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SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre‐implantation embryo development

OBJECTIVES: Gene regulation in early embryos has been widely studied for a long time because lineage segregation gives rise to the formation of a pluripotent cell population, known as the inner cell mass (ICM), during pre‐implantation embryo development. The extraordinarily longer pre‐implantation e...

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Autores principales: Lee, Mingyun, Choi, Kwang‐Hwan, Oh, Jong‐Nam, Kim, Seung‐Hun, Lee, Dong‐Kyung, Choe, Gyung Cheol, Jeong, Jinsol, Lee, Chang‐Kyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349655/
https://www.ncbi.nlm.nih.gov/pubmed/34250657
http://dx.doi.org/10.1111/cpr.13097
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author Lee, Mingyun
Choi, Kwang‐Hwan
Oh, Jong‐Nam
Kim, Seung‐Hun
Lee, Dong‐Kyung
Choe, Gyung Cheol
Jeong, Jinsol
Lee, Chang‐Kyu
author_facet Lee, Mingyun
Choi, Kwang‐Hwan
Oh, Jong‐Nam
Kim, Seung‐Hun
Lee, Dong‐Kyung
Choe, Gyung Cheol
Jeong, Jinsol
Lee, Chang‐Kyu
author_sort Lee, Mingyun
collection PubMed
description OBJECTIVES: Gene regulation in early embryos has been widely studied for a long time because lineage segregation gives rise to the formation of a pluripotent cell population, known as the inner cell mass (ICM), during pre‐implantation embryo development. The extraordinarily longer pre‐implantation embryo development in pigs leads to the distinct features of the pluripotency network compared with mice and humans. For these reasons, a comparative study using pre‐implantation pig embryos would provide new insights into the mammalian pluripotency network and help to understand differences in the roles and networks of genes in pre‐implantation embryos between species. MATERIALS AND METHODS: To analyse the functions of SOX2 in lineage segregation and cell proliferation, loss‐ and gain‐of‐function studies were conducted in pig embryos using an overexpression vector and the CRISPR/Cas9 system. Then, we analysed the morphological features and examined the effect on the expression of downstream genes through immunocytochemistry and quantitative real‐time PCR. RESULTS: Our results showed that among the core pluripotent factors, only SOX2 was specifically expressed in the ICM. In SOX2‐disrupted blastocysts, the expression of the ICM‐related genes, but not OCT4, was suppressed, and the total cell number was also decreased. Likewise, according to real‐time PCR analysis, pluripotency‐related genes, excluding OCT4, and proliferation‐related genes were decreased in SOX2‐targeted blastocysts. In SOX2‐overexpressing embryos, the total blastocyst cell number was greatly increased but the ICM/TE ratio decreased. CONCLUSIONS: Taken together, our results demonstrated that SOX2 is essential for ICM formation and cell proliferation in porcine early‐stage embryogenesis.
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spelling pubmed-83496552021-08-15 SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre‐implantation embryo development Lee, Mingyun Choi, Kwang‐Hwan Oh, Jong‐Nam Kim, Seung‐Hun Lee, Dong‐Kyung Choe, Gyung Cheol Jeong, Jinsol Lee, Chang‐Kyu Cell Prolif Original Articles OBJECTIVES: Gene regulation in early embryos has been widely studied for a long time because lineage segregation gives rise to the formation of a pluripotent cell population, known as the inner cell mass (ICM), during pre‐implantation embryo development. The extraordinarily longer pre‐implantation embryo development in pigs leads to the distinct features of the pluripotency network compared with mice and humans. For these reasons, a comparative study using pre‐implantation pig embryos would provide new insights into the mammalian pluripotency network and help to understand differences in the roles and networks of genes in pre‐implantation embryos between species. MATERIALS AND METHODS: To analyse the functions of SOX2 in lineage segregation and cell proliferation, loss‐ and gain‐of‐function studies were conducted in pig embryos using an overexpression vector and the CRISPR/Cas9 system. Then, we analysed the morphological features and examined the effect on the expression of downstream genes through immunocytochemistry and quantitative real‐time PCR. RESULTS: Our results showed that among the core pluripotent factors, only SOX2 was specifically expressed in the ICM. In SOX2‐disrupted blastocysts, the expression of the ICM‐related genes, but not OCT4, was suppressed, and the total cell number was also decreased. Likewise, according to real‐time PCR analysis, pluripotency‐related genes, excluding OCT4, and proliferation‐related genes were decreased in SOX2‐targeted blastocysts. In SOX2‐overexpressing embryos, the total blastocyst cell number was greatly increased but the ICM/TE ratio decreased. CONCLUSIONS: Taken together, our results demonstrated that SOX2 is essential for ICM formation and cell proliferation in porcine early‐stage embryogenesis. John Wiley and Sons Inc. 2021-07-11 /pmc/articles/PMC8349655/ /pubmed/34250657 http://dx.doi.org/10.1111/cpr.13097 Text en © 2021 The Authors. Cell Proliferation published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Lee, Mingyun
Choi, Kwang‐Hwan
Oh, Jong‐Nam
Kim, Seung‐Hun
Lee, Dong‐Kyung
Choe, Gyung Cheol
Jeong, Jinsol
Lee, Chang‐Kyu
SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre‐implantation embryo development
title SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre‐implantation embryo development
title_full SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre‐implantation embryo development
title_fullStr SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre‐implantation embryo development
title_full_unstemmed SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre‐implantation embryo development
title_short SOX2 plays a crucial role in cell proliferation and lineage segregation during porcine pre‐implantation embryo development
title_sort sox2 plays a crucial role in cell proliferation and lineage segregation during porcine pre‐implantation embryo development
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349655/
https://www.ncbi.nlm.nih.gov/pubmed/34250657
http://dx.doi.org/10.1111/cpr.13097
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