Cargando…
The Role of Caspase-12 in Retinal Bystander Cell Death and Innate Immune Responses against MCMV Retinitis
(1) Background: caspase-12 is activated during cytomegalovirus retinitis, although its role is presently unclear. (2) Methods: caspase-12(−/−) (KO) or caspase-12(+/+) (WT) mice were immunosup eyes were analyzed by plaque assay, TUNEL assay, immunohistochemical staining, western blotting, and real-ti...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348425/ https://www.ncbi.nlm.nih.gov/pubmed/34360899 http://dx.doi.org/10.3390/ijms22158135 |
_version_ | 1783735336838365184 |
---|---|
author | Zhang, Xinyan Xu, Jinxian Marshall, Brendan Dong, Zheng Smith, Sylvia B. Zhang, Ming |
author_facet | Zhang, Xinyan Xu, Jinxian Marshall, Brendan Dong, Zheng Smith, Sylvia B. Zhang, Ming |
author_sort | Zhang, Xinyan |
collection | PubMed |
description | (1) Background: caspase-12 is activated during cytomegalovirus retinitis, although its role is presently unclear. (2) Methods: caspase-12(−/−) (KO) or caspase-12(+/+) (WT) mice were immunosup eyes were analyzed by plaque assay, TUNEL assay, immunohistochemical staining, western blotting, and real-time PCR. (3) Results: increased retinitis and a more extensive virus spread were detected in the retina of infected eyes of KO mice compared to WT mice at day 14 p.i. Compared to MCMV injected WT eyes, mRNA levels of interferons α, β and γ were significantly reduced in the neural retina of MCMV-infected KO eyes at day 14 p.i. Although similar numbers of MCMV infected cells, similar virus titers and similar numbers of TUNEL-staining cells were detected in injected eyes of both KO and WT mice at days 7 and 10 p.i., significantly lower amounts of cleaved caspase-3 and p53 protein were detected in infected eyes of KO mice at both time points. (4) Conclusions: caspase-12 contributes to caspase-3-dependent and independent retinal bystander cell death during MCMV retinitis and may also play an important role in innate immunity against virus infection of the retina. |
format | Online Article Text |
id | pubmed-8348425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83484252021-08-08 The Role of Caspase-12 in Retinal Bystander Cell Death and Innate Immune Responses against MCMV Retinitis Zhang, Xinyan Xu, Jinxian Marshall, Brendan Dong, Zheng Smith, Sylvia B. Zhang, Ming Int J Mol Sci Article (1) Background: caspase-12 is activated during cytomegalovirus retinitis, although its role is presently unclear. (2) Methods: caspase-12(−/−) (KO) or caspase-12(+/+) (WT) mice were immunosup eyes were analyzed by plaque assay, TUNEL assay, immunohistochemical staining, western blotting, and real-time PCR. (3) Results: increased retinitis and a more extensive virus spread were detected in the retina of infected eyes of KO mice compared to WT mice at day 14 p.i. Compared to MCMV injected WT eyes, mRNA levels of interferons α, β and γ were significantly reduced in the neural retina of MCMV-infected KO eyes at day 14 p.i. Although similar numbers of MCMV infected cells, similar virus titers and similar numbers of TUNEL-staining cells were detected in injected eyes of both KO and WT mice at days 7 and 10 p.i., significantly lower amounts of cleaved caspase-3 and p53 protein were detected in infected eyes of KO mice at both time points. (4) Conclusions: caspase-12 contributes to caspase-3-dependent and independent retinal bystander cell death during MCMV retinitis and may also play an important role in innate immunity against virus infection of the retina. MDPI 2021-07-29 /pmc/articles/PMC8348425/ /pubmed/34360899 http://dx.doi.org/10.3390/ijms22158135 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Xinyan Xu, Jinxian Marshall, Brendan Dong, Zheng Smith, Sylvia B. Zhang, Ming The Role of Caspase-12 in Retinal Bystander Cell Death and Innate Immune Responses against MCMV Retinitis |
title | The Role of Caspase-12 in Retinal Bystander Cell Death and Innate Immune Responses against MCMV Retinitis |
title_full | The Role of Caspase-12 in Retinal Bystander Cell Death and Innate Immune Responses against MCMV Retinitis |
title_fullStr | The Role of Caspase-12 in Retinal Bystander Cell Death and Innate Immune Responses against MCMV Retinitis |
title_full_unstemmed | The Role of Caspase-12 in Retinal Bystander Cell Death and Innate Immune Responses against MCMV Retinitis |
title_short | The Role of Caspase-12 in Retinal Bystander Cell Death and Innate Immune Responses against MCMV Retinitis |
title_sort | role of caspase-12 in retinal bystander cell death and innate immune responses against mcmv retinitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348425/ https://www.ncbi.nlm.nih.gov/pubmed/34360899 http://dx.doi.org/10.3390/ijms22158135 |
work_keys_str_mv | AT zhangxinyan theroleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT xujinxian theroleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT marshallbrendan theroleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT dongzheng theroleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT smithsylviab theroleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT zhangming theroleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT zhangxinyan roleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT xujinxian roleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT marshallbrendan roleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT dongzheng roleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT smithsylviab roleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis AT zhangming roleofcaspase12inretinalbystandercelldeathandinnateimmuneresponsesagainstmcmvretinitis |