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Benefits of Innovative and Fully Water-Compatible Stationary Phases of Thin-Film Microextraction (TFME) Blades

Octadecyl (C(18)) groups are arguably the most popular ligands used for preparation of solid phase microextraction (SPME) devices. However, conventional C(18)-bonded silica particles are not fully compatible with the nearly 100% aqueous composition of typical biological samples (e.g., plasma, saliva...

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Autores principales: Sobczak, Łukasz, Kołodziej, Dominika, Goryński, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347298/
https://www.ncbi.nlm.nih.gov/pubmed/34361565
http://dx.doi.org/10.3390/molecules26154413
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author Sobczak, Łukasz
Kołodziej, Dominika
Goryński, Krzysztof
author_facet Sobczak, Łukasz
Kołodziej, Dominika
Goryński, Krzysztof
author_sort Sobczak, Łukasz
collection PubMed
description Octadecyl (C(18)) groups are arguably the most popular ligands used for preparation of solid phase microextraction (SPME) devices. However, conventional C(18)-bonded silica particles are not fully compatible with the nearly 100% aqueous composition of typical biological samples (e.g., plasma, saliva, or urine). This study presents the first evaluation of thin-film SPME devices coated with special water-compatible C(18)-bonded particles. Device performance was assessed by extracting a mixture of 30 model compounds that exhibited various chemical structures and properties, such as hydrophobicity. Additionally, nine unique compositions of desorption solvents were tested. Thin-film SPME devices coated with C(18)-bonded silica particles with polar end-capping groups (10 µm) were compared with conventional trimethylsilane end-capped C(18)-bonded silica particles of various sizes (5, 10, and 45 µm) and characteristics. Polar end-capped particles provided the best extraction efficacy and were characterized by the strongest correlations between the efficacy of the extraction process and the hydrophobicity of the analytes. The results suggest that the original features of octadecyl ligands are best preserved in aqueous conditions by polar end-capped particles, unlike with conventional trimethylsilane end-capped particles that are currently used to prepare SPME devices. The benefits associated with this improved type of coating encourage further implementation of microextractraction as greener alternative to the traditional sample preparation methods.
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spelling pubmed-83472982021-08-08 Benefits of Innovative and Fully Water-Compatible Stationary Phases of Thin-Film Microextraction (TFME) Blades Sobczak, Łukasz Kołodziej, Dominika Goryński, Krzysztof Molecules Article Octadecyl (C(18)) groups are arguably the most popular ligands used for preparation of solid phase microextraction (SPME) devices. However, conventional C(18)-bonded silica particles are not fully compatible with the nearly 100% aqueous composition of typical biological samples (e.g., plasma, saliva, or urine). This study presents the first evaluation of thin-film SPME devices coated with special water-compatible C(18)-bonded particles. Device performance was assessed by extracting a mixture of 30 model compounds that exhibited various chemical structures and properties, such as hydrophobicity. Additionally, nine unique compositions of desorption solvents were tested. Thin-film SPME devices coated with C(18)-bonded silica particles with polar end-capping groups (10 µm) were compared with conventional trimethylsilane end-capped C(18)-bonded silica particles of various sizes (5, 10, and 45 µm) and characteristics. Polar end-capped particles provided the best extraction efficacy and were characterized by the strongest correlations between the efficacy of the extraction process and the hydrophobicity of the analytes. The results suggest that the original features of octadecyl ligands are best preserved in aqueous conditions by polar end-capped particles, unlike with conventional trimethylsilane end-capped particles that are currently used to prepare SPME devices. The benefits associated with this improved type of coating encourage further implementation of microextractraction as greener alternative to the traditional sample preparation methods. MDPI 2021-07-21 /pmc/articles/PMC8347298/ /pubmed/34361565 http://dx.doi.org/10.3390/molecules26154413 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sobczak, Łukasz
Kołodziej, Dominika
Goryński, Krzysztof
Benefits of Innovative and Fully Water-Compatible Stationary Phases of Thin-Film Microextraction (TFME) Blades
title Benefits of Innovative and Fully Water-Compatible Stationary Phases of Thin-Film Microextraction (TFME) Blades
title_full Benefits of Innovative and Fully Water-Compatible Stationary Phases of Thin-Film Microextraction (TFME) Blades
title_fullStr Benefits of Innovative and Fully Water-Compatible Stationary Phases of Thin-Film Microextraction (TFME) Blades
title_full_unstemmed Benefits of Innovative and Fully Water-Compatible Stationary Phases of Thin-Film Microextraction (TFME) Blades
title_short Benefits of Innovative and Fully Water-Compatible Stationary Phases of Thin-Film Microextraction (TFME) Blades
title_sort benefits of innovative and fully water-compatible stationary phases of thin-film microextraction (tfme) blades
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347298/
https://www.ncbi.nlm.nih.gov/pubmed/34361565
http://dx.doi.org/10.3390/molecules26154413
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