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Discovery and characterization of H(v)1-type proton channels in reef-building corals
Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence of a gene encoding H(v)1 voltage-dependent, proton-permeable channels in two species of reef-building corals. We performed a characterization of thei...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346283/ https://www.ncbi.nlm.nih.gov/pubmed/34355697 http://dx.doi.org/10.7554/eLife.69248 |
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author | Rangel-Yescas, Gisela Cervantes, Cecilia Cervantes-Rocha, Miguel A Suárez-Delgado, Esteban Banaszak, Anastazia T Maldonado, Ernesto Ramsey, Ian Scott Rosenbaum, Tamara Islas, Leon D |
author_facet | Rangel-Yescas, Gisela Cervantes, Cecilia Cervantes-Rocha, Miguel A Suárez-Delgado, Esteban Banaszak, Anastazia T Maldonado, Ernesto Ramsey, Ian Scott Rosenbaum, Tamara Islas, Leon D |
author_sort | Rangel-Yescas, Gisela |
collection | PubMed |
description | Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence of a gene encoding H(v)1 voltage-dependent, proton-permeable channels in two species of reef-building corals. We performed a characterization of their biophysical properties and found that these channels are fast-activating and modulated by the pH gradient in a distinct manner. The biophysical properties of these novel channels make them interesting model systems. We have also developed an allosteric gating model that provides mechanistic insight into the modulation of voltage-dependence by protons. This work also represents the first functional characterization of any ion channel in scleractinian corals. We discuss the implications of the presence of these channels in the membranes of coral cells in the calcification and pH-regulation processes and possible consequences of ocean acidification related to the function of these channels. |
format | Online Article Text |
id | pubmed-8346283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-83462832021-08-09 Discovery and characterization of H(v)1-type proton channels in reef-building corals Rangel-Yescas, Gisela Cervantes, Cecilia Cervantes-Rocha, Miguel A Suárez-Delgado, Esteban Banaszak, Anastazia T Maldonado, Ernesto Ramsey, Ian Scott Rosenbaum, Tamara Islas, Leon D eLife Structural Biology and Molecular Biophysics Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence of a gene encoding H(v)1 voltage-dependent, proton-permeable channels in two species of reef-building corals. We performed a characterization of their biophysical properties and found that these channels are fast-activating and modulated by the pH gradient in a distinct manner. The biophysical properties of these novel channels make them interesting model systems. We have also developed an allosteric gating model that provides mechanistic insight into the modulation of voltage-dependence by protons. This work also represents the first functional characterization of any ion channel in scleractinian corals. We discuss the implications of the presence of these channels in the membranes of coral cells in the calcification and pH-regulation processes and possible consequences of ocean acidification related to the function of these channels. eLife Sciences Publications, Ltd 2021-08-06 /pmc/articles/PMC8346283/ /pubmed/34355697 http://dx.doi.org/10.7554/eLife.69248 Text en © 2021, Rangel-Yescas et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Structural Biology and Molecular Biophysics Rangel-Yescas, Gisela Cervantes, Cecilia Cervantes-Rocha, Miguel A Suárez-Delgado, Esteban Banaszak, Anastazia T Maldonado, Ernesto Ramsey, Ian Scott Rosenbaum, Tamara Islas, Leon D Discovery and characterization of H(v)1-type proton channels in reef-building corals |
title | Discovery and characterization of H(v)1-type proton channels in reef-building corals |
title_full | Discovery and characterization of H(v)1-type proton channels in reef-building corals |
title_fullStr | Discovery and characterization of H(v)1-type proton channels in reef-building corals |
title_full_unstemmed | Discovery and characterization of H(v)1-type proton channels in reef-building corals |
title_short | Discovery and characterization of H(v)1-type proton channels in reef-building corals |
title_sort | discovery and characterization of h(v)1-type proton channels in reef-building corals |
topic | Structural Biology and Molecular Biophysics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346283/ https://www.ncbi.nlm.nih.gov/pubmed/34355697 http://dx.doi.org/10.7554/eLife.69248 |
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