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LncRNA IGFL2-AS1 Promotes the Proliferation, Migration, and Invasion of Colon Cancer Cells and is Associated with Patient Prognosis

BACKGROUND: LncRNAs play an important role in tumor initiation and development. However, the underlying involvement of lncRNA expression in colorectal carcinoma remains to be clarified. METHODS: All analyses were performed in R software v4.0, SPSS v13.0, and GraphPad Prism 8. The “limma” package was...

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Autores principales: Cen, Xiaoning, Huang, Yunmei, Lu, Zhuangnian, Shao, Wenjun, Zhuo, Chenyi, Bao, Chongchan, Feng, Shi, Wei, Cheng, Tang, Xiqiang, Cen, Lijun, Guo, Wenwen, Tian, Xinru, Tang, Qianli, Huang, Xusen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349208/
https://www.ncbi.nlm.nih.gov/pubmed/34377016
http://dx.doi.org/10.2147/CMAR.S313775
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author Cen, Xiaoning
Huang, Yunmei
Lu, Zhuangnian
Shao, Wenjun
Zhuo, Chenyi
Bao, Chongchan
Feng, Shi
Wei, Cheng
Tang, Xiqiang
Cen, Lijun
Guo, Wenwen
Tian, Xinru
Tang, Qianli
Huang, Xusen
author_facet Cen, Xiaoning
Huang, Yunmei
Lu, Zhuangnian
Shao, Wenjun
Zhuo, Chenyi
Bao, Chongchan
Feng, Shi
Wei, Cheng
Tang, Xiqiang
Cen, Lijun
Guo, Wenwen
Tian, Xinru
Tang, Qianli
Huang, Xusen
author_sort Cen, Xiaoning
collection PubMed
description BACKGROUND: LncRNAs play an important role in tumor initiation and development. However, the underlying involvement of lncRNA expression in colorectal carcinoma remains to be clarified. METHODS: All analyses were performed in R software v4.0, SPSS v13.0, and GraphPad Prism 8. The “limma” package was used to identify differentially expressed lncRNAs between two groups with the threshold of |logFC| >1 and P <0.05. The “Survival” package was used to conduct survival analysis. HCT8 and SE480 cell lines were used to conduct further phenotype experiments, including transwell, wound-healing, CCK8 and colony formation assay. Gene set enrichment analysis was used to explore the biological pathway difference in high and low IGFL2-AS1 patients. RESULTS: The lncRNA IGFL2-AS1 was highly expressed in colon adenocarcinoma (COAD) tissue and cell lines (HCT116, HCT8, HCT129, and SW480). The COAD patients with high IGFL2-AS1 were associated with a worse prognosis. Meanwhile, the knockdown of IGFL2-AS1 could significantly suppress the proliferation and invasion of COAD cells. Gene set enrichment analysis showed that the top five biological pathways involving IGFL2-AS1 were angiogenesis, epithelial–mesenchymal transition, KRAS signaling, myogenesis, and coagulation. Western blot results showed that the inhibition of IGFL2-AS1 could significantly reduce the N-cadherin, HIF1A and KRAS protein expression, yet increase the E-cadherin protein level. IGFL2-AS1 was also positively correlated with M0 macrophages, M2 macrophages, and neutrophils but negatively correlated with CD4+ memory T cells and CD8+ T cells. CONCLUSION: IGFL1-AS1 could seriously worsen patient outcomes and facilitate COAD progression, thus serving as an independent tumor marker.
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spelling pubmed-83492082021-08-09 LncRNA IGFL2-AS1 Promotes the Proliferation, Migration, and Invasion of Colon Cancer Cells and is Associated with Patient Prognosis Cen, Xiaoning Huang, Yunmei Lu, Zhuangnian Shao, Wenjun Zhuo, Chenyi Bao, Chongchan Feng, Shi Wei, Cheng Tang, Xiqiang Cen, Lijun Guo, Wenwen Tian, Xinru Tang, Qianli Huang, Xusen Cancer Manag Res Original Research BACKGROUND: LncRNAs play an important role in tumor initiation and development. However, the underlying involvement of lncRNA expression in colorectal carcinoma remains to be clarified. METHODS: All analyses were performed in R software v4.0, SPSS v13.0, and GraphPad Prism 8. The “limma” package was used to identify differentially expressed lncRNAs between two groups with the threshold of |logFC| >1 and P <0.05. The “Survival” package was used to conduct survival analysis. HCT8 and SE480 cell lines were used to conduct further phenotype experiments, including transwell, wound-healing, CCK8 and colony formation assay. Gene set enrichment analysis was used to explore the biological pathway difference in high and low IGFL2-AS1 patients. RESULTS: The lncRNA IGFL2-AS1 was highly expressed in colon adenocarcinoma (COAD) tissue and cell lines (HCT116, HCT8, HCT129, and SW480). The COAD patients with high IGFL2-AS1 were associated with a worse prognosis. Meanwhile, the knockdown of IGFL2-AS1 could significantly suppress the proliferation and invasion of COAD cells. Gene set enrichment analysis showed that the top five biological pathways involving IGFL2-AS1 were angiogenesis, epithelial–mesenchymal transition, KRAS signaling, myogenesis, and coagulation. Western blot results showed that the inhibition of IGFL2-AS1 could significantly reduce the N-cadherin, HIF1A and KRAS protein expression, yet increase the E-cadherin protein level. IGFL2-AS1 was also positively correlated with M0 macrophages, M2 macrophages, and neutrophils but negatively correlated with CD4+ memory T cells and CD8+ T cells. CONCLUSION: IGFL1-AS1 could seriously worsen patient outcomes and facilitate COAD progression, thus serving as an independent tumor marker. Dove 2021-08-03 /pmc/articles/PMC8349208/ /pubmed/34377016 http://dx.doi.org/10.2147/CMAR.S313775 Text en © 2021 Cen et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Cen, Xiaoning
Huang, Yunmei
Lu, Zhuangnian
Shao, Wenjun
Zhuo, Chenyi
Bao, Chongchan
Feng, Shi
Wei, Cheng
Tang, Xiqiang
Cen, Lijun
Guo, Wenwen
Tian, Xinru
Tang, Qianli
Huang, Xusen
LncRNA IGFL2-AS1 Promotes the Proliferation, Migration, and Invasion of Colon Cancer Cells and is Associated with Patient Prognosis
title LncRNA IGFL2-AS1 Promotes the Proliferation, Migration, and Invasion of Colon Cancer Cells and is Associated with Patient Prognosis
title_full LncRNA IGFL2-AS1 Promotes the Proliferation, Migration, and Invasion of Colon Cancer Cells and is Associated with Patient Prognosis
title_fullStr LncRNA IGFL2-AS1 Promotes the Proliferation, Migration, and Invasion of Colon Cancer Cells and is Associated with Patient Prognosis
title_full_unstemmed LncRNA IGFL2-AS1 Promotes the Proliferation, Migration, and Invasion of Colon Cancer Cells and is Associated with Patient Prognosis
title_short LncRNA IGFL2-AS1 Promotes the Proliferation, Migration, and Invasion of Colon Cancer Cells and is Associated with Patient Prognosis
title_sort lncrna igfl2-as1 promotes the proliferation, migration, and invasion of colon cancer cells and is associated with patient prognosis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349208/
https://www.ncbi.nlm.nih.gov/pubmed/34377016
http://dx.doi.org/10.2147/CMAR.S313775
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