Cargando…
Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov.
Sampling campaigns in Greenland and Svalbard were executed to explore fungal diversity in cold habitats. Three very abundant groups of strains were discovered, consisting either of recently described or of yet-undescribed psychrophilic and oligotrophic yeasts and dimorphic fungi, accounting for arou...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346769/ https://www.ncbi.nlm.nih.gov/pubmed/33502296 http://dx.doi.org/10.1099/ijsem.0.004655 |
_version_ | 1783734946438840320 |
---|---|
author | Perini, Laura Andrejašič, Kristina Gostinčar, Cene Gunde-Cimerman, Nina Zalar, Polona |
author_facet | Perini, Laura Andrejašič, Kristina Gostinčar, Cene Gunde-Cimerman, Nina Zalar, Polona |
author_sort | Perini, Laura |
collection | PubMed |
description | Sampling campaigns in Greenland and Svalbard were executed to explore fungal diversity in cold habitats. Three very abundant groups of strains were discovered, consisting either of recently described or of yet-undescribed psychrophilic and oligotrophic yeasts and dimorphic fungi, accounting for around 50 % of the total cultivable diversity of basidiomycetes in our studies. The occurrence of these taxa has also been demonstrated by culture-independent methods. Based on phylogenetic analyses of ribosomal gene cluster sequences (D1/D2 domains of 28S (LSU), 18S (SSU), ITS with 5.8S rDNA) and sequences of protein-coding genes for elongation factor one alpha (TEF), cytochrome b (CYTB) and two subunits of the RNA polymerase II (RPB1 and RPB2) obtained from pure cultures, the isolated taxa presented in this study belong to Basidiomycota, subphylum Pucciniomycotina, class Microbotryomycetes, family Camptobasidiaceae. The dataset of the sequences supported the recognition of three species: Camptobasidium gelus, Camptobasidium arcticum sp. nov. (ex-type strain EXF-12713) and Psychromyces glacialis gen. and sp. nov. (ex-type strain EXF-13111). Camptobasidium gelus was found in the Svalbard and Greenland samples, while representatives of the here proposed new species, C. arcticum, were found only in the Greenland Ice Sheet. Psychromyces gen. nov. was erected for the dimorphic/filamentous isolates found in Svalbard and Greenland glacial environments. The taxon, for which the invalid name ‘Rhodotorula svalbardensis’ has been used, belongs to this genus. Based on ribosomal genes, Camptobasidium arcticum and Psychromyces glacialis are related, phylogenetically most closely related to the genera Glaciozyma and Cryolevonia. Seven genes phylogeny restricted to taxa with available sequences, supported the placement of Psychromyces to Camptobasidiaceae. |
format | Online Article Text |
id | pubmed-8346769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-83467692021-08-09 Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov. Perini, Laura Andrejašič, Kristina Gostinčar, Cene Gunde-Cimerman, Nina Zalar, Polona Int J Syst Evol Microbiol Taxonomic Description Sampling campaigns in Greenland and Svalbard were executed to explore fungal diversity in cold habitats. Three very abundant groups of strains were discovered, consisting either of recently described or of yet-undescribed psychrophilic and oligotrophic yeasts and dimorphic fungi, accounting for around 50 % of the total cultivable diversity of basidiomycetes in our studies. The occurrence of these taxa has also been demonstrated by culture-independent methods. Based on phylogenetic analyses of ribosomal gene cluster sequences (D1/D2 domains of 28S (LSU), 18S (SSU), ITS with 5.8S rDNA) and sequences of protein-coding genes for elongation factor one alpha (TEF), cytochrome b (CYTB) and two subunits of the RNA polymerase II (RPB1 and RPB2) obtained from pure cultures, the isolated taxa presented in this study belong to Basidiomycota, subphylum Pucciniomycotina, class Microbotryomycetes, family Camptobasidiaceae. The dataset of the sequences supported the recognition of three species: Camptobasidium gelus, Camptobasidium arcticum sp. nov. (ex-type strain EXF-12713) and Psychromyces glacialis gen. and sp. nov. (ex-type strain EXF-13111). Camptobasidium gelus was found in the Svalbard and Greenland samples, while representatives of the here proposed new species, C. arcticum, were found only in the Greenland Ice Sheet. Psychromyces gen. nov. was erected for the dimorphic/filamentous isolates found in Svalbard and Greenland glacial environments. The taxon, for which the invalid name ‘Rhodotorula svalbardensis’ has been used, belongs to this genus. Based on ribosomal genes, Camptobasidium arcticum and Psychromyces glacialis are related, phylogenetically most closely related to the genera Glaciozyma and Cryolevonia. Seven genes phylogeny restricted to taxa with available sequences, supported the placement of Psychromyces to Camptobasidiaceae. Microbiology Society 2021-01-27 /pmc/articles/PMC8346769/ /pubmed/33502296 http://dx.doi.org/10.1099/ijsem.0.004655 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
spellingShingle | Taxonomic Description Perini, Laura Andrejašič, Kristina Gostinčar, Cene Gunde-Cimerman, Nina Zalar, Polona Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov. |
title | Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov. |
title_full | Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov. |
title_fullStr | Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov. |
title_full_unstemmed | Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov. |
title_short | Greenland and Svalbard glaciers host unknown basidiomycetes: the yeast Camptobasidium arcticum sp. nov. and the dimorphic Psychromyces glacialis gen. and sp. nov. |
title_sort | greenland and svalbard glaciers host unknown basidiomycetes: the yeast camptobasidium arcticum sp. nov. and the dimorphic psychromyces glacialis gen. and sp. nov. |
topic | Taxonomic Description |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346769/ https://www.ncbi.nlm.nih.gov/pubmed/33502296 http://dx.doi.org/10.1099/ijsem.0.004655 |
work_keys_str_mv | AT perinilaura greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov AT andrejasickristina greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov AT gostincarcene greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov AT gundecimermannina greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov AT zalarpolona greenlandandsvalbardglaciershostunknownbasidiomycetestheyeastcamptobasidiumarcticumspnovandthedimorphicpsychromycesglacialisgenandspnov |