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Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line

The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and ex...

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Autores principales: Venkatachalapathy, Deepthi, Shivamallu, Chandan, Prasad, Shashanka K., Thangaraj Saradha, Gopenath, Rudrapathy, Parthiban, Amachawadi, Raghavendra G., Patil, Sharanagouda S., Syed, Asad, Elgorban, Abdallah M., Bahkali, Ali H., Kollur, Shiva Prasad, Basalingappa, Kanthesh M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348645/
https://www.ncbi.nlm.nih.gov/pubmed/34361745
http://dx.doi.org/10.3390/molecules26154593
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author Venkatachalapathy, Deepthi
Shivamallu, Chandan
Prasad, Shashanka K.
Thangaraj Saradha, Gopenath
Rudrapathy, Parthiban
Amachawadi, Raghavendra G.
Patil, Sharanagouda S.
Syed, Asad
Elgorban, Abdallah M.
Bahkali, Ali H.
Kollur, Shiva Prasad
Basalingappa, Kanthesh M.
author_facet Venkatachalapathy, Deepthi
Shivamallu, Chandan
Prasad, Shashanka K.
Thangaraj Saradha, Gopenath
Rudrapathy, Parthiban
Amachawadi, Raghavendra G.
Patil, Sharanagouda S.
Syed, Asad
Elgorban, Abdallah M.
Bahkali, Ali H.
Kollur, Shiva Prasad
Basalingappa, Kanthesh M.
author_sort Venkatachalapathy, Deepthi
collection PubMed
description The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and exhibit anti-carcinogenic properties. This study aims to evaluate the anti-cancerous properties of these plant crude extracts on human liver cancer HepG2 cells. The leaves of Camellia sinensis, Withania somnifera and the seeds of Vitis vinifera were collected and methanolic extracts were prepared. Then, these extracts were subjected to DPPH, α- amylase assays to determine the antioxidant properties. A MTT assay was performed to investigate the viability of the extracts of HepG2 cells, and the mode of cell death was detected by Ao/EtBr staining and flow cytometry with PI Annexin- V FITC dual staining. Then, the protein expression of BAX and BCl2 was studied using fluorescent dye to determine the regulation of the BAX and BCl2 genes. We observed that all the three extracts showed the presence of bioactive compounds such as polyphenols or phytochemicals. The W. somnifera bioactive compounds were found to have the highest anti-proliferative activity on human liver cancer cells.
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spelling pubmed-83486452021-08-08 Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line Venkatachalapathy, Deepthi Shivamallu, Chandan Prasad, Shashanka K. Thangaraj Saradha, Gopenath Rudrapathy, Parthiban Amachawadi, Raghavendra G. Patil, Sharanagouda S. Syed, Asad Elgorban, Abdallah M. Bahkali, Ali H. Kollur, Shiva Prasad Basalingappa, Kanthesh M. Molecules Article The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and exhibit anti-carcinogenic properties. This study aims to evaluate the anti-cancerous properties of these plant crude extracts on human liver cancer HepG2 cells. The leaves of Camellia sinensis, Withania somnifera and the seeds of Vitis vinifera were collected and methanolic extracts were prepared. Then, these extracts were subjected to DPPH, α- amylase assays to determine the antioxidant properties. A MTT assay was performed to investigate the viability of the extracts of HepG2 cells, and the mode of cell death was detected by Ao/EtBr staining and flow cytometry with PI Annexin- V FITC dual staining. Then, the protein expression of BAX and BCl2 was studied using fluorescent dye to determine the regulation of the BAX and BCl2 genes. We observed that all the three extracts showed the presence of bioactive compounds such as polyphenols or phytochemicals. The W. somnifera bioactive compounds were found to have the highest anti-proliferative activity on human liver cancer cells. MDPI 2021-07-29 /pmc/articles/PMC8348645/ /pubmed/34361745 http://dx.doi.org/10.3390/molecules26154593 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Venkatachalapathy, Deepthi
Shivamallu, Chandan
Prasad, Shashanka K.
Thangaraj Saradha, Gopenath
Rudrapathy, Parthiban
Amachawadi, Raghavendra G.
Patil, Sharanagouda S.
Syed, Asad
Elgorban, Abdallah M.
Bahkali, Ali H.
Kollur, Shiva Prasad
Basalingappa, Kanthesh M.
Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line
title Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line
title_full Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line
title_fullStr Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line
title_full_unstemmed Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line
title_short Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line
title_sort assessment of chemopreventive potential of the plant extracts against liver cancer using hepg2 cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348645/
https://www.ncbi.nlm.nih.gov/pubmed/34361745
http://dx.doi.org/10.3390/molecules26154593
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