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Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments
Phenolic structures are of great interest due to their antioxidant properties and various postulated benefits on human health. However, the quantification of these structures in fruits and vegetables, as well as in vivo or in vitro experiments, is demanding, as relevant concentrations are often low,...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348453/ https://www.ncbi.nlm.nih.gov/pubmed/34361808 http://dx.doi.org/10.3390/molecules26154656 |
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author | Kaeswurm, Julia A. H. Scharinger, Andreas Teipel, Jan Buchweitz, Maria |
author_facet | Kaeswurm, Julia A. H. Scharinger, Andreas Teipel, Jan Buchweitz, Maria |
author_sort | Kaeswurm, Julia A. H. |
collection | PubMed |
description | Phenolic structures are of great interest due to their antioxidant properties and various postulated benefits on human health. However, the quantification of these structures in fruits and vegetables, as well as in vivo or in vitro experiments, is demanding, as relevant concentrations are often low, causing problems in exactly weighing the respective amounts. Nevertheless, the determination of used concentrations is often a prerequisite for accurate results. A possibility to quantify polyphenol is the use of UV/vis spectroscopy. Therefore, the absorption coefficients of selected phenolic structures were determined in three different solvents relevant for polyphenol research (water/methanol (50/50, v/v), water, and phosphate buffer at pH 7.5). To confirm the values based on weight and to avoid errors due to impurities, hygroscopic effects, and inadequate balance care, the mass concentrations were additionally determined by quantitative NMR (q-NMR). The coefficients presented in this article can help to quickly and easily determine accurate concentrations in a laboratory routine without wasting the often-precious standard compounds. |
format | Online Article Text |
id | pubmed-8348453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83484532021-08-08 Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments Kaeswurm, Julia A. H. Scharinger, Andreas Teipel, Jan Buchweitz, Maria Molecules Article Phenolic structures are of great interest due to their antioxidant properties and various postulated benefits on human health. However, the quantification of these structures in fruits and vegetables, as well as in vivo or in vitro experiments, is demanding, as relevant concentrations are often low, causing problems in exactly weighing the respective amounts. Nevertheless, the determination of used concentrations is often a prerequisite for accurate results. A possibility to quantify polyphenol is the use of UV/vis spectroscopy. Therefore, the absorption coefficients of selected phenolic structures were determined in three different solvents relevant for polyphenol research (water/methanol (50/50, v/v), water, and phosphate buffer at pH 7.5). To confirm the values based on weight and to avoid errors due to impurities, hygroscopic effects, and inadequate balance care, the mass concentrations were additionally determined by quantitative NMR (q-NMR). The coefficients presented in this article can help to quickly and easily determine accurate concentrations in a laboratory routine without wasting the often-precious standard compounds. MDPI 2021-07-31 /pmc/articles/PMC8348453/ /pubmed/34361808 http://dx.doi.org/10.3390/molecules26154656 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kaeswurm, Julia A. H. Scharinger, Andreas Teipel, Jan Buchweitz, Maria Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments |
title | Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments |
title_full | Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments |
title_fullStr | Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments |
title_full_unstemmed | Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments |
title_short | Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments |
title_sort | absorption coefficients of phenolic structures in different solvents routinely used for experiments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348453/ https://www.ncbi.nlm.nih.gov/pubmed/34361808 http://dx.doi.org/10.3390/molecules26154656 |
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