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Localization of putative pituitary stem/progenitor cells in female dairy cattle

Research on sex-determining region Y-box 2 (SOX2)-positive pituitary stem/progenitor cells, as a source of hormone-producing cells, is progressing rapidly in rodents. However, the stem/progenitor cells supplying hormone-producing cells that are essential for growth, reproduction, and lactation in bo...

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Autores principales: OGUCHI, Ai, HIGUCHI, Masashi, YAMANO, Yoshiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349808/
https://www.ncbi.nlm.nih.gov/pubmed/34011782
http://dx.doi.org/10.1292/jvms.21-0091
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author OGUCHI, Ai
HIGUCHI, Masashi
YAMANO, Yoshiaki
author_facet OGUCHI, Ai
HIGUCHI, Masashi
YAMANO, Yoshiaki
author_sort OGUCHI, Ai
collection PubMed
description Research on sex-determining region Y-box 2 (SOX2)-positive pituitary stem/progenitor cells, as a source of hormone-producing cells, is progressing rapidly in rodents. However, the stem/progenitor cells supplying hormone-producing cells that are essential for growth, reproduction, and lactation in bovines have not yet been identified. In this study, we characterized SOX2-positive cells in the pituitary gland of dairy cattle (Holstein heifers) after sexual maturity. Immunofluorescence analysis revealed that the localization pattern of SOX2-positive cells in the dairy cattle pituitary gland was similar to that observed in the rodent pituitary gland; the marginal cell layer (MCL), dense cell clusters, and single cells scattered in the parenchyma of the anterior lobe. Furthermore, most of the SOX2-positive cells were positive for the pituitary stem/progenitor cell niche markers E-cadherin and cytokeratin 8+18, which have been reported in rodents. In addition, in the MCL of the anterior lobe, there was a subpopulation of SOX2-positive cells positive for paired-related homeobox 1 and 2, whereas negative for S100β. Moreover, in the parenchyma of the anterior lobe, co-localization of SOX2 and pituitary hormones was infrequent. In summary, this study reveals the localization of putative pituitary stem/progenitor cells positive for SOX2 in dairy cattle. These results provide valuable information to support further investigation of cell supply in the dairy cattle pituitary gland.
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spelling pubmed-83498082021-08-12 Localization of putative pituitary stem/progenitor cells in female dairy cattle OGUCHI, Ai HIGUCHI, Masashi YAMANO, Yoshiaki J Vet Med Sci Anatomy Research on sex-determining region Y-box 2 (SOX2)-positive pituitary stem/progenitor cells, as a source of hormone-producing cells, is progressing rapidly in rodents. However, the stem/progenitor cells supplying hormone-producing cells that are essential for growth, reproduction, and lactation in bovines have not yet been identified. In this study, we characterized SOX2-positive cells in the pituitary gland of dairy cattle (Holstein heifers) after sexual maturity. Immunofluorescence analysis revealed that the localization pattern of SOX2-positive cells in the dairy cattle pituitary gland was similar to that observed in the rodent pituitary gland; the marginal cell layer (MCL), dense cell clusters, and single cells scattered in the parenchyma of the anterior lobe. Furthermore, most of the SOX2-positive cells were positive for the pituitary stem/progenitor cell niche markers E-cadherin and cytokeratin 8+18, which have been reported in rodents. In addition, in the MCL of the anterior lobe, there was a subpopulation of SOX2-positive cells positive for paired-related homeobox 1 and 2, whereas negative for S100β. Moreover, in the parenchyma of the anterior lobe, co-localization of SOX2 and pituitary hormones was infrequent. In summary, this study reveals the localization of putative pituitary stem/progenitor cells positive for SOX2 in dairy cattle. These results provide valuable information to support further investigation of cell supply in the dairy cattle pituitary gland. The Japanese Society of Veterinary Science 2021-05-20 2021-07 /pmc/articles/PMC8349808/ /pubmed/34011782 http://dx.doi.org/10.1292/jvms.21-0091 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Anatomy
OGUCHI, Ai
HIGUCHI, Masashi
YAMANO, Yoshiaki
Localization of putative pituitary stem/progenitor cells in female dairy cattle
title Localization of putative pituitary stem/progenitor cells in female dairy cattle
title_full Localization of putative pituitary stem/progenitor cells in female dairy cattle
title_fullStr Localization of putative pituitary stem/progenitor cells in female dairy cattle
title_full_unstemmed Localization of putative pituitary stem/progenitor cells in female dairy cattle
title_short Localization of putative pituitary stem/progenitor cells in female dairy cattle
title_sort localization of putative pituitary stem/progenitor cells in female dairy cattle
topic Anatomy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349808/
https://www.ncbi.nlm.nih.gov/pubmed/34011782
http://dx.doi.org/10.1292/jvms.21-0091
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