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Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs

Virus-like particles are excellent inducers of the adaptive immune response of humans and are presently being used as scaffolds for the presentation of foreign peptides and antigens derived from infectious microorganisms for subunit vaccine development. The most common approaches for peptide and ant...

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Detalles Bibliográficos
Autores principales: Zapata-Cuellar, Lorena, Gaona-Bernal, Jorge, Manuel-Cabrera, Carlos Alberto, Martínez-Velázquez, Moisés, Sánchez-Hernández, Carla, Elizondo-Quiroga, Darwin, Camacho-Villegas, Tanya Amanda, Gutiérrez-Ortega, Abel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348948/
https://www.ncbi.nlm.nih.gov/pubmed/34361589
http://dx.doi.org/10.3390/molecules26154436
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author Zapata-Cuellar, Lorena
Gaona-Bernal, Jorge
Manuel-Cabrera, Carlos Alberto
Martínez-Velázquez, Moisés
Sánchez-Hernández, Carla
Elizondo-Quiroga, Darwin
Camacho-Villegas, Tanya Amanda
Gutiérrez-Ortega, Abel
author_facet Zapata-Cuellar, Lorena
Gaona-Bernal, Jorge
Manuel-Cabrera, Carlos Alberto
Martínez-Velázquez, Moisés
Sánchez-Hernández, Carla
Elizondo-Quiroga, Darwin
Camacho-Villegas, Tanya Amanda
Gutiérrez-Ortega, Abel
author_sort Zapata-Cuellar, Lorena
collection PubMed
description Virus-like particles are excellent inducers of the adaptive immune response of humans and are presently being used as scaffolds for the presentation of foreign peptides and antigens derived from infectious microorganisms for subunit vaccine development. The most common approaches for peptide and antigen presentation are translational fusions and chemical coupling, but some alternatives that seek to simplify the coupling process have been reported recently. In this work, an alternative platform for coupling full antigens to virus-like particles is presented. Heterodimerization motifs inserted in both Tobacco etch virus coat protein and green fluorescent protein directed the coupling process by simple mixing, and the obtained complexes were easily taken up by a macrophage cell line.
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spelling pubmed-83489482021-08-08 Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs Zapata-Cuellar, Lorena Gaona-Bernal, Jorge Manuel-Cabrera, Carlos Alberto Martínez-Velázquez, Moisés Sánchez-Hernández, Carla Elizondo-Quiroga, Darwin Camacho-Villegas, Tanya Amanda Gutiérrez-Ortega, Abel Molecules Article Virus-like particles are excellent inducers of the adaptive immune response of humans and are presently being used as scaffolds for the presentation of foreign peptides and antigens derived from infectious microorganisms for subunit vaccine development. The most common approaches for peptide and antigen presentation are translational fusions and chemical coupling, but some alternatives that seek to simplify the coupling process have been reported recently. In this work, an alternative platform for coupling full antigens to virus-like particles is presented. Heterodimerization motifs inserted in both Tobacco etch virus coat protein and green fluorescent protein directed the coupling process by simple mixing, and the obtained complexes were easily taken up by a macrophage cell line. MDPI 2021-07-23 /pmc/articles/PMC8348948/ /pubmed/34361589 http://dx.doi.org/10.3390/molecules26154436 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zapata-Cuellar, Lorena
Gaona-Bernal, Jorge
Manuel-Cabrera, Carlos Alberto
Martínez-Velázquez, Moisés
Sánchez-Hernández, Carla
Elizondo-Quiroga, Darwin
Camacho-Villegas, Tanya Amanda
Gutiérrez-Ortega, Abel
Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs
title Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs
title_full Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs
title_fullStr Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs
title_full_unstemmed Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs
title_short Development of a Platform for Noncovalent Coupling of Full Antigens to Tobacco Etch Virus-Like Particles by Means of Coiled-Coil Oligomerization Motifs
title_sort development of a platform for noncovalent coupling of full antigens to tobacco etch virus-like particles by means of coiled-coil oligomerization motifs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348948/
https://www.ncbi.nlm.nih.gov/pubmed/34361589
http://dx.doi.org/10.3390/molecules26154436
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