Cargando…
Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line
The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and ex...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348645/ https://www.ncbi.nlm.nih.gov/pubmed/34361745 http://dx.doi.org/10.3390/molecules26154593 |
_version_ | 1783735390492950528 |
---|---|
author | Venkatachalapathy, Deepthi Shivamallu, Chandan Prasad, Shashanka K. Thangaraj Saradha, Gopenath Rudrapathy, Parthiban Amachawadi, Raghavendra G. Patil, Sharanagouda S. Syed, Asad Elgorban, Abdallah M. Bahkali, Ali H. Kollur, Shiva Prasad Basalingappa, Kanthesh M. |
author_facet | Venkatachalapathy, Deepthi Shivamallu, Chandan Prasad, Shashanka K. Thangaraj Saradha, Gopenath Rudrapathy, Parthiban Amachawadi, Raghavendra G. Patil, Sharanagouda S. Syed, Asad Elgorban, Abdallah M. Bahkali, Ali H. Kollur, Shiva Prasad Basalingappa, Kanthesh M. |
author_sort | Venkatachalapathy, Deepthi |
collection | PubMed |
description | The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and exhibit anti-carcinogenic properties. This study aims to evaluate the anti-cancerous properties of these plant crude extracts on human liver cancer HepG2 cells. The leaves of Camellia sinensis, Withania somnifera and the seeds of Vitis vinifera were collected and methanolic extracts were prepared. Then, these extracts were subjected to DPPH, α- amylase assays to determine the antioxidant properties. A MTT assay was performed to investigate the viability of the extracts of HepG2 cells, and the mode of cell death was detected by Ao/EtBr staining and flow cytometry with PI Annexin- V FITC dual staining. Then, the protein expression of BAX and BCl2 was studied using fluorescent dye to determine the regulation of the BAX and BCl2 genes. We observed that all the three extracts showed the presence of bioactive compounds such as polyphenols or phytochemicals. The W. somnifera bioactive compounds were found to have the highest anti-proliferative activity on human liver cancer cells. |
format | Online Article Text |
id | pubmed-8348645 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83486452021-08-08 Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line Venkatachalapathy, Deepthi Shivamallu, Chandan Prasad, Shashanka K. Thangaraj Saradha, Gopenath Rudrapathy, Parthiban Amachawadi, Raghavendra G. Patil, Sharanagouda S. Syed, Asad Elgorban, Abdallah M. Bahkali, Ali H. Kollur, Shiva Prasad Basalingappa, Kanthesh M. Molecules Article The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and exhibit anti-carcinogenic properties. This study aims to evaluate the anti-cancerous properties of these plant crude extracts on human liver cancer HepG2 cells. The leaves of Camellia sinensis, Withania somnifera and the seeds of Vitis vinifera were collected and methanolic extracts were prepared. Then, these extracts were subjected to DPPH, α- amylase assays to determine the antioxidant properties. A MTT assay was performed to investigate the viability of the extracts of HepG2 cells, and the mode of cell death was detected by Ao/EtBr staining and flow cytometry with PI Annexin- V FITC dual staining. Then, the protein expression of BAX and BCl2 was studied using fluorescent dye to determine the regulation of the BAX and BCl2 genes. We observed that all the three extracts showed the presence of bioactive compounds such as polyphenols or phytochemicals. The W. somnifera bioactive compounds were found to have the highest anti-proliferative activity on human liver cancer cells. MDPI 2021-07-29 /pmc/articles/PMC8348645/ /pubmed/34361745 http://dx.doi.org/10.3390/molecules26154593 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Venkatachalapathy, Deepthi Shivamallu, Chandan Prasad, Shashanka K. Thangaraj Saradha, Gopenath Rudrapathy, Parthiban Amachawadi, Raghavendra G. Patil, Sharanagouda S. Syed, Asad Elgorban, Abdallah M. Bahkali, Ali H. Kollur, Shiva Prasad Basalingappa, Kanthesh M. Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line |
title | Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line |
title_full | Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line |
title_fullStr | Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line |
title_full_unstemmed | Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line |
title_short | Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line |
title_sort | assessment of chemopreventive potential of the plant extracts against liver cancer using hepg2 cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348645/ https://www.ncbi.nlm.nih.gov/pubmed/34361745 http://dx.doi.org/10.3390/molecules26154593 |
work_keys_str_mv | AT venkatachalapathydeepthi assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT shivamalluchandan assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT prasadshashankak assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT thangarajsaradhagopenath assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT rudrapathyparthiban assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT amachawadiraghavendrag assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT patilsharanagoudas assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT syedasad assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT elgorbanabdallahm assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT bahkalialih assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT kollurshivaprasad assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline AT basalingappakantheshm assessmentofchemopreventivepotentialoftheplantextractsagainstlivercancerusinghepg2cellline |