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Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments

Phenolic structures are of great interest due to their antioxidant properties and various postulated benefits on human health. However, the quantification of these structures in fruits and vegetables, as well as in vivo or in vitro experiments, is demanding, as relevant concentrations are often low,...

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Autores principales: Kaeswurm, Julia A. H., Scharinger, Andreas, Teipel, Jan, Buchweitz, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348453/
https://www.ncbi.nlm.nih.gov/pubmed/34361808
http://dx.doi.org/10.3390/molecules26154656
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author Kaeswurm, Julia A. H.
Scharinger, Andreas
Teipel, Jan
Buchweitz, Maria
author_facet Kaeswurm, Julia A. H.
Scharinger, Andreas
Teipel, Jan
Buchweitz, Maria
author_sort Kaeswurm, Julia A. H.
collection PubMed
description Phenolic structures are of great interest due to their antioxidant properties and various postulated benefits on human health. However, the quantification of these structures in fruits and vegetables, as well as in vivo or in vitro experiments, is demanding, as relevant concentrations are often low, causing problems in exactly weighing the respective amounts. Nevertheless, the determination of used concentrations is often a prerequisite for accurate results. A possibility to quantify polyphenol is the use of UV/vis spectroscopy. Therefore, the absorption coefficients of selected phenolic structures were determined in three different solvents relevant for polyphenol research (water/methanol (50/50, v/v), water, and phosphate buffer at pH 7.5). To confirm the values based on weight and to avoid errors due to impurities, hygroscopic effects, and inadequate balance care, the mass concentrations were additionally determined by quantitative NMR (q-NMR). The coefficients presented in this article can help to quickly and easily determine accurate concentrations in a laboratory routine without wasting the often-precious standard compounds.
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spelling pubmed-83484532021-08-08 Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments Kaeswurm, Julia A. H. Scharinger, Andreas Teipel, Jan Buchweitz, Maria Molecules Article Phenolic structures are of great interest due to their antioxidant properties and various postulated benefits on human health. However, the quantification of these structures in fruits and vegetables, as well as in vivo or in vitro experiments, is demanding, as relevant concentrations are often low, causing problems in exactly weighing the respective amounts. Nevertheless, the determination of used concentrations is often a prerequisite for accurate results. A possibility to quantify polyphenol is the use of UV/vis spectroscopy. Therefore, the absorption coefficients of selected phenolic structures were determined in three different solvents relevant for polyphenol research (water/methanol (50/50, v/v), water, and phosphate buffer at pH 7.5). To confirm the values based on weight and to avoid errors due to impurities, hygroscopic effects, and inadequate balance care, the mass concentrations were additionally determined by quantitative NMR (q-NMR). The coefficients presented in this article can help to quickly and easily determine accurate concentrations in a laboratory routine without wasting the often-precious standard compounds. MDPI 2021-07-31 /pmc/articles/PMC8348453/ /pubmed/34361808 http://dx.doi.org/10.3390/molecules26154656 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kaeswurm, Julia A. H.
Scharinger, Andreas
Teipel, Jan
Buchweitz, Maria
Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments
title Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments
title_full Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments
title_fullStr Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments
title_full_unstemmed Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments
title_short Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments
title_sort absorption coefficients of phenolic structures in different solvents routinely used for experiments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348453/
https://www.ncbi.nlm.nih.gov/pubmed/34361808
http://dx.doi.org/10.3390/molecules26154656
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