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Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration

Peripheral nerve injuries are a common condition in which a nerve is damaged, affecting more than one million people every year. There are still no efficient therapeutic treatments for these injuries. Artificial scaffolds can offer new opportunities for nerve regeneration applications; in this frame...

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Autores principales: Scaccini, Luca, Mezzena, Roberta, De Masi, Alessia, Gagliardi, Mariacristina, Gambarotta, Giovanna, Cecchini, Marco, Tonazzini, Ilaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348329/
https://www.ncbi.nlm.nih.gov/pubmed/34360664
http://dx.doi.org/10.3390/ijms22157901
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author Scaccini, Luca
Mezzena, Roberta
De Masi, Alessia
Gagliardi, Mariacristina
Gambarotta, Giovanna
Cecchini, Marco
Tonazzini, Ilaria
author_facet Scaccini, Luca
Mezzena, Roberta
De Masi, Alessia
Gagliardi, Mariacristina
Gambarotta, Giovanna
Cecchini, Marco
Tonazzini, Ilaria
author_sort Scaccini, Luca
collection PubMed
description Peripheral nerve injuries are a common condition in which a nerve is damaged, affecting more than one million people every year. There are still no efficient therapeutic treatments for these injuries. Artificial scaffolds can offer new opportunities for nerve regeneration applications; in this framework, chitosan is emerging as a promising biomaterial. Here, we set up a simple and effective method for the production of micro-structured chitosan films by solvent casting, with high fidelity in the micro-pattern reproducibility. Three types of chitosan directional micro-grooved patterns, presenting different levels of symmetricity, were developed for application in nerve regenerative medicine: gratings (GR), isosceles triangles (ISO) and scalene triangles (SCA). The directional patterns were tested with a Schwann cell line. The most asymmetric topography (SCA), although it polarized the cell shaping less efficiently, promoted higher cell proliferation and a faster cell migration, both individually and collectively, with a higher directional persistence of motion. Overall, the use of micro-structured asymmetrical directional topographies may be exploited to enhance the nerve regeneration process mediated by chitosan scaffolds.
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spelling pubmed-83483292021-08-08 Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration Scaccini, Luca Mezzena, Roberta De Masi, Alessia Gagliardi, Mariacristina Gambarotta, Giovanna Cecchini, Marco Tonazzini, Ilaria Int J Mol Sci Article Peripheral nerve injuries are a common condition in which a nerve is damaged, affecting more than one million people every year. There are still no efficient therapeutic treatments for these injuries. Artificial scaffolds can offer new opportunities for nerve regeneration applications; in this framework, chitosan is emerging as a promising biomaterial. Here, we set up a simple and effective method for the production of micro-structured chitosan films by solvent casting, with high fidelity in the micro-pattern reproducibility. Three types of chitosan directional micro-grooved patterns, presenting different levels of symmetricity, were developed for application in nerve regenerative medicine: gratings (GR), isosceles triangles (ISO) and scalene triangles (SCA). The directional patterns were tested with a Schwann cell line. The most asymmetric topography (SCA), although it polarized the cell shaping less efficiently, promoted higher cell proliferation and a faster cell migration, both individually and collectively, with a higher directional persistence of motion. Overall, the use of micro-structured asymmetrical directional topographies may be exploited to enhance the nerve regeneration process mediated by chitosan scaffolds. MDPI 2021-07-23 /pmc/articles/PMC8348329/ /pubmed/34360664 http://dx.doi.org/10.3390/ijms22157901 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Scaccini, Luca
Mezzena, Roberta
De Masi, Alessia
Gagliardi, Mariacristina
Gambarotta, Giovanna
Cecchini, Marco
Tonazzini, Ilaria
Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration
title Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration
title_full Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration
title_fullStr Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration
title_full_unstemmed Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration
title_short Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration
title_sort chitosan micro-grooved membranes with increased asymmetry for the improvement of the schwann cell response in nerve regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348329/
https://www.ncbi.nlm.nih.gov/pubmed/34360664
http://dx.doi.org/10.3390/ijms22157901
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