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Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs
Virus-like particles are excellent inducers of the adaptive immune response of humans and are presently being used as scaffolds for the presentation of foreign peptides and antigens derived from infectious microorganisms for subunit vaccine development. The most common approaches for peptide and ant...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348948/ https://www.ncbi.nlm.nih.gov/pubmed/34361589 http://dx.doi.org/10.3390/molecules26154436 |
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author | Zapata-Cuellar, Lorena Gaona-Bernal, Jorge Manuel-Cabrera, Carlos Alberto Martínez-Velázquez, Moisés Sánchez-Hernández, Carla Elizondo-Quiroga, Darwin Camacho-Villegas, Tanya Amanda Gutiérrez-Ortega, Abel |
author_facet | Zapata-Cuellar, Lorena Gaona-Bernal, Jorge Manuel-Cabrera, Carlos Alberto Martínez-Velázquez, Moisés Sánchez-Hernández, Carla Elizondo-Quiroga, Darwin Camacho-Villegas, Tanya Amanda Gutiérrez-Ortega, Abel |
author_sort | Zapata-Cuellar, Lorena |
collection | PubMed |
description | Virus-like particles are excellent inducers of the adaptive immune response of humans and are presently being used as scaffolds for the presentation of foreign peptides and antigens derived from infectious microorganisms for subunit vaccine development. The most common approaches for peptide and antigen presentation are translational fusions and chemical coupling, but some alternatives that seek to simplify the coupling process have been reported recently. In this work, an alternative platform for coupling full antigens to virus-like particles is presented. Heterodimerization motifs inserted in both Tobacco etch virus coat protein and green fluorescent protein directed the coupling process by simple mixing, and the obtained complexes were easily taken up by a macrophage cell line. |
format | Online Article Text |
id | pubmed-8348948 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-83489482021-08-08 Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs Zapata-Cuellar, Lorena Gaona-Bernal, Jorge Manuel-Cabrera, Carlos Alberto Martínez-Velázquez, Moisés Sánchez-Hernández, Carla Elizondo-Quiroga, Darwin Camacho-Villegas, Tanya Amanda Gutiérrez-Ortega, Abel Molecules Article Virus-like particles are excellent inducers of the adaptive immune response of humans and are presently being used as scaffolds for the presentation of foreign peptides and antigens derived from infectious microorganisms for subunit vaccine development. The most common approaches for peptide and antigen presentation are translational fusions and chemical coupling, but some alternatives that seek to simplify the coupling process have been reported recently. In this work, an alternative platform for coupling full antigens to virus-like particles is presented. Heterodimerization motifs inserted in both Tobacco etch virus coat protein and green fluorescent protein directed the coupling process by simple mixing, and the obtained complexes were easily taken up by a macrophage cell line. MDPI 2021-07-23 /pmc/articles/PMC8348948/ /pubmed/34361589 http://dx.doi.org/10.3390/molecules26154436 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zapata-Cuellar, Lorena Gaona-Bernal, Jorge Manuel-Cabrera, Carlos Alberto Martínez-Velázquez, Moisés Sánchez-Hernández, Carla Elizondo-Quiroga, Darwin Camacho-Villegas, Tanya Amanda Gutiérrez-Ortega, Abel Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs |
title | Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs |
title_full | Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs |
title_fullStr | Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs |
title_full_unstemmed | Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs |
title_short | Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs |
title_sort | development of a platform for noncovalent coupling of full antigens to tobacco etch virus-like particles by means of coiled-coil oligomerization motifs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348948/ https://www.ncbi.nlm.nih.gov/pubmed/34361589 http://dx.doi.org/10.3390/molecules26154436 |
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