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A Reductionist Approach Using Primary and Metastatic Cell–Derived Extracellular Vesicles Reveals Hub Proteins Associated with Oral Cancer Prognosis
Oral squamous cell carcinoma (OSCC) has high mortality rates that are largely associated with lymph node metastasis. However, the molecular mechanisms that drive OSCC metastasis are unknown. Extracellular vesicles (EVs) are membrane-bound particles that play a role in intercellular communication and...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350068/ https://www.ncbi.nlm.nih.gov/pubmed/34186243 http://dx.doi.org/10.1016/j.mcpro.2021.100118 |
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author | Busso-Lopes, Ariane Fidelis Carnielli, Carolina Moretto Winck, Flavia Vischi Patroni, Fábio Malta de Sá Oliveira, Ana Karina Granato, Daniela Campos e Costa, Rute Alves Pereira Domingues, Romênia Ramos Pauletti, Bianca Alves Riaño-Pachón, Diego Mauricio Aricetti, Juliana Caldana, Camila Graner, Edgard Coletta, Ricardo Della Dryden, Kelly Fox, Jay William Paes Leme, Adriana Franco |
author_facet | Busso-Lopes, Ariane Fidelis Carnielli, Carolina Moretto Winck, Flavia Vischi Patroni, Fábio Malta de Sá Oliveira, Ana Karina Granato, Daniela Campos e Costa, Rute Alves Pereira Domingues, Romênia Ramos Pauletti, Bianca Alves Riaño-Pachón, Diego Mauricio Aricetti, Juliana Caldana, Camila Graner, Edgard Coletta, Ricardo Della Dryden, Kelly Fox, Jay William Paes Leme, Adriana Franco |
author_sort | Busso-Lopes, Ariane Fidelis |
collection | PubMed |
description | Oral squamous cell carcinoma (OSCC) has high mortality rates that are largely associated with lymph node metastasis. However, the molecular mechanisms that drive OSCC metastasis are unknown. Extracellular vesicles (EVs) are membrane-bound particles that play a role in intercellular communication and impact cancer development and progression. Thus, profiling EVs would be of great significance to decipher their role in OSCC metastasis. For that purpose, we used a reductionist approach to map the proteomic, miRNA, metabolomic, and lipidomic profiles of EVs derived from human primary tumor (SCC-9) cells and matched lymph node metastatic (LN1) cells. Distinct omics profiles were associated with the metastatic phenotype, including 670 proteins, 217 miRNAs, 26 metabolites, and 63 lipids differentially abundant between LN1 cell– and SCC-9 cell–derived EVs. A multi-omics integration identified 11 ‘hub proteins’ significantly decreased at the metastatic site compared with primary tumor–derived EVs. We confirmed the validity of these findings with analysis of data from multiple public databases and found that low abundance of seven ‘hub proteins’ in EVs from metastatic lymph nodes (ALDH7A1, CAD, CANT1, GOT1, MTHFD1, PYGB, and SARS) is correlated with reduced survival and tumor aggressiveness in patients with cancer. In summary, this multi-omics approach identified proteins transported by EVs that are associated with metastasis and which may potentially serve as prognostic markers in OSCC. |
format | Online Article Text |
id | pubmed-8350068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-83500682021-08-15 A Reductionist Approach Using Primary and Metastatic Cell–Derived Extracellular Vesicles Reveals Hub Proteins Associated with Oral Cancer Prognosis Busso-Lopes, Ariane Fidelis Carnielli, Carolina Moretto Winck, Flavia Vischi Patroni, Fábio Malta de Sá Oliveira, Ana Karina Granato, Daniela Campos e Costa, Rute Alves Pereira Domingues, Romênia Ramos Pauletti, Bianca Alves Riaño-Pachón, Diego Mauricio Aricetti, Juliana Caldana, Camila Graner, Edgard Coletta, Ricardo Della Dryden, Kelly Fox, Jay William Paes Leme, Adriana Franco Mol Cell Proteomics Research Oral squamous cell carcinoma (OSCC) has high mortality rates that are largely associated with lymph node metastasis. However, the molecular mechanisms that drive OSCC metastasis are unknown. Extracellular vesicles (EVs) are membrane-bound particles that play a role in intercellular communication and impact cancer development and progression. Thus, profiling EVs would be of great significance to decipher their role in OSCC metastasis. For that purpose, we used a reductionist approach to map the proteomic, miRNA, metabolomic, and lipidomic profiles of EVs derived from human primary tumor (SCC-9) cells and matched lymph node metastatic (LN1) cells. Distinct omics profiles were associated with the metastatic phenotype, including 670 proteins, 217 miRNAs, 26 metabolites, and 63 lipids differentially abundant between LN1 cell– and SCC-9 cell–derived EVs. A multi-omics integration identified 11 ‘hub proteins’ significantly decreased at the metastatic site compared with primary tumor–derived EVs. We confirmed the validity of these findings with analysis of data from multiple public databases and found that low abundance of seven ‘hub proteins’ in EVs from metastatic lymph nodes (ALDH7A1, CAD, CANT1, GOT1, MTHFD1, PYGB, and SARS) is correlated with reduced survival and tumor aggressiveness in patients with cancer. In summary, this multi-omics approach identified proteins transported by EVs that are associated with metastasis and which may potentially serve as prognostic markers in OSCC. American Society for Biochemistry and Molecular Biology 2021-06-27 /pmc/articles/PMC8350068/ /pubmed/34186243 http://dx.doi.org/10.1016/j.mcpro.2021.100118 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Busso-Lopes, Ariane Fidelis Carnielli, Carolina Moretto Winck, Flavia Vischi Patroni, Fábio Malta de Sá Oliveira, Ana Karina Granato, Daniela Campos e Costa, Rute Alves Pereira Domingues, Romênia Ramos Pauletti, Bianca Alves Riaño-Pachón, Diego Mauricio Aricetti, Juliana Caldana, Camila Graner, Edgard Coletta, Ricardo Della Dryden, Kelly Fox, Jay William Paes Leme, Adriana Franco A Reductionist Approach Using Primary and Metastatic Cell–Derived Extracellular Vesicles Reveals Hub Proteins Associated with Oral Cancer Prognosis |
title | A Reductionist Approach Using Primary and Metastatic Cell–Derived Extracellular Vesicles Reveals Hub Proteins Associated with Oral Cancer Prognosis |
title_full | A Reductionist Approach Using Primary and Metastatic Cell–Derived Extracellular Vesicles Reveals Hub Proteins Associated with Oral Cancer Prognosis |
title_fullStr | A Reductionist Approach Using Primary and Metastatic Cell–Derived Extracellular Vesicles Reveals Hub Proteins Associated with Oral Cancer Prognosis |
title_full_unstemmed | A Reductionist Approach Using Primary and Metastatic Cell–Derived Extracellular Vesicles Reveals Hub Proteins Associated with Oral Cancer Prognosis |
title_short | A Reductionist Approach Using Primary and Metastatic Cell–Derived Extracellular Vesicles Reveals Hub Proteins Associated with Oral Cancer Prognosis |
title_sort | reductionist approach using primary and metastatic cell–derived extracellular vesicles reveals hub proteins associated with oral cancer prognosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350068/ https://www.ncbi.nlm.nih.gov/pubmed/34186243 http://dx.doi.org/10.1016/j.mcpro.2021.100118 |
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