A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions

With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for i...

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Autores principales: Madhavan, Bindhu K., Han, Zhe, Sickmann, Albert, Pepperkok, Rainer, Nawroth, Peter P., Kumar, Varun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350334/
https://www.ncbi.nlm.nih.gov/pubmed/34401774
http://dx.doi.org/10.1016/j.xpro.2021.100700
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author Madhavan, Bindhu K.
Han, Zhe
Sickmann, Albert
Pepperkok, Rainer
Nawroth, Peter P.
Kumar, Varun
author_facet Madhavan, Bindhu K.
Han, Zhe
Sickmann, Albert
Pepperkok, Rainer
Nawroth, Peter P.
Kumar, Varun
author_sort Madhavan, Bindhu K.
collection PubMed
description With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2017, 2020).
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spelling pubmed-83503342021-08-15 A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions Madhavan, Bindhu K. Han, Zhe Sickmann, Albert Pepperkok, Rainer Nawroth, Peter P. Kumar, Varun STAR Protoc Protocol With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2017, 2020). Elsevier 2021-08-02 /pmc/articles/PMC8350334/ /pubmed/34401774 http://dx.doi.org/10.1016/j.xpro.2021.100700 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Madhavan, Bindhu K.
Han, Zhe
Sickmann, Albert
Pepperkok, Rainer
Nawroth, Peter P.
Kumar, Varun
A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions
title A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions
title_full A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions
title_fullStr A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions
title_full_unstemmed A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions
title_short A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions
title_sort laser-mediated photo-manipulative toolbox for generation and real-time monitoring of dna lesions
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350334/
https://www.ncbi.nlm.nih.gov/pubmed/34401774
http://dx.doi.org/10.1016/j.xpro.2021.100700
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