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Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands of Wistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments

In the present study, we analyzed the activity of several aminopeptidases (angiotensinases) involved in the metabolism of various angiotensin peptides, in pituitary and adrenal glands of untreated Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR) or treated with the antihypertensive drugs...

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Autores principales: Segarra, Ana B., Prieto, Isabel, Banegas, Inmaculada, Martínez-Cañamero, Magdalena, Villarejo, Ana B., Domínguez-Vías, Germán, de Gasparo, Marc, Ramírez-Sánchez, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346081/
https://www.ncbi.nlm.nih.gov/pubmed/34360587
http://dx.doi.org/10.3390/ijms22157823
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author Segarra, Ana B.
Prieto, Isabel
Banegas, Inmaculada
Martínez-Cañamero, Magdalena
Villarejo, Ana B.
Domínguez-Vías, Germán
de Gasparo, Marc
Ramírez-Sánchez, Manuel
author_facet Segarra, Ana B.
Prieto, Isabel
Banegas, Inmaculada
Martínez-Cañamero, Magdalena
Villarejo, Ana B.
Domínguez-Vías, Germán
de Gasparo, Marc
Ramírez-Sánchez, Manuel
author_sort Segarra, Ana B.
collection PubMed
description In the present study, we analyzed the activity of several aminopeptidases (angiotensinases) involved in the metabolism of various angiotensin peptides, in pituitary and adrenal glands of untreated Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR) or treated with the antihypertensive drugs captopril and propranolol or with the L-Arginine hypertensive analogue L-NG-Nitroarginine Methyl Ester (L-NAME). Intra- and inter-gland correlations between angiotensinase activities were also calculated. Membrane-bound alanyl-, cystinyl-, and glutamyl-aminopeptidase activities were determined fluorometrically using aminoacyl-β-naphthylamide as substrates. Depending on the type of angiotensinase analyzed, the results reflect a complex picture showing substantial differences between glands, strains, and treatments. Alanyl-aminopeptidase responsible for the metabolism of Ang III to Ang IV appears to be the most active angiotensinase in both pituitary and adrenals of WKY and particularly in SHR. Independently of treatment, most positive correlations are observed in the pituitary gland of WKY whereas such positive correlations are predominant in adrenals of SHR. Negative inter-gland correlations were observed in control SHR and L-NAME treated WKY. Positive inter-gland correlations were observed in captopril-treated SHR and propranolol-treated WKY. These results may reflect additional mechanisms for increasing or decreasing systolic blood pressure in WKY or SHR.
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spelling pubmed-83460812021-08-07 Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands of Wistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments Segarra, Ana B. Prieto, Isabel Banegas, Inmaculada Martínez-Cañamero, Magdalena Villarejo, Ana B. Domínguez-Vías, Germán de Gasparo, Marc Ramírez-Sánchez, Manuel Int J Mol Sci Article In the present study, we analyzed the activity of several aminopeptidases (angiotensinases) involved in the metabolism of various angiotensin peptides, in pituitary and adrenal glands of untreated Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR) or treated with the antihypertensive drugs captopril and propranolol or with the L-Arginine hypertensive analogue L-NG-Nitroarginine Methyl Ester (L-NAME). Intra- and inter-gland correlations between angiotensinase activities were also calculated. Membrane-bound alanyl-, cystinyl-, and glutamyl-aminopeptidase activities were determined fluorometrically using aminoacyl-β-naphthylamide as substrates. Depending on the type of angiotensinase analyzed, the results reflect a complex picture showing substantial differences between glands, strains, and treatments. Alanyl-aminopeptidase responsible for the metabolism of Ang III to Ang IV appears to be the most active angiotensinase in both pituitary and adrenals of WKY and particularly in SHR. Independently of treatment, most positive correlations are observed in the pituitary gland of WKY whereas such positive correlations are predominant in adrenals of SHR. Negative inter-gland correlations were observed in control SHR and L-NAME treated WKY. Positive inter-gland correlations were observed in captopril-treated SHR and propranolol-treated WKY. These results may reflect additional mechanisms for increasing or decreasing systolic blood pressure in WKY or SHR. MDPI 2021-07-22 /pmc/articles/PMC8346081/ /pubmed/34360587 http://dx.doi.org/10.3390/ijms22157823 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Segarra, Ana B.
Prieto, Isabel
Banegas, Inmaculada
Martínez-Cañamero, Magdalena
Villarejo, Ana B.
Domínguez-Vías, Germán
de Gasparo, Marc
Ramírez-Sánchez, Manuel
Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands of Wistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments
title Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands of Wistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments
title_full Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands of Wistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments
title_fullStr Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands of Wistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments
title_full_unstemmed Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands of Wistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments
title_short Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands of Wistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments
title_sort interaction between angiotensinase activities in pituitary and adrenal glands of wistar–kyoto and spontaneously hypertensive rats under hypotensive or hypertensive treatments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346081/
https://www.ncbi.nlm.nih.gov/pubmed/34360587
http://dx.doi.org/10.3390/ijms22157823
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