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Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties

Despite the prevalence of large (>5 cm(2)) articular cartilage defects involving underlying bone, current tissue-engineered therapies only address small defects. Tissue-engineered, anatomically shaped, native-like implants may address the need for off-the-shelf, tissue-repairing therapies for lar...

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Autores principales: Brown, Wendy E., Huang, Brian J., Hu, Jerry C., Athanasiou, Kyriacos A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346478/
https://www.ncbi.nlm.nih.gov/pubmed/34362933
http://dx.doi.org/10.1038/s41536-021-00152-0
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author Brown, Wendy E.
Huang, Brian J.
Hu, Jerry C.
Athanasiou, Kyriacos A.
author_facet Brown, Wendy E.
Huang, Brian J.
Hu, Jerry C.
Athanasiou, Kyriacos A.
author_sort Brown, Wendy E.
collection PubMed
description Despite the prevalence of large (>5 cm(2)) articular cartilage defects involving underlying bone, current tissue-engineered therapies only address small defects. Tissue-engineered, anatomically shaped, native-like implants may address the need for off-the-shelf, tissue-repairing therapies for large cartilage lesions. This study fabricated an osteochondral construct of translationally relevant geometry with robust functional properties. Scaffold-free, self-assembled neocartilage served as the chondral phase, and porous hydroxyapatite served as the osseous phase of the osteochondral constructs. Constructs in the shape and size of an ovine femoral condyle (31 × 14 mm) were assembled at day 4 (early) or day 10 (late) of neocartilage maturation. Early osteochondral assembly increased the interfacial interdigitation depth by 244%, interdigitation frequency by 438%, interfacial shear modulus by 243-fold, and ultimate interfacial shear strength by 4.9-fold, compared to late assembly. Toward the development of a bioprosthesis for the repair of cartilage lesions encompassing up to an entire condylar surface, this study generated a large, anatomically shaped osteochondral construct with robust interfacial mechanical properties and native-like neocartilage interdigitation.
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spelling pubmed-83464782021-08-20 Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties Brown, Wendy E. Huang, Brian J. Hu, Jerry C. Athanasiou, Kyriacos A. NPJ Regen Med Article Despite the prevalence of large (>5 cm(2)) articular cartilage defects involving underlying bone, current tissue-engineered therapies only address small defects. Tissue-engineered, anatomically shaped, native-like implants may address the need for off-the-shelf, tissue-repairing therapies for large cartilage lesions. This study fabricated an osteochondral construct of translationally relevant geometry with robust functional properties. Scaffold-free, self-assembled neocartilage served as the chondral phase, and porous hydroxyapatite served as the osseous phase of the osteochondral constructs. Constructs in the shape and size of an ovine femoral condyle (31 × 14 mm) were assembled at day 4 (early) or day 10 (late) of neocartilage maturation. Early osteochondral assembly increased the interfacial interdigitation depth by 244%, interdigitation frequency by 438%, interfacial shear modulus by 243-fold, and ultimate interfacial shear strength by 4.9-fold, compared to late assembly. Toward the development of a bioprosthesis for the repair of cartilage lesions encompassing up to an entire condylar surface, this study generated a large, anatomically shaped osteochondral construct with robust interfacial mechanical properties and native-like neocartilage interdigitation. Nature Publishing Group UK 2021-08-06 /pmc/articles/PMC8346478/ /pubmed/34362933 http://dx.doi.org/10.1038/s41536-021-00152-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Brown, Wendy E.
Huang, Brian J.
Hu, Jerry C.
Athanasiou, Kyriacos A.
Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties
title Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties
title_full Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties
title_fullStr Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties
title_full_unstemmed Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties
title_short Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties
title_sort engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346478/
https://www.ncbi.nlm.nih.gov/pubmed/34362933
http://dx.doi.org/10.1038/s41536-021-00152-0
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