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Complexome Profiling: Assembly and Remodeling of Protein Complexes
Many proteins have been found to operate in a complex with various biomolecules such as proteins, nucleic acids, carbohydrates, or lipids. Protein complexes can be transient, stable or dynamic and their association is controlled under variable cellular conditions. Complexome profiling is a recently...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346016/ https://www.ncbi.nlm.nih.gov/pubmed/34360575 http://dx.doi.org/10.3390/ijms22157809 |
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author | Wittig, Ilka Malacarne, Pedro Felipe |
author_facet | Wittig, Ilka Malacarne, Pedro Felipe |
author_sort | Wittig, Ilka |
collection | PubMed |
description | Many proteins have been found to operate in a complex with various biomolecules such as proteins, nucleic acids, carbohydrates, or lipids. Protein complexes can be transient, stable or dynamic and their association is controlled under variable cellular conditions. Complexome profiling is a recently developed mass spectrometry-based method that combines mild separation techniques, native gel electrophoresis, and density gradient centrifugation with quantitative mass spectrometry to generate inventories of protein assemblies within a cell or subcellular fraction. This review summarizes applications of complexome profiling with respect to assembly ranging from single subunits to large macromolecular complexes, as well as their stability, and remodeling in health and disease. |
format | Online Article Text |
id | pubmed-8346016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83460162021-08-07 Complexome Profiling: Assembly and Remodeling of Protein Complexes Wittig, Ilka Malacarne, Pedro Felipe Int J Mol Sci Review Many proteins have been found to operate in a complex with various biomolecules such as proteins, nucleic acids, carbohydrates, or lipids. Protein complexes can be transient, stable or dynamic and their association is controlled under variable cellular conditions. Complexome profiling is a recently developed mass spectrometry-based method that combines mild separation techniques, native gel electrophoresis, and density gradient centrifugation with quantitative mass spectrometry to generate inventories of protein assemblies within a cell or subcellular fraction. This review summarizes applications of complexome profiling with respect to assembly ranging from single subunits to large macromolecular complexes, as well as their stability, and remodeling in health and disease. MDPI 2021-07-21 /pmc/articles/PMC8346016/ /pubmed/34360575 http://dx.doi.org/10.3390/ijms22157809 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Wittig, Ilka Malacarne, Pedro Felipe Complexome Profiling: Assembly and Remodeling of Protein Complexes |
title | Complexome Profiling: Assembly and Remodeling of Protein Complexes |
title_full | Complexome Profiling: Assembly and Remodeling of Protein Complexes |
title_fullStr | Complexome Profiling: Assembly and Remodeling of Protein Complexes |
title_full_unstemmed | Complexome Profiling: Assembly and Remodeling of Protein Complexes |
title_short | Complexome Profiling: Assembly and Remodeling of Protein Complexes |
title_sort | complexome profiling: assembly and remodeling of protein complexes |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346016/ https://www.ncbi.nlm.nih.gov/pubmed/34360575 http://dx.doi.org/10.3390/ijms22157809 |
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