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Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles

Follicular atresia is one of the main factors limiting the reproductive power of domestic animals. At present, the molecular mechanisms involved in porcine follicular atresia at the metabolic level remain unclear. In this study, we divided the follicles of Bama Xiang pigs into healthy follicles (HFs...

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Autores principales: Mo, Jiayuan, Sun, Le, Cheng, Juanru, Lu, Yujie, Wei, Yaochang, Qin, Guangsheng, Liang, Jing, Lan, Ganqiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350117/
https://www.ncbi.nlm.nih.gov/pubmed/34381832
http://dx.doi.org/10.3389/fvets.2021.679947
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author Mo, Jiayuan
Sun, Le
Cheng, Juanru
Lu, Yujie
Wei, Yaochang
Qin, Guangsheng
Liang, Jing
Lan, Ganqiu
author_facet Mo, Jiayuan
Sun, Le
Cheng, Juanru
Lu, Yujie
Wei, Yaochang
Qin, Guangsheng
Liang, Jing
Lan, Ganqiu
author_sort Mo, Jiayuan
collection PubMed
description Follicular atresia is one of the main factors limiting the reproductive power of domestic animals. At present, the molecular mechanisms involved in porcine follicular atresia at the metabolic level remain unclear. In this study, we divided the follicles of Bama Xiang pigs into healthy follicles (HFs) and atretic follicles (AFs) based on the follicle morphology. The expression of genes related to atresia in granulosa cells (GCs) and the concentration of hormones in the follicular fluid (FF) from HFs and AFs were detected. We then used liquid chromatography–mass spectrometry-based non-targeted metabolomic approach to analyze the metabolites in the FF from HFs and AFs. The results showed that the content of estradiol was significantly lower in AFs than in HFs, whereas that of progesterone was significantly higher in AFs than that in HFs. The expression of BCL2, VEGFA, and CYP19A1 was significantly higher in HFs than in AFs. In contrast, the expression of BAX and CASPASE3 was significantly lower in HFs. A total of 18 differential metabolites (DMs) were identified, including phospholipids, bioactive substances, and amino acids. The DMs were involved in 12 metabolic pathways, including arginine biosynthesis and primary bile acid biosynthesis. The levels of eight DMs were higher in the HF group than those in the AF group (p < 0.01), and those of 10 DMs were higher in the AF group than those in the HF group (p < 0.01). These findings indicate that the metabolic characteristics of porcine AFs are lower levels of lipids such as phospholipids and higher levels of amino acids and bile acids than those in HFs. Disorders of amino acid metabolism and cholic acid metabolism may contribute to porcine follicular atresia.
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spelling pubmed-83501172021-08-10 Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles Mo, Jiayuan Sun, Le Cheng, Juanru Lu, Yujie Wei, Yaochang Qin, Guangsheng Liang, Jing Lan, Ganqiu Front Vet Sci Veterinary Science Follicular atresia is one of the main factors limiting the reproductive power of domestic animals. At present, the molecular mechanisms involved in porcine follicular atresia at the metabolic level remain unclear. In this study, we divided the follicles of Bama Xiang pigs into healthy follicles (HFs) and atretic follicles (AFs) based on the follicle morphology. The expression of genes related to atresia in granulosa cells (GCs) and the concentration of hormones in the follicular fluid (FF) from HFs and AFs were detected. We then used liquid chromatography–mass spectrometry-based non-targeted metabolomic approach to analyze the metabolites in the FF from HFs and AFs. The results showed that the content of estradiol was significantly lower in AFs than in HFs, whereas that of progesterone was significantly higher in AFs than that in HFs. The expression of BCL2, VEGFA, and CYP19A1 was significantly higher in HFs than in AFs. In contrast, the expression of BAX and CASPASE3 was significantly lower in HFs. A total of 18 differential metabolites (DMs) were identified, including phospholipids, bioactive substances, and amino acids. The DMs were involved in 12 metabolic pathways, including arginine biosynthesis and primary bile acid biosynthesis. The levels of eight DMs were higher in the HF group than those in the AF group (p < 0.01), and those of 10 DMs were higher in the AF group than those in the HF group (p < 0.01). These findings indicate that the metabolic characteristics of porcine AFs are lower levels of lipids such as phospholipids and higher levels of amino acids and bile acids than those in HFs. Disorders of amino acid metabolism and cholic acid metabolism may contribute to porcine follicular atresia. Frontiers Media S.A. 2021-07-26 /pmc/articles/PMC8350117/ /pubmed/34381832 http://dx.doi.org/10.3389/fvets.2021.679947 Text en Copyright © 2021 Mo, Sun, Cheng, Lu, Wei, Qin, Liang and Lan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Mo, Jiayuan
Sun, Le
Cheng, Juanru
Lu, Yujie
Wei, Yaochang
Qin, Guangsheng
Liang, Jing
Lan, Ganqiu
Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles
title Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles
title_full Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles
title_fullStr Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles
title_full_unstemmed Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles
title_short Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles
title_sort non-targeted metabolomics reveals metabolic characteristics of porcine atretic follicles
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350117/
https://www.ncbi.nlm.nih.gov/pubmed/34381832
http://dx.doi.org/10.3389/fvets.2021.679947
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