Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese db/db mice

The aim of this study is to examine 1) muscle fiber type composition, 2) myofiber diameter, and 3) aquaporin (AQP) 7 and AQP 9 mRNA expressions by quantitative PCR in muscles of obese db/db mice. The myofiber type composition of skeletal muscle was not statistically significantly different between d...

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Autores principales: WAKAYAMA, Yoshihiro, HIRAKO, Satoshi, OHTAKI, Hirokazu, ARATA, Satoru, JIMI, Takahiro, HONDA, Kazuho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349804/
https://www.ncbi.nlm.nih.gov/pubmed/34053976
http://dx.doi.org/10.1292/jvms.20-0470
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author WAKAYAMA, Yoshihiro
HIRAKO, Satoshi
OHTAKI, Hirokazu
ARATA, Satoru
JIMI, Takahiro
HONDA, Kazuho
author_facet WAKAYAMA, Yoshihiro
HIRAKO, Satoshi
OHTAKI, Hirokazu
ARATA, Satoru
JIMI, Takahiro
HONDA, Kazuho
author_sort WAKAYAMA, Yoshihiro
collection PubMed
description The aim of this study is to examine 1) muscle fiber type composition, 2) myofiber diameter, and 3) aquaporin (AQP) 7 and AQP 9 mRNA expressions by quantitative PCR in muscles of obese db/db mice. The myofiber type composition of skeletal muscle was not statistically significantly different between db/db mice and control mice; while the average myofiber diameter ratio showed a decrease in db/db mice. The expression of AQP7 but not AQP9 mRNA in the skeletal and cardiac muscles was significantly upregulated in db/db mice. Thus this study revealed quantitatively that type 2 myofiber atrophy was shown in the skeletal muscles of db/db mice. AQP7 mRNA expression was upregulated in the skeletal and cardiac muscles of db/db mice.
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spelling pubmed-83498042021-08-12 Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese db/db mice WAKAYAMA, Yoshihiro HIRAKO, Satoshi OHTAKI, Hirokazu ARATA, Satoru JIMI, Takahiro HONDA, Kazuho J Vet Med Sci Internal Medicine The aim of this study is to examine 1) muscle fiber type composition, 2) myofiber diameter, and 3) aquaporin (AQP) 7 and AQP 9 mRNA expressions by quantitative PCR in muscles of obese db/db mice. The myofiber type composition of skeletal muscle was not statistically significantly different between db/db mice and control mice; while the average myofiber diameter ratio showed a decrease in db/db mice. The expression of AQP7 but not AQP9 mRNA in the skeletal and cardiac muscles was significantly upregulated in db/db mice. Thus this study revealed quantitatively that type 2 myofiber atrophy was shown in the skeletal muscles of db/db mice. AQP7 mRNA expression was upregulated in the skeletal and cardiac muscles of db/db mice. The Japanese Society of Veterinary Science 2021-05-31 2021-07 /pmc/articles/PMC8349804/ /pubmed/34053976 http://dx.doi.org/10.1292/jvms.20-0470 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Internal Medicine
WAKAYAMA, Yoshihiro
HIRAKO, Satoshi
OHTAKI, Hirokazu
ARATA, Satoru
JIMI, Takahiro
HONDA, Kazuho
Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese db/db mice
title Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese db/db mice
title_full Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese db/db mice
title_fullStr Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese db/db mice
title_full_unstemmed Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese db/db mice
title_short Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese db/db mice
title_sort histopathological and aquaporin7 mrna expression analyzes in the skeletal and cardiac muscles of obese db/db mice
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349804/
https://www.ncbi.nlm.nih.gov/pubmed/34053976
http://dx.doi.org/10.1292/jvms.20-0470
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