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Insertion of Badnaviral DNA in the Late Blight Resistance Gene (R1a) of Brinjal Eggplant (Solanum melongena)

Endogenous viral elements (EVEs) are widespread in plant genomes. They result from the random integration of viral sequences into host plant genomes by horizontal DNA transfer and have the potential to alter host gene expression. We performed a large-scale search for co-transcripts including caulimo...

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Autores principales: Serfraz, Saad, Sharma, Vikas, Maumus, Florian, Aubriot, Xavier, Geering, Andrew D. W., Teycheney, Pierre-Yves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346255/
https://www.ncbi.nlm.nih.gov/pubmed/34367211
http://dx.doi.org/10.3389/fpls.2021.683681
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author Serfraz, Saad
Sharma, Vikas
Maumus, Florian
Aubriot, Xavier
Geering, Andrew D. W.
Teycheney, Pierre-Yves
author_facet Serfraz, Saad
Sharma, Vikas
Maumus, Florian
Aubriot, Xavier
Geering, Andrew D. W.
Teycheney, Pierre-Yves
author_sort Serfraz, Saad
collection PubMed
description Endogenous viral elements (EVEs) are widespread in plant genomes. They result from the random integration of viral sequences into host plant genomes by horizontal DNA transfer and have the potential to alter host gene expression. We performed a large-scale search for co-transcripts including caulimovirid and plant sequences in 1,678 plant and 230 algal species and characterized 50 co-transcripts in 45 distinct plant species belonging to lycophytes, ferns, gymnosperms and angiosperms. We found that insertion of badnavirus EVEs along with Ty-1 copia mobile elements occurred into a late blight resistance gene (R1) of brinjal eggplant (Solanum melongena) and wild relatives in genus Solanum and disrupted R1 orthologs. EVEs of two previously unreported badnaviruses were identified in the genome of S. melongena, whereas EVEs from an additional novel badnavirus were identified in the genome of S. aethiopicum, the cultivated scarlet eggplant. Insertion of these viruses in the ancestral lineages of the direct wild relatives of the eggplant would have occurred during the last 3 Myr, further supporting the distinctiveness of the group of the eggplant within the giant genus Solanum.
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spelling pubmed-83462552021-08-07 Insertion of Badnaviral DNA in the Late Blight Resistance Gene (R1a) of Brinjal Eggplant (Solanum melongena) Serfraz, Saad Sharma, Vikas Maumus, Florian Aubriot, Xavier Geering, Andrew D. W. Teycheney, Pierre-Yves Front Plant Sci Plant Science Endogenous viral elements (EVEs) are widespread in plant genomes. They result from the random integration of viral sequences into host plant genomes by horizontal DNA transfer and have the potential to alter host gene expression. We performed a large-scale search for co-transcripts including caulimovirid and plant sequences in 1,678 plant and 230 algal species and characterized 50 co-transcripts in 45 distinct plant species belonging to lycophytes, ferns, gymnosperms and angiosperms. We found that insertion of badnavirus EVEs along with Ty-1 copia mobile elements occurred into a late blight resistance gene (R1) of brinjal eggplant (Solanum melongena) and wild relatives in genus Solanum and disrupted R1 orthologs. EVEs of two previously unreported badnaviruses were identified in the genome of S. melongena, whereas EVEs from an additional novel badnavirus were identified in the genome of S. aethiopicum, the cultivated scarlet eggplant. Insertion of these viruses in the ancestral lineages of the direct wild relatives of the eggplant would have occurred during the last 3 Myr, further supporting the distinctiveness of the group of the eggplant within the giant genus Solanum. Frontiers Media S.A. 2021-07-23 /pmc/articles/PMC8346255/ /pubmed/34367211 http://dx.doi.org/10.3389/fpls.2021.683681 Text en Copyright © 2021 Serfraz, Sharma, Maumus, Aubriot, Geering and Teycheney. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Serfraz, Saad
Sharma, Vikas
Maumus, Florian
Aubriot, Xavier
Geering, Andrew D. W.
Teycheney, Pierre-Yves
Insertion of Badnaviral DNA in the Late Blight Resistance Gene (R1a) of Brinjal Eggplant (Solanum melongena)
title Insertion of Badnaviral DNA in the Late Blight Resistance Gene (R1a) of Brinjal Eggplant (Solanum melongena)
title_full Insertion of Badnaviral DNA in the Late Blight Resistance Gene (R1a) of Brinjal Eggplant (Solanum melongena)
title_fullStr Insertion of Badnaviral DNA in the Late Blight Resistance Gene (R1a) of Brinjal Eggplant (Solanum melongena)
title_full_unstemmed Insertion of Badnaviral DNA in the Late Blight Resistance Gene (R1a) of Brinjal Eggplant (Solanum melongena)
title_short Insertion of Badnaviral DNA in the Late Blight Resistance Gene (R1a) of Brinjal Eggplant (Solanum melongena)
title_sort insertion of badnaviral dna in the late blight resistance gene (r1a) of brinjal eggplant (solanum melongena)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346255/
https://www.ncbi.nlm.nih.gov/pubmed/34367211
http://dx.doi.org/10.3389/fpls.2021.683681
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