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3D-Printable Hierarchical Nanogel-GelMA Composite Hydrogel System

The strength of the extracellular matrix (ECM) is that it is hierarchical in terms of matrix built-up, matrix density and fiber structure, which allows for hormones, cytokines, and other small biomolecules to be stored within its network. The ECM-like hydrogels that are currently used do not possess...

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Autores principales: Zu, Guangyue, Meijer, Marnix, Mergel, Olga, Zhang, Heng, van Rijn, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348806/
https://www.ncbi.nlm.nih.gov/pubmed/34372111
http://dx.doi.org/10.3390/polym13152508
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author Zu, Guangyue
Meijer, Marnix
Mergel, Olga
Zhang, Heng
van Rijn, Patrick
author_facet Zu, Guangyue
Meijer, Marnix
Mergel, Olga
Zhang, Heng
van Rijn, Patrick
author_sort Zu, Guangyue
collection PubMed
description The strength of the extracellular matrix (ECM) is that it is hierarchical in terms of matrix built-up, matrix density and fiber structure, which allows for hormones, cytokines, and other small biomolecules to be stored within its network. The ECM-like hydrogels that are currently used do not possess this ability, and long-term storage, along with the need for free diffusion of small molecules, are generally incompatible requirements. Nanogels are able to fulfill the additional requirements upon successful integration. Herein, a stable hierarchical nanogel–gelatin methacryloyl (GelMA) composite hydrogel system is provided by covalently embedding nanogels inside the micropore network of GelMA hydrogel to allow a controlled local functionality that is not found in a homogenous GelMA hydrogel. Nanogels have emerged as a powerful tool in nanomedicine and are highly versatile, due to their simplicity of chemical control and biological compatibility. In this study, an N-isopropylacrylamide-based nanogel with primary amine groups on the surface was modified with methacryloyl groups to obtain a photo-cross-linking ability similar to GelMA. The nanogel-GelMA composite hydrogel was formed by mixing the GelMA and the photo-initiator within the nanogel solution through UV irradiation. The morphology of the composite hydrogel was observed by scanning electron microscopy, which clearly showed the nanogel wrapped within the GelMA network and covering the surface of the pore wall. A release experiment was conducted to prove covalent bonding and the stability of the nanogel inside the GelMA hydrogel. In addition, 3D printability studies showed that the nanogel-GelMA composite ink is printable. Therefore, the suggested stable hierarchical nanogel-GelMA composite hydrogel system has great potential to achieve the in situ delivery and controllable release of bioactive molecules in 3D cell culture systems.
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spelling pubmed-83488062021-08-08 3D-Printable Hierarchical Nanogel-GelMA Composite Hydrogel System Zu, Guangyue Meijer, Marnix Mergel, Olga Zhang, Heng van Rijn, Patrick Polymers (Basel) Article The strength of the extracellular matrix (ECM) is that it is hierarchical in terms of matrix built-up, matrix density and fiber structure, which allows for hormones, cytokines, and other small biomolecules to be stored within its network. The ECM-like hydrogels that are currently used do not possess this ability, and long-term storage, along with the need for free diffusion of small molecules, are generally incompatible requirements. Nanogels are able to fulfill the additional requirements upon successful integration. Herein, a stable hierarchical nanogel–gelatin methacryloyl (GelMA) composite hydrogel system is provided by covalently embedding nanogels inside the micropore network of GelMA hydrogel to allow a controlled local functionality that is not found in a homogenous GelMA hydrogel. Nanogels have emerged as a powerful tool in nanomedicine and are highly versatile, due to their simplicity of chemical control and biological compatibility. In this study, an N-isopropylacrylamide-based nanogel with primary amine groups on the surface was modified with methacryloyl groups to obtain a photo-cross-linking ability similar to GelMA. The nanogel-GelMA composite hydrogel was formed by mixing the GelMA and the photo-initiator within the nanogel solution through UV irradiation. The morphology of the composite hydrogel was observed by scanning electron microscopy, which clearly showed the nanogel wrapped within the GelMA network and covering the surface of the pore wall. A release experiment was conducted to prove covalent bonding and the stability of the nanogel inside the GelMA hydrogel. In addition, 3D printability studies showed that the nanogel-GelMA composite ink is printable. Therefore, the suggested stable hierarchical nanogel-GelMA composite hydrogel system has great potential to achieve the in situ delivery and controllable release of bioactive molecules in 3D cell culture systems. MDPI 2021-07-29 /pmc/articles/PMC8348806/ /pubmed/34372111 http://dx.doi.org/10.3390/polym13152508 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zu, Guangyue
Meijer, Marnix
Mergel, Olga
Zhang, Heng
van Rijn, Patrick
3D-Printable Hierarchical Nanogel-GelMA Composite Hydrogel System
title 3D-Printable Hierarchical Nanogel-GelMA Composite Hydrogel System
title_full 3D-Printable Hierarchical Nanogel-GelMA Composite Hydrogel System
title_fullStr 3D-Printable Hierarchical Nanogel-GelMA Composite Hydrogel System
title_full_unstemmed 3D-Printable Hierarchical Nanogel-GelMA Composite Hydrogel System
title_short 3D-Printable Hierarchical Nanogel-GelMA Composite Hydrogel System
title_sort 3d-printable hierarchical nanogel-gelma composite hydrogel system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348806/
https://www.ncbi.nlm.nih.gov/pubmed/34372111
http://dx.doi.org/10.3390/polym13152508
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