LAP patients. The levels of I-TAC and MIG, but not IFN-γ, showed significant differences between the TB-LAP patients and IGRA-positive controls. Area under the receiver operating characteristic curves (AUROCs) of IFN-γ, I-TAC, and MIG were 0.955, 0.958, and 0.959, respectively, for differentiating TB-LAP from control group, and were 0.912, 0.956, and 0.936, respectively, for differentiating TB-LAP from non-TB-LAP. In conclusion, the TB antigen-stimulated MIG and I-TAC could be useful biomarkers in the diagnosis of TB-LAP. MDPI 2021-07-29 /pmc/articles/PMC8345678/ /pubmed/34360313 http://dx.doi.org/10.3390/ijerph18158020 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chung, Wou-Young
Lee, Keu-Sung
Park, Joo-Hun
Jung, Yun-Jung
Sheen, Seung-Soo
Park, Ji-Eun
Sun, Joo-Sung
Ko, Young-Hwa
Park, Kwang-Joo
TB Antigen-Stimulated CXCR3 Ligand Assay for Diagnosis of Tuberculous Lymphadenitis
title TB Antigen-Stimulated CXCR3 Ligand Assay for Diagnosis of Tuberculous Lymphadenitis
title_full TB Antigen-Stimulated CXCR3 Ligand Assay for Diagnosis of Tuberculous Lymphadenitis
title_fullStr TB Antigen-Stimulated CXCR3 Ligand Assay for Diagnosis of Tuberculous Lymphadenitis
title_full_unstemmed TB Antigen-Stimulated CXCR3 Ligand Assay for Diagnosis of Tuberculous Lymphadenitis
title_short TB Antigen-Stimulated CXCR3 Ligand Assay for Diagnosis of Tuberculous Lymphadenitis
title_sort tb antigen-stimulated cxcr3 ligand assay for diagnosis of tuberculous lymphadenitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345678/
https://www.ncbi.nlm.nih.gov/pubmed/34360313
http://dx.doi.org/10.3390/ijerph18158020
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