Cargando…

Aloysia Citrodora Essential Oil Inhibits Melanoma Cell Growth and Migration by Targeting HB-EGF-EGFR Signaling

Patients diagnosed with melanoma have a poor prognosis due to regional invasion and metastases. The receptor tyrosine kinase epidermal growth factor receptor (EGFR) is found in a subtype of melanoma with a poor prognosis and contributes to drug resistance. Aloysia citrodora essential oil (ALOC-EO) p...

Descripción completa

Detalles Bibliográficos
Autores principales: Salama, Yousef, Jaradat, Nidal, Hattori, Koichi, Heissig, Beate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347434/
https://www.ncbi.nlm.nih.gov/pubmed/34360915
http://dx.doi.org/10.3390/ijms22158151
_version_ 1783735088691806208
author Salama, Yousef
Jaradat, Nidal
Hattori, Koichi
Heissig, Beate
author_facet Salama, Yousef
Jaradat, Nidal
Hattori, Koichi
Heissig, Beate
author_sort Salama, Yousef
collection PubMed
description Patients diagnosed with melanoma have a poor prognosis due to regional invasion and metastases. The receptor tyrosine kinase epidermal growth factor receptor (EGFR) is found in a subtype of melanoma with a poor prognosis and contributes to drug resistance. Aloysia citrodora essential oil (ALOC-EO) possesses an antitumor effect. Understanding signaling pathways that contribute to the antitumor of ALOC-EO is important to identify novel tumor types that can be targeted by ALOC-EO. Here, we investigated the effects of ALOC-EO on melanoma growth and tumor cell migration. ALOC-EO blocked melanoma growth in vitro and impaired primary tumor cell growth in vivo. Mechanistically, ALOC-EO blocked heparin-binding-epidermal growth factor (HB-EGF)-induced EGFR signaling and suppressed ERK1/2 phosphorylation. Myelosuppressive drugs upregulated HB-EGF and EGFR expression in melanoma cells. Cotreatment of myelosuppressive drugs with ALOC-EO improved the antitumor activity and inhibited the expression of matrix metalloproteinase-7 and -9 and a disintegrin and metalloproteinase domain-containing protein9. In summary, our study demonstrates that ALOC-EO blocks EGFR and ERK1/2 signaling, with preclinical efficacy as a monotherapy or in combination with myelosuppressive drugs in melanoma.
format Online
Article
Text
id pubmed-8347434
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83474342021-08-08 Aloysia Citrodora Essential Oil Inhibits Melanoma Cell Growth and Migration by Targeting HB-EGF-EGFR Signaling Salama, Yousef Jaradat, Nidal Hattori, Koichi Heissig, Beate Int J Mol Sci Article Patients diagnosed with melanoma have a poor prognosis due to regional invasion and metastases. The receptor tyrosine kinase epidermal growth factor receptor (EGFR) is found in a subtype of melanoma with a poor prognosis and contributes to drug resistance. Aloysia citrodora essential oil (ALOC-EO) possesses an antitumor effect. Understanding signaling pathways that contribute to the antitumor of ALOC-EO is important to identify novel tumor types that can be targeted by ALOC-EO. Here, we investigated the effects of ALOC-EO on melanoma growth and tumor cell migration. ALOC-EO blocked melanoma growth in vitro and impaired primary tumor cell growth in vivo. Mechanistically, ALOC-EO blocked heparin-binding-epidermal growth factor (HB-EGF)-induced EGFR signaling and suppressed ERK1/2 phosphorylation. Myelosuppressive drugs upregulated HB-EGF and EGFR expression in melanoma cells. Cotreatment of myelosuppressive drugs with ALOC-EO improved the antitumor activity and inhibited the expression of matrix metalloproteinase-7 and -9 and a disintegrin and metalloproteinase domain-containing protein9. In summary, our study demonstrates that ALOC-EO blocks EGFR and ERK1/2 signaling, with preclinical efficacy as a monotherapy or in combination with myelosuppressive drugs in melanoma. MDPI 2021-07-29 /pmc/articles/PMC8347434/ /pubmed/34360915 http://dx.doi.org/10.3390/ijms22158151 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salama, Yousef
Jaradat, Nidal
Hattori, Koichi
Heissig, Beate
Aloysia Citrodora Essential Oil Inhibits Melanoma Cell Growth and Migration by Targeting HB-EGF-EGFR Signaling
title Aloysia Citrodora Essential Oil Inhibits Melanoma Cell Growth and Migration by Targeting HB-EGF-EGFR Signaling
title_full Aloysia Citrodora Essential Oil Inhibits Melanoma Cell Growth and Migration by Targeting HB-EGF-EGFR Signaling
title_fullStr Aloysia Citrodora Essential Oil Inhibits Melanoma Cell Growth and Migration by Targeting HB-EGF-EGFR Signaling
title_full_unstemmed Aloysia Citrodora Essential Oil Inhibits Melanoma Cell Growth and Migration by Targeting HB-EGF-EGFR Signaling
title_short Aloysia Citrodora Essential Oil Inhibits Melanoma Cell Growth and Migration by Targeting HB-EGF-EGFR Signaling
title_sort aloysia citrodora essential oil inhibits melanoma cell growth and migration by targeting hb-egf-egfr signaling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347434/
https://www.ncbi.nlm.nih.gov/pubmed/34360915
http://dx.doi.org/10.3390/ijms22158151
work_keys_str_mv AT salamayousef aloysiacitrodoraessentialoilinhibitsmelanomacellgrowthandmigrationbytargetinghbegfegfrsignaling
AT jaradatnidal aloysiacitrodoraessentialoilinhibitsmelanomacellgrowthandmigrationbytargetinghbegfegfrsignaling
AT hattorikoichi aloysiacitrodoraessentialoilinhibitsmelanomacellgrowthandmigrationbytargetinghbegfegfrsignaling
AT heissigbeate aloysiacitrodoraessentialoilinhibitsmelanomacellgrowthandmigrationbytargetinghbegfegfrsignaling