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The correlation between ATP measurement and microbial contamination of inanimate surfaces
BACKGROUND: The objective of this study was to determine the correlation between adenosine triphosphate (ATP) measurements and microbial contamination using a standardized method. Secondarily, analyzing reproducibility of ATP measurements and aerobic colony counts (ACC’s) on the same surface. METHOD...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349058/ https://www.ncbi.nlm.nih.gov/pubmed/34362450 http://dx.doi.org/10.1186/s13756-021-00981-0 |
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author | van Arkel, Andreas Willemsen, Ina Kluytmans, Jan |
author_facet | van Arkel, Andreas Willemsen, Ina Kluytmans, Jan |
author_sort | van Arkel, Andreas |
collection | PubMed |
description | BACKGROUND: The objective of this study was to determine the correlation between adenosine triphosphate (ATP) measurements and microbial contamination using a standardized method. Secondarily, analyzing reproducibility of ATP measurements and aerobic colony counts (ACC’s) on the same surface. METHODS: ATP measurements and ACC’s were conducted on 10 pre-defined fomites in a hospital and nursing home setting. Per fomite two ATP measurements and two agar plate measurements were conducted, each measurement was conducted on a 25 cm(2) surface. Both measurements were compared and analyzed for correlation. RESULTS: In total 200 paired measurements were conducted, 200 ATP measurements and 200 ACC’s. The mean of all ATP measurements tested on the same surface was calculated, as was for all 200 ACC’s. There was a strong correlation between the mean of two ATP measurements on two different sites on the same fomite (R = 0.800, p < 0.001) as well as between two ACC measurements on the same fomite (R = 0.667, p < 0.001). A much weaker correlation was found between RLU values and ACC’s (R = 0.244, p < 0.001). CONCLUSIONS: Reproducibility of ATP measurements and ACC’s on the same fomite was good. However, the correlation between RLU values and ACC’s on hospital surfaces was much lower. This may be explained by the wide variety of biological material that is measured with ATP, of which the bacterial load is only one of many components. ATP measurement can be used to give a quantifiable outcome for the rating of cleanliness in health care facilities, however the results cannot be translated into the level of microbial contamination. |
format | Online Article Text |
id | pubmed-8349058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-83490582021-08-09 The correlation between ATP measurement and microbial contamination of inanimate surfaces van Arkel, Andreas Willemsen, Ina Kluytmans, Jan Antimicrob Resist Infect Control Research BACKGROUND: The objective of this study was to determine the correlation between adenosine triphosphate (ATP) measurements and microbial contamination using a standardized method. Secondarily, analyzing reproducibility of ATP measurements and aerobic colony counts (ACC’s) on the same surface. METHODS: ATP measurements and ACC’s were conducted on 10 pre-defined fomites in a hospital and nursing home setting. Per fomite two ATP measurements and two agar plate measurements were conducted, each measurement was conducted on a 25 cm(2) surface. Both measurements were compared and analyzed for correlation. RESULTS: In total 200 paired measurements were conducted, 200 ATP measurements and 200 ACC’s. The mean of all ATP measurements tested on the same surface was calculated, as was for all 200 ACC’s. There was a strong correlation between the mean of two ATP measurements on two different sites on the same fomite (R = 0.800, p < 0.001) as well as between two ACC measurements on the same fomite (R = 0.667, p < 0.001). A much weaker correlation was found between RLU values and ACC’s (R = 0.244, p < 0.001). CONCLUSIONS: Reproducibility of ATP measurements and ACC’s on the same fomite was good. However, the correlation between RLU values and ACC’s on hospital surfaces was much lower. This may be explained by the wide variety of biological material that is measured with ATP, of which the bacterial load is only one of many components. ATP measurement can be used to give a quantifiable outcome for the rating of cleanliness in health care facilities, however the results cannot be translated into the level of microbial contamination. BioMed Central 2021-08-06 /pmc/articles/PMC8349058/ /pubmed/34362450 http://dx.doi.org/10.1186/s13756-021-00981-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research van Arkel, Andreas Willemsen, Ina Kluytmans, Jan The correlation between ATP measurement and microbial contamination of inanimate surfaces |
title | The correlation between ATP measurement and microbial contamination of inanimate surfaces |
title_full | The correlation between ATP measurement and microbial contamination of inanimate surfaces |
title_fullStr | The correlation between ATP measurement and microbial contamination of inanimate surfaces |
title_full_unstemmed | The correlation between ATP measurement and microbial contamination of inanimate surfaces |
title_short | The correlation between ATP measurement and microbial contamination of inanimate surfaces |
title_sort | correlation between atp measurement and microbial contamination of inanimate surfaces |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349058/ https://www.ncbi.nlm.nih.gov/pubmed/34362450 http://dx.doi.org/10.1186/s13756-021-00981-0 |
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