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Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy

Chromatin condensation is one of the main factors essential for sperm function. Evaluation of chromatin condensation by current methods render the assessed sperm unsuitable for assisted reproduction. We examined the Raman spectra of normal morphology sperm to determine whether a non-invasive confoca...

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Autores principales: Jahmani, M. Y., Hammadeh, M. E., Al Smadi, M. A., Baller, Marko K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346437/
https://www.ncbi.nlm.nih.gov/pubmed/33877640
http://dx.doi.org/10.1007/s43032-021-00494-6
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author Jahmani, M. Y.
Hammadeh, M. E.
Al Smadi, M. A.
Baller, Marko K.
author_facet Jahmani, M. Y.
Hammadeh, M. E.
Al Smadi, M. A.
Baller, Marko K.
author_sort Jahmani, M. Y.
collection PubMed
description Chromatin condensation is one of the main factors essential for sperm function. Evaluation of chromatin condensation by current methods render the assessed sperm unsuitable for assisted reproduction. We examined the Raman spectra of normal morphology sperm to determine whether a non-invasive confocal Raman spectroscopy can detect spectral differences between groups having different levels of chromatin condensation. Semen samples from 85 donors who underwent ICSI were obtained. Chromomycin A3, aniline blue and acridine orange staining were performed to evaluate the protamine deficiency, histone retention and DNA fragmentation respectively. Raman spectra were obtained from 50 normal morphology sperm for each donor. Spectral analysis was performed using home written programs in LabVIEW software and samples were grouped based on chromomycin A3 staining. Raman peaks intensities at 670 cm(-1), 731 cm(-1), 785 cm(-1), 858 cm(-1), 1062 cm(-1), 1098 cm(-1), 1185 cm(-1), 1372 cm(-1), 1424 cm(-1), 1450 cm(-1), 1532 cm(-1), 1618 cm(-1) and 1673 cm(-1) were significantly correlated with at least one of the sperm staining methods. The median intensity of the Raman peaks at 670 cm(-1), 731 cm(-1), 785 cm(-1), 1062 cm(-1), 1098 cm(-1), 1185 cm(-1), 1372 cm(-1), 1424 cm(-1), 1450 cm(-1), 1532 cm(-1), 1618 cm(-1) and 1673 cm(-1) show a significant difference between the CMA3≤41 and CMA3>41groups. The Raman spectroscopic measurements represent a promising diagnostic tool that has the ability to label-free detect sperm with chromatin abnormalities, such as improper chromatin condensation and DNA fragmentation to a certain degree similar to that of the existing staining techniques at the individual cell level.
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spelling pubmed-83464372021-08-20 Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy Jahmani, M. Y. Hammadeh, M. E. Al Smadi, M. A. Baller, Marko K. Reprod Sci Infertility: Original Article Chromatin condensation is one of the main factors essential for sperm function. Evaluation of chromatin condensation by current methods render the assessed sperm unsuitable for assisted reproduction. We examined the Raman spectra of normal morphology sperm to determine whether a non-invasive confocal Raman spectroscopy can detect spectral differences between groups having different levels of chromatin condensation. Semen samples from 85 donors who underwent ICSI were obtained. Chromomycin A3, aniline blue and acridine orange staining were performed to evaluate the protamine deficiency, histone retention and DNA fragmentation respectively. Raman spectra were obtained from 50 normal morphology sperm for each donor. Spectral analysis was performed using home written programs in LabVIEW software and samples were grouped based on chromomycin A3 staining. Raman peaks intensities at 670 cm(-1), 731 cm(-1), 785 cm(-1), 858 cm(-1), 1062 cm(-1), 1098 cm(-1), 1185 cm(-1), 1372 cm(-1), 1424 cm(-1), 1450 cm(-1), 1532 cm(-1), 1618 cm(-1) and 1673 cm(-1) were significantly correlated with at least one of the sperm staining methods. The median intensity of the Raman peaks at 670 cm(-1), 731 cm(-1), 785 cm(-1), 1062 cm(-1), 1098 cm(-1), 1185 cm(-1), 1372 cm(-1), 1424 cm(-1), 1450 cm(-1), 1532 cm(-1), 1618 cm(-1) and 1673 cm(-1) show a significant difference between the CMA3≤41 and CMA3>41groups. The Raman spectroscopic measurements represent a promising diagnostic tool that has the ability to label-free detect sperm with chromatin abnormalities, such as improper chromatin condensation and DNA fragmentation to a certain degree similar to that of the existing staining techniques at the individual cell level. Springer International Publishing 2021-04-20 /pmc/articles/PMC8346437/ /pubmed/33877640 http://dx.doi.org/10.1007/s43032-021-00494-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Infertility: Original Article
Jahmani, M. Y.
Hammadeh, M. E.
Al Smadi, M. A.
Baller, Marko K.
Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy
title Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy
title_full Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy
title_fullStr Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy
title_full_unstemmed Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy
title_short Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy
title_sort label-free evaluation of chromatin condensation in human normal morphology sperm using raman spectroscopy
topic Infertility: Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346437/
https://www.ncbi.nlm.nih.gov/pubmed/33877640
http://dx.doi.org/10.1007/s43032-021-00494-6
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