Cargando…

Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov.

Sampling campaigns in Greenland and Svalbard were executed to explore fungal diversity in cold habitats. Three very abundant groups of strains were discovered, consisting either of recently described or of yet-undescribed psychrophilic and oligotrophic yeasts and dimorphic fungi, accounting for arou...

Descripción completa

Detalles Bibliográficos
Autores principales: Perini, Laura, Andrejašič, Kristina, Gostinčar, Cene, Gunde-Cimerman, Nina, Zalar, Polona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346769/
https://www.ncbi.nlm.nih.gov/pubmed/33502296
http://dx.doi.org/10.1099/ijsem.0.004655
_version_ 1783734946438840320
author Perini, Laura
Andrejašič, Kristina
Gostinčar, Cene
Gunde-Cimerman, Nina
Zalar, Polona
author_facet Perini, Laura
Andrejašič, Kristina
Gostinčar, Cene
Gunde-Cimerman, Nina
Zalar, Polona
author_sort Perini, Laura
collection PubMed
description Sampling campaigns in Greenland and Svalbard were executed to explore fungal diversity in cold habitats. Three very abundant groups of strains were discovered, consisting either of recently described or of yet-undescribed psychrophilic and oligotrophic yeasts and dimorphic fungi, accounting for around 50 % of the total cultivable diversity of basidiomycetes in our studies. The occurrence of these taxa has also been demonstrated by culture-independent methods. Based on phylogenetic analyses of ribosomal gene cluster sequences (D1/D2 domains of 28S (LSU), 18S (SSU), ITS with 5.8S rDNA) and sequences of protein-coding genes for elongation factor one alpha (TEF), cytochrome b (CYTB) and two subunits of the RNA polymerase II (RPB1 and RPB2) obtained from pure cultures, the isolated taxa presented in this study belong to Basidiomycota, subphylum Pucciniomycotina, class Microbotryomycetes, family Camptobasidiaceae. The dataset of the sequences supported the recognition of three species: Camptobasidium gelus, Camptobasidium arcticum sp. nov. (ex-type strain EXF-12713) and Psychromyces glacialis gen. and sp. nov. (ex-type strain EXF-13111). Camptobasidium gelus was found in the Svalbard and Greenland samples, while representatives of the here proposed new species, C. arcticum, were found only in the Greenland Ice Sheet. Psychromyces gen. nov. was erected for the dimorphic/filamentous isolates found in Svalbard and Greenland glacial environments. The taxon, for which the invalid name ‘Rhodotorula svalbardensis’ has been used, belongs to this genus. Based on ribosomal genes, Camptobasidium arcticum and Psychromyces glacialis are related, phylogenetically most closely related to the genera Glaciozyma and Cryolevonia. Seven genes phylogeny restricted to taxa with available sequences, supported the placement of Psychromyces to Camptobasidiaceae.
format Online
Article
Text
id pubmed-8346769
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-83467692021-08-09 Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov. Perini, Laura Andrejašič, Kristina Gostinčar, Cene Gunde-Cimerman, Nina Zalar, Polona Int J Syst Evol Microbiol Taxonomic Description Sampling campaigns in Greenland and Svalbard were executed to explore fungal diversity in cold habitats. Three very abundant groups of strains were discovered, consisting either of recently described or of yet-undescribed psychrophilic and oligotrophic yeasts and dimorphic fungi, accounting for around 50 % of the total cultivable diversity of basidiomycetes in our studies. The occurrence of these taxa has also been demonstrated by culture-independent methods. Based on phylogenetic analyses of ribosomal gene cluster sequences (D1/D2 domains of 28S (LSU), 18S (SSU), ITS with 5.8S rDNA) and sequences of protein-coding genes for elongation factor one alpha (TEF), cytochrome b (CYTB) and two subunits of the RNA polymerase II (RPB1 and RPB2) obtained from pure cultures, the isolated taxa presented in this study belong to Basidiomycota, subphylum Pucciniomycotina, class Microbotryomycetes, family Camptobasidiaceae. The dataset of the sequences supported the recognition of three species: Camptobasidium gelus, Camptobasidium arcticum sp. nov. (ex-type strain EXF-12713) and Psychromyces glacialis gen. and sp. nov. (ex-type strain EXF-13111). Camptobasidium gelus was found in the Svalbard and Greenland samples, while representatives of the here proposed new species, C. arcticum, were found only in the Greenland Ice Sheet. Psychromyces gen. nov. was erected for the dimorphic/filamentous isolates found in Svalbard and Greenland glacial environments. The taxon, for which the invalid name ‘Rhodotorula svalbardensis’ has been used, belongs to this genus. Based on ribosomal genes, Camptobasidium arcticum and Psychromyces glacialis are related, phylogenetically most closely related to the genera Glaciozyma and Cryolevonia. Seven genes phylogeny restricted to taxa with available sequences, supported the placement of Psychromyces to Camptobasidiaceae. Microbiology Society 2021-01-27 /pmc/articles/PMC8346769/ /pubmed/33502296 http://dx.doi.org/10.1099/ijsem.0.004655 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Taxonomic Description
Perini, Laura
Andrejašič, Kristina
Gostinčar, Cene
Gunde-Cimerman, Nina
Zalar, Polona
Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov.
title Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov.
title_full Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov.
title_fullStr Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov.
title_full_unstemmed Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov.
title_short Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov.
title_sort greenland and svalbard glaciers host unknown basidiomycetes: the yeast camptobasidium arcticum sp. nov. and the dimorphic psychromyces glacialis gen. and sp. nov.
topic Taxonomic Description
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346769/
https://www.ncbi.nlm.nih.gov/pubmed/33502296
http://dx.doi.org/10.1099/ijsem.0.004655
work_keys_str_mv AT perinilaura greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov
AT andrejasickristina greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov
AT gostincarcene greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov
AT gundecimermannina greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov
AT zalarpolona greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov