A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions

With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for i...

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Detalles Bibliográficos
Autores principales: Madhavan, Bindhu K., Han, Zhe, Sickmann, Albert, Pepperkok, Rainer, Nawroth, Peter P., Kumar, Varun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350334/
https://www.ncbi.nlm.nih.gov/pubmed/34401774
http://dx.doi.org/10.1016/j.xpro.2021.100700
Descripción
Sumario:With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2017, 2020).

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