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Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography

The market of biomolecules with therapeutic scopes, including peptides, is continuously expanding. The interest towards this class of pharmaceuticals is stimulated by the broad range of bioactivities that peptides can trigger in the human body. The main production methods to obtain peptides are enzy...

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Autores principales: De Luca, Chiara, Lievore, Giulio, Bozza, Desiree, Buratti, Alessandro, Cavazzini, Alberto, Ricci, Antonio, Macis, Marco, Cabri, Walter, Felletti, Simona, Catani, Martina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348516/
https://www.ncbi.nlm.nih.gov/pubmed/34361839
http://dx.doi.org/10.3390/molecules26154688
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author De Luca, Chiara
Lievore, Giulio
Bozza, Desiree
Buratti, Alessandro
Cavazzini, Alberto
Ricci, Antonio
Macis, Marco
Cabri, Walter
Felletti, Simona
Catani, Martina
author_facet De Luca, Chiara
Lievore, Giulio
Bozza, Desiree
Buratti, Alessandro
Cavazzini, Alberto
Ricci, Antonio
Macis, Marco
Cabri, Walter
Felletti, Simona
Catani, Martina
author_sort De Luca, Chiara
collection PubMed
description The market of biomolecules with therapeutic scopes, including peptides, is continuously expanding. The interest towards this class of pharmaceuticals is stimulated by the broad range of bioactivities that peptides can trigger in the human body. The main production methods to obtain peptides are enzymatic hydrolysis, microbial fermentation, recombinant approach and, especially, chemical synthesis. None of these methods, however, produce exclusively the target product. Other species represent impurities that, for safety and pharmaceutical quality reasons, must be removed. The remarkable production volumes of peptide mixtures have generated a strong interest towards the purification procedures, particularly due to their relevant impact on the manufacturing costs. The purification method of choice is mainly preparative liquid chromatography, because of its flexibility, which allows one to choose case-by-case the experimental conditions that most suitably fit that particular purification problem. Different modes of chromatography that can cover almost every separation case are reviewed in this article. Additionally, an outlook to a very recent continuous chromatographic process (namely Multicolumn Countercurrent Solvent Gradient Purification, MCSGP) and future perspectives regarding purification strategies will be considered at the end of this review.
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spelling pubmed-83485162021-08-08 Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography De Luca, Chiara Lievore, Giulio Bozza, Desiree Buratti, Alessandro Cavazzini, Alberto Ricci, Antonio Macis, Marco Cabri, Walter Felletti, Simona Catani, Martina Molecules Review The market of biomolecules with therapeutic scopes, including peptides, is continuously expanding. The interest towards this class of pharmaceuticals is stimulated by the broad range of bioactivities that peptides can trigger in the human body. The main production methods to obtain peptides are enzymatic hydrolysis, microbial fermentation, recombinant approach and, especially, chemical synthesis. None of these methods, however, produce exclusively the target product. Other species represent impurities that, for safety and pharmaceutical quality reasons, must be removed. The remarkable production volumes of peptide mixtures have generated a strong interest towards the purification procedures, particularly due to their relevant impact on the manufacturing costs. The purification method of choice is mainly preparative liquid chromatography, because of its flexibility, which allows one to choose case-by-case the experimental conditions that most suitably fit that particular purification problem. Different modes of chromatography that can cover almost every separation case are reviewed in this article. Additionally, an outlook to a very recent continuous chromatographic process (namely Multicolumn Countercurrent Solvent Gradient Purification, MCSGP) and future perspectives regarding purification strategies will be considered at the end of this review. MDPI 2021-08-03 /pmc/articles/PMC8348516/ /pubmed/34361839 http://dx.doi.org/10.3390/molecules26154688 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
De Luca, Chiara
Lievore, Giulio
Bozza, Desiree
Buratti, Alessandro
Cavazzini, Alberto
Ricci, Antonio
Macis, Marco
Cabri, Walter
Felletti, Simona
Catani, Martina
Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography
title Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography
title_full Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography
title_fullStr Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography
title_full_unstemmed Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography
title_short Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography
title_sort downstream processing of therapeutic peptides by means of preparative liquid chromatography
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348516/
https://www.ncbi.nlm.nih.gov/pubmed/34361839
http://dx.doi.org/10.3390/molecules26154688
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