Cargando…

Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo

Small diameter (<6 mm) vessel grafts still pose a challenge for scientists worldwide. Decellularised umbilical artery (dUA) remains promising as small diameter tissue engineered vascular graft (TEVG), yet their immunogenicity remains unknown. Herein, we evaluated the host immune responses, with a...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahlmann, Alexander Høgsted, Fang, Shu, Mortensen, Sussi Bagge, Andersen, Line Weis, Pedersen, Pernille Gejl, Callesen, Johanne Juel, Bak, Sara Thornby, Lambertsen, Kate Lykke, Andersen, Ditte Caroline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347020/
https://www.ncbi.nlm.nih.gov/pubmed/34360744
http://dx.doi.org/10.3390/ijms22157981
_version_ 1783734983063502848
author Ahlmann, Alexander Høgsted
Fang, Shu
Mortensen, Sussi Bagge
Andersen, Line Weis
Pedersen, Pernille Gejl
Callesen, Johanne Juel
Bak, Sara Thornby
Lambertsen, Kate Lykke
Andersen, Ditte Caroline
author_facet Ahlmann, Alexander Høgsted
Fang, Shu
Mortensen, Sussi Bagge
Andersen, Line Weis
Pedersen, Pernille Gejl
Callesen, Johanne Juel
Bak, Sara Thornby
Lambertsen, Kate Lykke
Andersen, Ditte Caroline
author_sort Ahlmann, Alexander Høgsted
collection PubMed
description Small diameter (<6 mm) vessel grafts still pose a challenge for scientists worldwide. Decellularised umbilical artery (dUA) remains promising as small diameter tissue engineered vascular graft (TEVG), yet their immunogenicity remains unknown. Herein, we evaluated the host immune responses, with a focus on the innate part, towards human dUA implantation in mice, and confirmed our findings in an ex vivo allogeneic human setup. Overall, we did not observe any differences in the number of circulating white blood cells nor the number of monocytes among three groups of mice (1) dUA patch; (2) Sham; and (3) Mock throughout the study (day −7 to 28). Likewise, we found no difference in systemic inflammatory and anti-inflammatory cytokine levels between groups. However, a massive local remodelling response with M2 macrophages were observed in the dUA at day 28, whereas M1 macrophages were less frequent. Moreover, human monocytes from allogeneic individuals were differentiated into macrophages and exposed to lyophilised dUA to maximize an eventual M1 response. Yet, dUA did not elicit any immediate M1 response as determined by the absence of CCR7 and CXCL10. Together this suggests that human dUA elicits a minimal pro-inflammatory response further supporting its use as a TEVG in an allogeneic setup.
format Online
Article
Text
id pubmed-8347020
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83470202021-08-08 Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo Ahlmann, Alexander Høgsted Fang, Shu Mortensen, Sussi Bagge Andersen, Line Weis Pedersen, Pernille Gejl Callesen, Johanne Juel Bak, Sara Thornby Lambertsen, Kate Lykke Andersen, Ditte Caroline Int J Mol Sci Article Small diameter (<6 mm) vessel grafts still pose a challenge for scientists worldwide. Decellularised umbilical artery (dUA) remains promising as small diameter tissue engineered vascular graft (TEVG), yet their immunogenicity remains unknown. Herein, we evaluated the host immune responses, with a focus on the innate part, towards human dUA implantation in mice, and confirmed our findings in an ex vivo allogeneic human setup. Overall, we did not observe any differences in the number of circulating white blood cells nor the number of monocytes among three groups of mice (1) dUA patch; (2) Sham; and (3) Mock throughout the study (day −7 to 28). Likewise, we found no difference in systemic inflammatory and anti-inflammatory cytokine levels between groups. However, a massive local remodelling response with M2 macrophages were observed in the dUA at day 28, whereas M1 macrophages were less frequent. Moreover, human monocytes from allogeneic individuals were differentiated into macrophages and exposed to lyophilised dUA to maximize an eventual M1 response. Yet, dUA did not elicit any immediate M1 response as determined by the absence of CCR7 and CXCL10. Together this suggests that human dUA elicits a minimal pro-inflammatory response further supporting its use as a TEVG in an allogeneic setup. MDPI 2021-07-26 /pmc/articles/PMC8347020/ /pubmed/34360744 http://dx.doi.org/10.3390/ijms22157981 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahlmann, Alexander Høgsted
Fang, Shu
Mortensen, Sussi Bagge
Andersen, Line Weis
Pedersen, Pernille Gejl
Callesen, Johanne Juel
Bak, Sara Thornby
Lambertsen, Kate Lykke
Andersen, Ditte Caroline
Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo
title Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo
title_full Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo
title_fullStr Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo
title_full_unstemmed Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo
title_short Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo
title_sort decellularised human umbilical artery as a vascular graft elicits minimal pro-inflammatory host response ex vivo and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347020/
https://www.ncbi.nlm.nih.gov/pubmed/34360744
http://dx.doi.org/10.3390/ijms22157981
work_keys_str_mv AT ahlmannalexanderhøgsted decellularisedhumanumbilicalarteryasavasculargraftelicitsminimalproinflammatoryhostresponseexvivoandinvivo
AT fangshu decellularisedhumanumbilicalarteryasavasculargraftelicitsminimalproinflammatoryhostresponseexvivoandinvivo
AT mortensensussibagge decellularisedhumanumbilicalarteryasavasculargraftelicitsminimalproinflammatoryhostresponseexvivoandinvivo
AT andersenlineweis decellularisedhumanumbilicalarteryasavasculargraftelicitsminimalproinflammatoryhostresponseexvivoandinvivo
AT pedersenpernillegejl decellularisedhumanumbilicalarteryasavasculargraftelicitsminimalproinflammatoryhostresponseexvivoandinvivo
AT callesenjohannejuel decellularisedhumanumbilicalarteryasavasculargraftelicitsminimalproinflammatoryhostresponseexvivoandinvivo
AT baksarathornby decellularisedhumanumbilicalarteryasavasculargraftelicitsminimalproinflammatoryhostresponseexvivoandinvivo
AT lambertsenkatelykke decellularisedhumanumbilicalarteryasavasculargraftelicitsminimalproinflammatoryhostresponseexvivoandinvivo
AT andersendittecaroline decellularisedhumanumbilicalarteryasavasculargraftelicitsminimalproinflammatoryhostresponseexvivoandinvivo