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Bone Status in a Mouse Model of Experimental Autoimmune-Orchitis
Investigations in male patients with fertility disorders revealed a greater risk of osteoporosis. The rodent model of experimental autoimmune-orchitis (EAO) was established to analyze the underlying mechanisms of male infertility and causes of reduced testosterone concentration. Hence, we investigat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346031/ https://www.ncbi.nlm.nih.gov/pubmed/34360623 http://dx.doi.org/10.3390/ijms22157858 |
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author | Hemm, Fabian Fijak, Monika Belikan, Jan Kampschulte, Marian El Khassawna, Thaqif Pilatz, Adrian Heiss, Christian Lips, Katrin Susanne |
author_facet | Hemm, Fabian Fijak, Monika Belikan, Jan Kampschulte, Marian El Khassawna, Thaqif Pilatz, Adrian Heiss, Christian Lips, Katrin Susanne |
author_sort | Hemm, Fabian |
collection | PubMed |
description | Investigations in male patients with fertility disorders revealed a greater risk of osteoporosis. The rodent model of experimental autoimmune-orchitis (EAO) was established to analyze the underlying mechanisms of male infertility and causes of reduced testosterone concentration. Hence, we investigated the impact of testicular dysfunction in EAO on bone status. Male mice were immunized with testicular homogenate in adjuvant to induce EAO (n = 5). Age-matched mice were treated with adjuvant alone (adjuvant, n = 6) or remained untreated (control, n = 7). Fifty days after the first immunization specimens were harvested. Real-time reverse transcription-PCR indicated decreased bone metabolism by alkaline phosphatase and Cathepsin K as well as remodeling of cell-contacts by Connexin-43. Micro computed tomography demonstrated a loss of bone mass and mineralization. These findings were supported by histomorphometric results. Additionally, biomechanical properties of femora in a three-point bending test were significantly altered. In summary, the present study illustrates the induction of osteoporosis in the investigated mouse model. However, results suggest that the major effects on bone status were mainly caused by the complete Freund’s adjuvant rather than the autoimmune-orchitis itself. Therefore, the benefit of the EAO model to transfer laboratory findings regarding bone metabolism in context with orchitis into a clinical application is limited. |
format | Online Article Text |
id | pubmed-8346031 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83460312021-08-07 Bone Status in a Mouse Model of Experimental Autoimmune-Orchitis Hemm, Fabian Fijak, Monika Belikan, Jan Kampschulte, Marian El Khassawna, Thaqif Pilatz, Adrian Heiss, Christian Lips, Katrin Susanne Int J Mol Sci Article Investigations in male patients with fertility disorders revealed a greater risk of osteoporosis. The rodent model of experimental autoimmune-orchitis (EAO) was established to analyze the underlying mechanisms of male infertility and causes of reduced testosterone concentration. Hence, we investigated the impact of testicular dysfunction in EAO on bone status. Male mice were immunized with testicular homogenate in adjuvant to induce EAO (n = 5). Age-matched mice were treated with adjuvant alone (adjuvant, n = 6) or remained untreated (control, n = 7). Fifty days after the first immunization specimens were harvested. Real-time reverse transcription-PCR indicated decreased bone metabolism by alkaline phosphatase and Cathepsin K as well as remodeling of cell-contacts by Connexin-43. Micro computed tomography demonstrated a loss of bone mass and mineralization. These findings were supported by histomorphometric results. Additionally, biomechanical properties of femora in a three-point bending test were significantly altered. In summary, the present study illustrates the induction of osteoporosis in the investigated mouse model. However, results suggest that the major effects on bone status were mainly caused by the complete Freund’s adjuvant rather than the autoimmune-orchitis itself. Therefore, the benefit of the EAO model to transfer laboratory findings regarding bone metabolism in context with orchitis into a clinical application is limited. MDPI 2021-07-23 /pmc/articles/PMC8346031/ /pubmed/34360623 http://dx.doi.org/10.3390/ijms22157858 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hemm, Fabian Fijak, Monika Belikan, Jan Kampschulte, Marian El Khassawna, Thaqif Pilatz, Adrian Heiss, Christian Lips, Katrin Susanne Bone Status in a Mouse Model of Experimental Autoimmune-Orchitis |
title | Bone Status in a Mouse Model of Experimental Autoimmune-Orchitis |
title_full | Bone Status in a Mouse Model of Experimental Autoimmune-Orchitis |
title_fullStr | Bone Status in a Mouse Model of Experimental Autoimmune-Orchitis |
title_full_unstemmed | Bone Status in a Mouse Model of Experimental Autoimmune-Orchitis |
title_short | Bone Status in a Mouse Model of Experimental Autoimmune-Orchitis |
title_sort | bone status in a mouse model of experimental autoimmune-orchitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346031/ https://www.ncbi.nlm.nih.gov/pubmed/34360623 http://dx.doi.org/10.3390/ijms22157858 |
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