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The Application of an Omentum Graft or Flap in Spinal Cord Injury

Background: Spinal cord injury (SCI) causes a primary injury at the lesion site and triggers a secondary injury and prolonged inflammation. There has been no definitive treatment till now. Promoting angiogenesis is one of the most important strategies for functional recovery after SCI. The omentum,...

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Autores principales: Fay, Li-Yu, Lin, Yan-Ru, Liou, Dann-Ying, Chiu, Chuan-Wen, Yeh, Mei-Yin, Huang, Wen-Cheng, Wu, Jau-Ching, Tsai, May-Jywan, Cheng, Henrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347514/
https://www.ncbi.nlm.nih.gov/pubmed/34360697
http://dx.doi.org/10.3390/ijms22157930
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author Fay, Li-Yu
Lin, Yan-Ru
Liou, Dann-Ying
Chiu, Chuan-Wen
Yeh, Mei-Yin
Huang, Wen-Cheng
Wu, Jau-Ching
Tsai, May-Jywan
Cheng, Henrich
author_facet Fay, Li-Yu
Lin, Yan-Ru
Liou, Dann-Ying
Chiu, Chuan-Wen
Yeh, Mei-Yin
Huang, Wen-Cheng
Wu, Jau-Ching
Tsai, May-Jywan
Cheng, Henrich
author_sort Fay, Li-Yu
collection PubMed
description Background: Spinal cord injury (SCI) causes a primary injury at the lesion site and triggers a secondary injury and prolonged inflammation. There has been no definitive treatment till now. Promoting angiogenesis is one of the most important strategies for functional recovery after SCI. The omentum, abundant in blood and lymph vessels, possesses the potent ability of tissue regeneration. Methods: The present work examines the efficacy of autologous omentum, either as a flap (with vascular connection intact) or graft (severed vascular connection), on spinal nerve regeneration. After contusive SCI in rats, a thin sheath of omentum was grafted to the injured spinal cord. Results: Omental graft improved behavior scores significantly from the 3rd to 6th week after injury (6th week, 5.5 ± 0.5 vs. 8.6 ± 1.3, p < 0.05). Furthermore, the reduction in cavity and the preservation of class III β-tubulin-positive nerve fibers in the injury area was noted. Next, the free omental flap was transposed to a completely transected SCI in rats through a pre-implanted tunnel. The flap remained vascularized and survived well several weeks after the operation. At 16 weeks post-treatment, SCI rats with omentum flap treatment displayed the preservation of significantly more nerve fibers (p < 0.05) and a reduced injured cavity, though locomotor scores were similar. Conclusions: Taken together, the findings of this study indicate that treatment with an omental graft or transposition of an omental flap on an injured spinal cord has a positive effect on nerve protection and tissue preservation in SCI rats. The current data highlight the importance of omentum in clinical applications.
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spelling pubmed-83475142021-08-08 The Application of an Omentum Graft or Flap in Spinal Cord Injury Fay, Li-Yu Lin, Yan-Ru Liou, Dann-Ying Chiu, Chuan-Wen Yeh, Mei-Yin Huang, Wen-Cheng Wu, Jau-Ching Tsai, May-Jywan Cheng, Henrich Int J Mol Sci Article Background: Spinal cord injury (SCI) causes a primary injury at the lesion site and triggers a secondary injury and prolonged inflammation. There has been no definitive treatment till now. Promoting angiogenesis is one of the most important strategies for functional recovery after SCI. The omentum, abundant in blood and lymph vessels, possesses the potent ability of tissue regeneration. Methods: The present work examines the efficacy of autologous omentum, either as a flap (with vascular connection intact) or graft (severed vascular connection), on spinal nerve regeneration. After contusive SCI in rats, a thin sheath of omentum was grafted to the injured spinal cord. Results: Omental graft improved behavior scores significantly from the 3rd to 6th week after injury (6th week, 5.5 ± 0.5 vs. 8.6 ± 1.3, p < 0.05). Furthermore, the reduction in cavity and the preservation of class III β-tubulin-positive nerve fibers in the injury area was noted. Next, the free omental flap was transposed to a completely transected SCI in rats through a pre-implanted tunnel. The flap remained vascularized and survived well several weeks after the operation. At 16 weeks post-treatment, SCI rats with omentum flap treatment displayed the preservation of significantly more nerve fibers (p < 0.05) and a reduced injured cavity, though locomotor scores were similar. Conclusions: Taken together, the findings of this study indicate that treatment with an omental graft or transposition of an omental flap on an injured spinal cord has a positive effect on nerve protection and tissue preservation in SCI rats. The current data highlight the importance of omentum in clinical applications. MDPI 2021-07-25 /pmc/articles/PMC8347514/ /pubmed/34360697 http://dx.doi.org/10.3390/ijms22157930 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fay, Li-Yu
Lin, Yan-Ru
Liou, Dann-Ying
Chiu, Chuan-Wen
Yeh, Mei-Yin
Huang, Wen-Cheng
Wu, Jau-Ching
Tsai, May-Jywan
Cheng, Henrich
The Application of an Omentum Graft or Flap in Spinal Cord Injury
title The Application of an Omentum Graft or Flap in Spinal Cord Injury
title_full The Application of an Omentum Graft or Flap in Spinal Cord Injury
title_fullStr The Application of an Omentum Graft or Flap in Spinal Cord Injury
title_full_unstemmed The Application of an Omentum Graft or Flap in Spinal Cord Injury
title_short The Application of an Omentum Graft or Flap in Spinal Cord Injury
title_sort application of an omentum graft or flap in spinal cord injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347514/
https://www.ncbi.nlm.nih.gov/pubmed/34360697
http://dx.doi.org/10.3390/ijms22157930
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