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Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates in Xylaria grammica KCTC 13121BP against Meloidogyne incognita

Grammicin, a polyketide metabolite produced by the endolichenic fungus Xylaria grammica KCTC 13121BP, shows strong nematicidal activity against Meloidogyne incognita. This study was performed to elucidate the grammicin biosynthesis pathway of X. grammica KCTC 13121BP and to examine the nematicidal a...

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Autores principales: Kim, Yoon Jee, Duraisamy, Kalaiselvi, Jeong, Min-Hye, Park, Sook-Young, Kim, Soonok, Lee, Yookyung, Nguyen, Van Thi, Yu, Nan Hee, Park, Ae Ran, Kim, Jin-Cheol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348278/
https://www.ncbi.nlm.nih.gov/pubmed/34361827
http://dx.doi.org/10.3390/molecules26154675
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author Kim, Yoon Jee
Duraisamy, Kalaiselvi
Jeong, Min-Hye
Park, Sook-Young
Kim, Soonok
Lee, Yookyung
Nguyen, Van Thi
Yu, Nan Hee
Park, Ae Ran
Kim, Jin-Cheol
author_facet Kim, Yoon Jee
Duraisamy, Kalaiselvi
Jeong, Min-Hye
Park, Sook-Young
Kim, Soonok
Lee, Yookyung
Nguyen, Van Thi
Yu, Nan Hee
Park, Ae Ran
Kim, Jin-Cheol
author_sort Kim, Yoon Jee
collection PubMed
description Grammicin, a polyketide metabolite produced by the endolichenic fungus Xylaria grammica KCTC 13121BP, shows strong nematicidal activity against Meloidogyne incognita. This study was performed to elucidate the grammicin biosynthesis pathway of X. grammica KCTC 13121BP and to examine the nematicidal activity of the biosynthesis intermediates and derivatives against M. incognita. Two grammicin biosynthesis intermediates were isolated from a T-DNA insertion transformant (strain TR-74) of X. grammica KCTC 13121BP and identified as 2-(hydroxymethyl)cyclohexa-2,5-diene-1,4-dione (compound 1) and 2,5-dihydroxybenzaldehyde (compound 2), which were also reported to be intermediates in the biosynthesis pathway of patulin, an isomer of grammicin. This indicates that the grammicin biosynthesis pathway overlaps almost with that of patulin, except for the last few steps. Among 13 grammicin biosynthesis intermediates and their derivatives (except grammicin), toluquinol caused the highest M. incognita J2 mortality, with an LC(50/72 h) value of 11.13 µg/mL, which is similar to grammicin with an LC(50/72 h) value of 15.95 µg/mL. In tomato pot experiments, the wettable powder type formulations (WP) of toluquinol (17.78 µg/mL) and grammicin (17.78 µg/mL) also effectively reduced gall formation on the roots of tomato plants with control values of 72.22% and 77.76%, respectively, which are much higher than abamectin (16.67%), but lower than fosthiazate (100%). The results suggest that toluquinol can be used directly as a biochemical nematicide or as a lead molecule for the development of new synthetic nematicides for the control of root-knot nematode diseases.
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spelling pubmed-83482782021-08-08 Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates in Xylaria grammica KCTC 13121BP against Meloidogyne incognita Kim, Yoon Jee Duraisamy, Kalaiselvi Jeong, Min-Hye Park, Sook-Young Kim, Soonok Lee, Yookyung Nguyen, Van Thi Yu, Nan Hee Park, Ae Ran Kim, Jin-Cheol Molecules Article Grammicin, a polyketide metabolite produced by the endolichenic fungus Xylaria grammica KCTC 13121BP, shows strong nematicidal activity against Meloidogyne incognita. This study was performed to elucidate the grammicin biosynthesis pathway of X. grammica KCTC 13121BP and to examine the nematicidal activity of the biosynthesis intermediates and derivatives against M. incognita. Two grammicin biosynthesis intermediates were isolated from a T-DNA insertion transformant (strain TR-74) of X. grammica KCTC 13121BP and identified as 2-(hydroxymethyl)cyclohexa-2,5-diene-1,4-dione (compound 1) and 2,5-dihydroxybenzaldehyde (compound 2), which were also reported to be intermediates in the biosynthesis pathway of patulin, an isomer of grammicin. This indicates that the grammicin biosynthesis pathway overlaps almost with that of patulin, except for the last few steps. Among 13 grammicin biosynthesis intermediates and their derivatives (except grammicin), toluquinol caused the highest M. incognita J2 mortality, with an LC(50/72 h) value of 11.13 µg/mL, which is similar to grammicin with an LC(50/72 h) value of 15.95 µg/mL. In tomato pot experiments, the wettable powder type formulations (WP) of toluquinol (17.78 µg/mL) and grammicin (17.78 µg/mL) also effectively reduced gall formation on the roots of tomato plants with control values of 72.22% and 77.76%, respectively, which are much higher than abamectin (16.67%), but lower than fosthiazate (100%). The results suggest that toluquinol can be used directly as a biochemical nematicide or as a lead molecule for the development of new synthetic nematicides for the control of root-knot nematode diseases. MDPI 2021-08-02 /pmc/articles/PMC8348278/ /pubmed/34361827 http://dx.doi.org/10.3390/molecules26154675 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Yoon Jee
Duraisamy, Kalaiselvi
Jeong, Min-Hye
Park, Sook-Young
Kim, Soonok
Lee, Yookyung
Nguyen, Van Thi
Yu, Nan Hee
Park, Ae Ran
Kim, Jin-Cheol
Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates in Xylaria grammica KCTC 13121BP against Meloidogyne incognita
title Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates in Xylaria grammica KCTC 13121BP against Meloidogyne incognita
title_full Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates in Xylaria grammica KCTC 13121BP against Meloidogyne incognita
title_fullStr Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates in Xylaria grammica KCTC 13121BP against Meloidogyne incognita
title_full_unstemmed Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates in Xylaria grammica KCTC 13121BP against Meloidogyne incognita
title_short Nematicidal Activity of Grammicin Biosynthesis Pathway Intermediates in Xylaria grammica KCTC 13121BP against Meloidogyne incognita
title_sort nematicidal activity of grammicin biosynthesis pathway intermediates in xylaria grammica kctc 13121bp against meloidogyne incognita
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348278/
https://www.ncbi.nlm.nih.gov/pubmed/34361827
http://dx.doi.org/10.3390/molecules26154675
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