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Deferoxamine Reduces Inflammation and Osteoclastogenesis in Avulsed Teeth

Replacement and inflammatory resorption are serious complications associated with the delayed replantation of avulsed teeth. In this study, we aimed to assess whether deferoxamine (DFO) can suppress inflammation and osteoclastogenesis in vitro and attenuate inflammation and bone resorption in a repl...

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Autores principales: Lee, Ko Eun, Mo, Seunghan, Lee, Hyo-Seol, Jeon, Mijeong, Song, Je Seon, Choi, Hyung-Jun, Cho, Hyunsoo, Kang, Chung-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348439/
https://www.ncbi.nlm.nih.gov/pubmed/34360988
http://dx.doi.org/10.3390/ijms22158225
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author Lee, Ko Eun
Mo, Seunghan
Lee, Hyo-Seol
Jeon, Mijeong
Song, Je Seon
Choi, Hyung-Jun
Cho, Hyunsoo
Kang, Chung-Min
author_facet Lee, Ko Eun
Mo, Seunghan
Lee, Hyo-Seol
Jeon, Mijeong
Song, Je Seon
Choi, Hyung-Jun
Cho, Hyunsoo
Kang, Chung-Min
author_sort Lee, Ko Eun
collection PubMed
description Replacement and inflammatory resorption are serious complications associated with the delayed replantation of avulsed teeth. In this study, we aimed to assess whether deferoxamine (DFO) can suppress inflammation and osteoclastogenesis in vitro and attenuate inflammation and bone resorption in a replanted rat tooth model. Cell viability and inflammation were evaluated in RAW264.7 cells. Osteoclastogenesis was confirmed by tartrate-resistant acid phosphatase staining, reactive oxygen species (ROS) measurement, and quantitative reverse transcriptase–polymerase chain reaction in teeth exposed to different concentrations of DFO. In vivo, molars of 31 six-week-old male Sprague–Dawley rats were extracted and stored in saline (n = 10) or DFO solution (n = 21) before replantation. Micro-computed tomography (micro-CT) imaging and histological analysis were performed to evaluate inflammation and root and alveolar bone resorption. DFO downregulated the genes related to inflammation and osteoclastogenesis. DFO also reduced ROS production and regulated specific pathways. Furthermore, the results of the micro-CT and histological analyses provided evidence of the decrease in inflammation and hard tissue resorption in the DFO group. Overall, these results suggest that DFO reduces inflammation and osteoclastogenesis in a tooth replantation model, and thus, it has to be further investigated as a root surface treatment option for an avulsed tooth.
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spelling pubmed-83484392021-08-08 Deferoxamine Reduces Inflammation and Osteoclastogenesis in Avulsed Teeth Lee, Ko Eun Mo, Seunghan Lee, Hyo-Seol Jeon, Mijeong Song, Je Seon Choi, Hyung-Jun Cho, Hyunsoo Kang, Chung-Min Int J Mol Sci Article Replacement and inflammatory resorption are serious complications associated with the delayed replantation of avulsed teeth. In this study, we aimed to assess whether deferoxamine (DFO) can suppress inflammation and osteoclastogenesis in vitro and attenuate inflammation and bone resorption in a replanted rat tooth model. Cell viability and inflammation were evaluated in RAW264.7 cells. Osteoclastogenesis was confirmed by tartrate-resistant acid phosphatase staining, reactive oxygen species (ROS) measurement, and quantitative reverse transcriptase–polymerase chain reaction in teeth exposed to different concentrations of DFO. In vivo, molars of 31 six-week-old male Sprague–Dawley rats were extracted and stored in saline (n = 10) or DFO solution (n = 21) before replantation. Micro-computed tomography (micro-CT) imaging and histological analysis were performed to evaluate inflammation and root and alveolar bone resorption. DFO downregulated the genes related to inflammation and osteoclastogenesis. DFO also reduced ROS production and regulated specific pathways. Furthermore, the results of the micro-CT and histological analyses provided evidence of the decrease in inflammation and hard tissue resorption in the DFO group. Overall, these results suggest that DFO reduces inflammation and osteoclastogenesis in a tooth replantation model, and thus, it has to be further investigated as a root surface treatment option for an avulsed tooth. MDPI 2021-07-30 /pmc/articles/PMC8348439/ /pubmed/34360988 http://dx.doi.org/10.3390/ijms22158225 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Ko Eun
Mo, Seunghan
Lee, Hyo-Seol
Jeon, Mijeong
Song, Je Seon
Choi, Hyung-Jun
Cho, Hyunsoo
Kang, Chung-Min
Deferoxamine Reduces Inflammation and Osteoclastogenesis in Avulsed Teeth
title Deferoxamine Reduces Inflammation and Osteoclastogenesis in Avulsed Teeth
title_full Deferoxamine Reduces Inflammation and Osteoclastogenesis in Avulsed Teeth
title_fullStr Deferoxamine Reduces Inflammation and Osteoclastogenesis in Avulsed Teeth
title_full_unstemmed Deferoxamine Reduces Inflammation and Osteoclastogenesis in Avulsed Teeth
title_short Deferoxamine Reduces Inflammation and Osteoclastogenesis in Avulsed Teeth
title_sort deferoxamine reduces inflammation and osteoclastogenesis in avulsed teeth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348439/
https://www.ncbi.nlm.nih.gov/pubmed/34360988
http://dx.doi.org/10.3390/ijms22158225
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