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Hemostatic Patches Based on Crosslinked Chitosan Films Applied in Interventional Procedures

In this study, we manufactured biocompatible hemostatic crosslinked chitosan (CS) patches and analyzed their physicochemical and biological properties for femoral arterial puncture applications. CS is a representative hemostatic material but has some drawbacks, such as swelling, shrinkage, and britt...

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Autores principales: Lee, Moon Hyun, Lee, Dae Ryeong, Chon, Joon Woo, Chung, Dong June
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348421/
https://www.ncbi.nlm.nih.gov/pubmed/34372004
http://dx.doi.org/10.3390/polym13152402
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author Lee, Moon Hyun
Lee, Dae Ryeong
Chon, Joon Woo
Chung, Dong June
author_facet Lee, Moon Hyun
Lee, Dae Ryeong
Chon, Joon Woo
Chung, Dong June
author_sort Lee, Moon Hyun
collection PubMed
description In this study, we manufactured biocompatible hemostatic crosslinked chitosan (CS) patches and analyzed their physicochemical and biological properties for femoral arterial puncture applications. CS is a representative hemostatic material but has some drawbacks, such as swelling, shrinkage, and brittleness. Thus, it was crosslinked via a 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) coupling reaction and a nucleophilic addition reaction with citric acid (CA), glutaraldehyde (GTA), and genipin (GP) to remedy its shortcomings. The CSCA (crosslinked CS with CA/EDC), CSGTA (crosslinked CS with GTA), and CSG (crosslinked CS with GP) films showed low swelling degrees and good mechanical properties (excluding CSCA) compared with those of neat CS films. Additionally, every crosslinked CS film coated with thrombin (TB-CS) showed enhanced hemostatic ability in the whole blood clotting and activated partial thromboplastin time tests. Furthermore, the CSCA, CSGTA, and CSGP were nontoxic in an in vitro cell cytotoxicity test (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay) using L-929 mouse fibroblasts cells.
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spelling pubmed-83484212021-08-08 Hemostatic Patches Based on Crosslinked Chitosan Films Applied in Interventional Procedures Lee, Moon Hyun Lee, Dae Ryeong Chon, Joon Woo Chung, Dong June Polymers (Basel) Article In this study, we manufactured biocompatible hemostatic crosslinked chitosan (CS) patches and analyzed their physicochemical and biological properties for femoral arterial puncture applications. CS is a representative hemostatic material but has some drawbacks, such as swelling, shrinkage, and brittleness. Thus, it was crosslinked via a 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) coupling reaction and a nucleophilic addition reaction with citric acid (CA), glutaraldehyde (GTA), and genipin (GP) to remedy its shortcomings. The CSCA (crosslinked CS with CA/EDC), CSGTA (crosslinked CS with GTA), and CSG (crosslinked CS with GP) films showed low swelling degrees and good mechanical properties (excluding CSCA) compared with those of neat CS films. Additionally, every crosslinked CS film coated with thrombin (TB-CS) showed enhanced hemostatic ability in the whole blood clotting and activated partial thromboplastin time tests. Furthermore, the CSCA, CSGTA, and CSGP were nontoxic in an in vitro cell cytotoxicity test (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay) using L-929 mouse fibroblasts cells. MDPI 2021-07-22 /pmc/articles/PMC8348421/ /pubmed/34372004 http://dx.doi.org/10.3390/polym13152402 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Moon Hyun
Lee, Dae Ryeong
Chon, Joon Woo
Chung, Dong June
Hemostatic Patches Based on Crosslinked Chitosan Films Applied in Interventional Procedures
title Hemostatic Patches Based on Crosslinked Chitosan Films Applied in Interventional Procedures
title_full Hemostatic Patches Based on Crosslinked Chitosan Films Applied in Interventional Procedures
title_fullStr Hemostatic Patches Based on Crosslinked Chitosan Films Applied in Interventional Procedures
title_full_unstemmed Hemostatic Patches Based on Crosslinked Chitosan Films Applied in Interventional Procedures
title_short Hemostatic Patches Based on Crosslinked Chitosan Films Applied in Interventional Procedures
title_sort hemostatic patches based on crosslinked chitosan films applied in interventional procedures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348421/
https://www.ncbi.nlm.nih.gov/pubmed/34372004
http://dx.doi.org/10.3390/polym13152402
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