Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay

Metastasis is the main cause of cancer-associated morbidity which will account for ∼ 600,000 deaths in the USA in 2021. Defining new mechanisms that drive cancer metastasis is vital for developing new therapeutic strategies and improving clinical outcomes for cancer patients. Herein, we describe a r...

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Detalles Bibliográficos
Autores principales: Aslan, Merve, Hsu, En-Chi, Liu, Shiqin, Stoyanova, Tanya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Protocols
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346651/
https://www.ncbi.nlm.nih.gov/pubmed/34377838
http://dx.doi.org/10.1093/biomethods/bpab014
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author Aslan, Merve
Hsu, En-Chi
Liu, Shiqin
Stoyanova, Tanya
author_facet Aslan, Merve
Hsu, En-Chi
Liu, Shiqin
Stoyanova, Tanya
author_sort Aslan, Merve
collection PubMed
description Metastasis is the main cause of cancer-associated morbidity which will account for ∼ 600,000 deaths in the USA in 2021. Defining new mechanisms that drive cancer metastasis is vital for developing new therapeutic strategies and improving clinical outcomes for cancer patients. Herein, we describe a recently established 3D Matrigel drop invasion assay to measure cancer cell invasion and migration capability in vitro. This assay is a versatile and simple tool to test the ability of cells to invade and migrate, test the functional role of genes of interest in cell invasion and migration, analyze the localization of the target proteins at the cell invasion edge in situ, and screen drug effects on cancer cell invasion and migration.
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spelling pubmed-83466512021-08-09 Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay Aslan, Merve Hsu, En-Chi Liu, Shiqin Stoyanova, Tanya Biol Methods Protoc Protocols Metastasis is the main cause of cancer-associated morbidity which will account for ∼ 600,000 deaths in the USA in 2021. Defining new mechanisms that drive cancer metastasis is vital for developing new therapeutic strategies and improving clinical outcomes for cancer patients. Herein, we describe a recently established 3D Matrigel drop invasion assay to measure cancer cell invasion and migration capability in vitro. This assay is a versatile and simple tool to test the ability of cells to invade and migrate, test the functional role of genes of interest in cell invasion and migration, analyze the localization of the target proteins at the cell invasion edge in situ, and screen drug effects on cancer cell invasion and migration. Oxford University Press 2021-07-21 /pmc/articles/PMC8346651/ /pubmed/34377838 http://dx.doi.org/10.1093/biomethods/bpab014 Text en © The Author(s) 2021. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Protocols
Aslan, Merve
Hsu, En-Chi
Liu, Shiqin
Stoyanova, Tanya
Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay
title Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay
title_full Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay
title_fullStr Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay
title_full_unstemmed Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay
title_short Quantifying the invasion and migration ability of cancer cells with a 3D Matrigel drop invasion assay
title_sort quantifying the invasion and migration ability of cancer cells with a 3d matrigel drop invasion assay
topic Protocols
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346651/
https://www.ncbi.nlm.nih.gov/pubmed/34377838
http://dx.doi.org/10.1093/biomethods/bpab014
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