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Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network
Surveillance and detection of polioviruses (PV) remain crucial to monitoring eradication progress. Intratypic differentiation (ITD) using the real-time RT-PCR kit is key to the surveillance workflow, where viruses are screened after cell culture isolation before a subset are verified by sequencing....
Autores principales: | Sun, Hong, Harrington, Chelsea, Gerloff, Nancy, Mandelbaum, Mark, Jeffries-Miles, Stacey, Apostol, Lea Necitas G., Valencia, Ma. Anne-Lesley D., Shaukat, Shahzad, Angez, Mehar, Sharma, Deepa K., Nalavade, Uma P., Pawar, Shailesh D., Pukuta Simbu, Elisabeth, Andriamamonjy, Seta, Razafindratsimandresy, Richter, Vega, Everardo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345876/ https://www.ncbi.nlm.nih.gov/pubmed/34358268 http://dx.doi.org/10.1371/journal.pone.0255795 |
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