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Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages

Macrophages play a critical role in the inflammatory response to environmental triggers, such as lipopolysaccharide (LPS). Inflammatory signaling through macrophages and the innate immune system are increasingly recognized as important contributors to multiple acute and chronic disease processes. Ni...

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Autores principales: Lee, Sang Min, Son, Kyung-No, Shah, Dhara, Ali, Marwan, Balasubramaniam, Arun, Shukla, Deepak, Aakalu, Vinay Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345949/
https://www.ncbi.nlm.nih.gov/pubmed/34360629
http://dx.doi.org/10.3390/ijms22157856
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author Lee, Sang Min
Son, Kyung-No
Shah, Dhara
Ali, Marwan
Balasubramaniam, Arun
Shukla, Deepak
Aakalu, Vinay Kumar
author_facet Lee, Sang Min
Son, Kyung-No
Shah, Dhara
Ali, Marwan
Balasubramaniam, Arun
Shukla, Deepak
Aakalu, Vinay Kumar
author_sort Lee, Sang Min
collection PubMed
description Macrophages play a critical role in the inflammatory response to environmental triggers, such as lipopolysaccharide (LPS). Inflammatory signaling through macrophages and the innate immune system are increasingly recognized as important contributors to multiple acute and chronic disease processes. Nitric oxide (NO) is a free radical that plays an important role in immune and inflammatory responses as an important intercellular messenger. In addition, NO has an important role in inflammatory responses in mucosal environments such as the ocular surface. Histatin peptides are well-established antimicrobial and wound healing agents. These peptides are important in multiple biological systems, playing roles in responses to the environment and immunomodulation. Given the importance of macrophages in responses to environmental triggers and pathogens, we investigated the effect of histatin-1 (Hst1) on LPS-induced inflammatory responses and the underlying molecular mechanisms in RAW264.7 (RAW) macrophages. LPS-induced inflammatory signaling, NO production and cytokine production in macrophages were tested in response to treatment with Hst1. Hst1 application significantly reduced LPS-induced NO production, inflammatory cytokine production, and inflammatory signaling through the JNK and NF-kB pathways in RAW cells. These results demonstrate that Hst1 can inhibit LPS-induced inflammatory mediator production and MAPK signaling pathways in macrophages.
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spelling pubmed-83459492021-08-07 Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages Lee, Sang Min Son, Kyung-No Shah, Dhara Ali, Marwan Balasubramaniam, Arun Shukla, Deepak Aakalu, Vinay Kumar Int J Mol Sci Article Macrophages play a critical role in the inflammatory response to environmental triggers, such as lipopolysaccharide (LPS). Inflammatory signaling through macrophages and the innate immune system are increasingly recognized as important contributors to multiple acute and chronic disease processes. Nitric oxide (NO) is a free radical that plays an important role in immune and inflammatory responses as an important intercellular messenger. In addition, NO has an important role in inflammatory responses in mucosal environments such as the ocular surface. Histatin peptides are well-established antimicrobial and wound healing agents. These peptides are important in multiple biological systems, playing roles in responses to the environment and immunomodulation. Given the importance of macrophages in responses to environmental triggers and pathogens, we investigated the effect of histatin-1 (Hst1) on LPS-induced inflammatory responses and the underlying molecular mechanisms in RAW264.7 (RAW) macrophages. LPS-induced inflammatory signaling, NO production and cytokine production in macrophages were tested in response to treatment with Hst1. Hst1 application significantly reduced LPS-induced NO production, inflammatory cytokine production, and inflammatory signaling through the JNK and NF-kB pathways in RAW cells. These results demonstrate that Hst1 can inhibit LPS-induced inflammatory mediator production and MAPK signaling pathways in macrophages. MDPI 2021-07-23 /pmc/articles/PMC8345949/ /pubmed/34360629 http://dx.doi.org/10.3390/ijms22157856 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Sang Min
Son, Kyung-No
Shah, Dhara
Ali, Marwan
Balasubramaniam, Arun
Shukla, Deepak
Aakalu, Vinay Kumar
Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages
title Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages
title_full Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages
title_fullStr Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages
title_full_unstemmed Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages
title_short Histatin-1 Attenuates LPS-Induced Inflammatory Signaling in RAW264.7 Macrophages
title_sort histatin-1 attenuates lps-induced inflammatory signaling in raw264.7 macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345949/
https://www.ncbi.nlm.nih.gov/pubmed/34360629
http://dx.doi.org/10.3390/ijms22157856
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