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Staphylococcus aureus Decreases SUMOylation Host Response to Promote Intramacrophage Survival

Staphylococcus aureus is a commensal bacterium that causes severe infections in soft tissue and the bloodstream. During infection, S. aureus manipulates host cell response to facilitate its own replication and dissemination. Here, we show that S. aureus significantly decreases the level of SUMOylati...

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Autores principales: Youssouf, Nadhuma, Recasens-Zorzo, Clara, Molle, Virginie, Bossis, Guillaume, Soubeyran, Philippe, Gannoun-Zaki, Laila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347835/
https://www.ncbi.nlm.nih.gov/pubmed/34360873
http://dx.doi.org/10.3390/ijms22158108
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author Youssouf, Nadhuma
Recasens-Zorzo, Clara
Molle, Virginie
Bossis, Guillaume
Soubeyran, Philippe
Gannoun-Zaki, Laila
author_facet Youssouf, Nadhuma
Recasens-Zorzo, Clara
Molle, Virginie
Bossis, Guillaume
Soubeyran, Philippe
Gannoun-Zaki, Laila
author_sort Youssouf, Nadhuma
collection PubMed
description Staphylococcus aureus is a commensal bacterium that causes severe infections in soft tissue and the bloodstream. During infection, S. aureus manipulates host cell response to facilitate its own replication and dissemination. Here, we show that S. aureus significantly decreases the level of SUMOylation, an essential post-translational modification, in infected macrophages 24 h post-phagocytosis. The reduced level of SUMOylation correlates with a decrease in the SUMO-conjugating enzyme Ubc9. The over-expression of SUMO proteins in macrophages impaired bacterial intracellular proliferation and the inhibition of SUMOylation with ML-792 increased it. Together, these findings demonstrated for the first time the role of host SUMOylation response toward S. aureus infection.
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spelling pubmed-83478352021-08-08 Staphylococcus aureus Decreases SUMOylation Host Response to Promote Intramacrophage Survival Youssouf, Nadhuma Recasens-Zorzo, Clara Molle, Virginie Bossis, Guillaume Soubeyran, Philippe Gannoun-Zaki, Laila Int J Mol Sci Brief Report Staphylococcus aureus is a commensal bacterium that causes severe infections in soft tissue and the bloodstream. During infection, S. aureus manipulates host cell response to facilitate its own replication and dissemination. Here, we show that S. aureus significantly decreases the level of SUMOylation, an essential post-translational modification, in infected macrophages 24 h post-phagocytosis. The reduced level of SUMOylation correlates with a decrease in the SUMO-conjugating enzyme Ubc9. The over-expression of SUMO proteins in macrophages impaired bacterial intracellular proliferation and the inhibition of SUMOylation with ML-792 increased it. Together, these findings demonstrated for the first time the role of host SUMOylation response toward S. aureus infection. MDPI 2021-07-28 /pmc/articles/PMC8347835/ /pubmed/34360873 http://dx.doi.org/10.3390/ijms22158108 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Youssouf, Nadhuma
Recasens-Zorzo, Clara
Molle, Virginie
Bossis, Guillaume
Soubeyran, Philippe
Gannoun-Zaki, Laila
Staphylococcus aureus Decreases SUMOylation Host Response to Promote Intramacrophage Survival
title Staphylococcus aureus Decreases SUMOylation Host Response to Promote Intramacrophage Survival
title_full Staphylococcus aureus Decreases SUMOylation Host Response to Promote Intramacrophage Survival
title_fullStr Staphylococcus aureus Decreases SUMOylation Host Response to Promote Intramacrophage Survival
title_full_unstemmed Staphylococcus aureus Decreases SUMOylation Host Response to Promote Intramacrophage Survival
title_short Staphylococcus aureus Decreases SUMOylation Host Response to Promote Intramacrophage Survival
title_sort staphylococcus aureus decreases sumoylation host response to promote intramacrophage survival
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347835/
https://www.ncbi.nlm.nih.gov/pubmed/34360873
http://dx.doi.org/10.3390/ijms22158108
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