Cargando…
Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum
Data on the quantification of the potentially neurotrophic adipo-myokine METRNL (Meteorin-like protein) in human cerebrospinal fluid (CSF) are lacking and migration of this secreted protein across the blood–brain barrier (BBB) is uncertain. In the present pilot study, METRNL concentrations were quan...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347672/ https://www.ncbi.nlm.nih.gov/pubmed/34362056 http://dx.doi.org/10.3390/jcm10153271 |
_version_ | 1783735149362413568 |
---|---|
author | Berghoff, Martin Höpfinger, Alexandra Rajendran, Ranjithkumar Karrasch, Thomas Schmid, Andreas Schäffler, Andreas |
author_facet | Berghoff, Martin Höpfinger, Alexandra Rajendran, Ranjithkumar Karrasch, Thomas Schmid, Andreas Schäffler, Andreas |
author_sort | Berghoff, Martin |
collection | PubMed |
description | Data on the quantification of the potentially neurotrophic adipo-myokine METRNL (Meteorin-like protein) in human cerebrospinal fluid (CSF) are lacking and migration of this secreted protein across the blood–brain barrier (BBB) is uncertain. In the present pilot study, METRNL concentrations were quantified by ELISA in paired serum and CSF samples of 260 patients (107 males, 153 females) undergoing neurological evaluation. METRNL was abundant in serum (801.2 ± 378.3 pg/mL) and CSF (1007.2 ± 624.2 pg/mL) with a CSF/serum ratio of 1.4 ± 0.8. Serum METRNL levels were significantly correlated (rho = +0.521) to those in CSF. CSF METRNL concentrations were significantly correlated (rho = +0.480) with albumin CSF/serum ratios. The CSF/serum ratios of METRNL and albumin were positively correlated in Reibergram analysis (rho = 0.498), indicating that raising CSF concentrations of METRNL are mediated by increasing BBB dysfunction. The CSF concentrations of METRNL strongly increased in a stepwise manner along with increasing BBB dysfunction from grade 0 to grade 3 and with rising CSF cell count. CSF/serum ratio of METRNL also increased from grade 0 (1.2 ± 0.7) to grade 3 (3.0 ± 0.2). Furthermore, CSF levels were positively correlated with age. In conclusion, METRNL is a secreted and neurotrophic myokine that crosses over the BBB. CSF concentrations of METRNL increase with BBB dysfunction. |
format | Online Article Text |
id | pubmed-8347672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83476722021-08-08 Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum Berghoff, Martin Höpfinger, Alexandra Rajendran, Ranjithkumar Karrasch, Thomas Schmid, Andreas Schäffler, Andreas J Clin Med Article Data on the quantification of the potentially neurotrophic adipo-myokine METRNL (Meteorin-like protein) in human cerebrospinal fluid (CSF) are lacking and migration of this secreted protein across the blood–brain barrier (BBB) is uncertain. In the present pilot study, METRNL concentrations were quantified by ELISA in paired serum and CSF samples of 260 patients (107 males, 153 females) undergoing neurological evaluation. METRNL was abundant in serum (801.2 ± 378.3 pg/mL) and CSF (1007.2 ± 624.2 pg/mL) with a CSF/serum ratio of 1.4 ± 0.8. Serum METRNL levels were significantly correlated (rho = +0.521) to those in CSF. CSF METRNL concentrations were significantly correlated (rho = +0.480) with albumin CSF/serum ratios. The CSF/serum ratios of METRNL and albumin were positively correlated in Reibergram analysis (rho = 0.498), indicating that raising CSF concentrations of METRNL are mediated by increasing BBB dysfunction. The CSF concentrations of METRNL strongly increased in a stepwise manner along with increasing BBB dysfunction from grade 0 to grade 3 and with rising CSF cell count. CSF/serum ratio of METRNL also increased from grade 0 (1.2 ± 0.7) to grade 3 (3.0 ± 0.2). Furthermore, CSF levels were positively correlated with age. In conclusion, METRNL is a secreted and neurotrophic myokine that crosses over the BBB. CSF concentrations of METRNL increase with BBB dysfunction. MDPI 2021-07-24 /pmc/articles/PMC8347672/ /pubmed/34362056 http://dx.doi.org/10.3390/jcm10153271 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Berghoff, Martin Höpfinger, Alexandra Rajendran, Ranjithkumar Karrasch, Thomas Schmid, Andreas Schäffler, Andreas Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
title | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
title_full | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
title_fullStr | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
title_full_unstemmed | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
title_short | Evidence of a Muscle–Brain Axis by Quantification of the Neurotrophic Myokine METRNL (Meteorin-Like Protein) in Human Cerebrospinal Fluid and Serum |
title_sort | evidence of a muscle–brain axis by quantification of the neurotrophic myokine metrnl (meteorin-like protein) in human cerebrospinal fluid and serum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347672/ https://www.ncbi.nlm.nih.gov/pubmed/34362056 http://dx.doi.org/10.3390/jcm10153271 |
work_keys_str_mv | AT berghoffmartin evidenceofamusclebrainaxisbyquantificationoftheneurotrophicmyokinemetrnlmeteorinlikeproteininhumancerebrospinalfluidandserum AT hopfingeralexandra evidenceofamusclebrainaxisbyquantificationoftheneurotrophicmyokinemetrnlmeteorinlikeproteininhumancerebrospinalfluidandserum AT rajendranranjithkumar evidenceofamusclebrainaxisbyquantificationoftheneurotrophicmyokinemetrnlmeteorinlikeproteininhumancerebrospinalfluidandserum AT karraschthomas evidenceofamusclebrainaxisbyquantificationoftheneurotrophicmyokinemetrnlmeteorinlikeproteininhumancerebrospinalfluidandserum AT schmidandreas evidenceofamusclebrainaxisbyquantificationoftheneurotrophicmyokinemetrnlmeteorinlikeproteininhumancerebrospinalfluidandserum AT schafflerandreas evidenceofamusclebrainaxisbyquantificationoftheneurotrophicmyokinemetrnlmeteorinlikeproteininhumancerebrospinalfluidandserum |