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Photosystem I integrated into mesoporous microspheres has enhanced stability and photoactivity in biohybrid solar cells
Isolated proteins, especially membrane proteins, are susceptible to aggregation and activity loss after purification. For therapeutics and biosensors usage, protein stability and longevity are especially important. It has been demonstrated that photosystem I (PSI) can be successfully integrated into...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350420/ https://www.ncbi.nlm.nih.gov/pubmed/34401709 http://dx.doi.org/10.1016/j.mtbio.2021.100122 |
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author | Teodor, Alexandra H. Thal, Lucas B. Vijayakumar, Shinduri Chan, Madison Little, Gabriela Bruce, Barry D. |
author_facet | Teodor, Alexandra H. Thal, Lucas B. Vijayakumar, Shinduri Chan, Madison Little, Gabriela Bruce, Barry D. |
author_sort | Teodor, Alexandra H. |
collection | PubMed |
description | Isolated proteins, especially membrane proteins, are susceptible to aggregation and activity loss after purification. For therapeutics and biosensors usage, protein stability and longevity are especially important. It has been demonstrated that photosystem I (PSI) can be successfully integrated into biohybrid electronic devices to take advantage of its strong light-driven reducing potential (−1.2V vs. the Standard Hydrogen Electrode). Most devices utilize PSI isolated in a nanosize detergent micelle, which is difficult to visualize, quantitate, and manipulate. Isolated PSI is also susceptible to aggregation and/or loss of activity, especially after freeze/thaw cycles. CaCO(3) microspheres (CCMs) have been shown to be a robust method of protein encapsulation for industrial and pharmaceutical applications, increasing the stability and activity of the encapsulated protein. However, CCMs have not been utilized with any membrane protein(s) to date. Herein, we examine the encapsulation of detergent-solubilized PSI in CCMs yielding uniform, monodisperse, mesoporous microspheres. This study reports both the first encapsulation of a membrane protein and also the largest protein to date stabilized by CCMs. These microspheres retain their spectral properties and lumenal surface exposure and are active when integrated into hybrid biophotovoltaic devices. CCMs may be a robust yet simple solution for long-term storage of large membrane proteins, showing success for very large, multisubunit complexes like PSI. |
format | Online Article Text |
id | pubmed-8350420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-83504202021-08-15 Photosystem I integrated into mesoporous microspheres has enhanced stability and photoactivity in biohybrid solar cells Teodor, Alexandra H. Thal, Lucas B. Vijayakumar, Shinduri Chan, Madison Little, Gabriela Bruce, Barry D. Mater Today Bio Full Length Article Isolated proteins, especially membrane proteins, are susceptible to aggregation and activity loss after purification. For therapeutics and biosensors usage, protein stability and longevity are especially important. It has been demonstrated that photosystem I (PSI) can be successfully integrated into biohybrid electronic devices to take advantage of its strong light-driven reducing potential (−1.2V vs. the Standard Hydrogen Electrode). Most devices utilize PSI isolated in a nanosize detergent micelle, which is difficult to visualize, quantitate, and manipulate. Isolated PSI is also susceptible to aggregation and/or loss of activity, especially after freeze/thaw cycles. CaCO(3) microspheres (CCMs) have been shown to be a robust method of protein encapsulation for industrial and pharmaceutical applications, increasing the stability and activity of the encapsulated protein. However, CCMs have not been utilized with any membrane protein(s) to date. Herein, we examine the encapsulation of detergent-solubilized PSI in CCMs yielding uniform, monodisperse, mesoporous microspheres. This study reports both the first encapsulation of a membrane protein and also the largest protein to date stabilized by CCMs. These microspheres retain their spectral properties and lumenal surface exposure and are active when integrated into hybrid biophotovoltaic devices. CCMs may be a robust yet simple solution for long-term storage of large membrane proteins, showing success for very large, multisubunit complexes like PSI. Elsevier 2021-07-09 /pmc/articles/PMC8350420/ /pubmed/34401709 http://dx.doi.org/10.1016/j.mtbio.2021.100122 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Full Length Article Teodor, Alexandra H. Thal, Lucas B. Vijayakumar, Shinduri Chan, Madison Little, Gabriela Bruce, Barry D. Photosystem I integrated into mesoporous microspheres has enhanced stability and photoactivity in biohybrid solar cells |
title | Photosystem I integrated into mesoporous microspheres has enhanced stability and photoactivity in biohybrid solar cells |
title_full | Photosystem I integrated into mesoporous microspheres has enhanced stability and photoactivity in biohybrid solar cells |
title_fullStr | Photosystem I integrated into mesoporous microspheres has enhanced stability and photoactivity in biohybrid solar cells |
title_full_unstemmed | Photosystem I integrated into mesoporous microspheres has enhanced stability and photoactivity in biohybrid solar cells |
title_short | Photosystem I integrated into mesoporous microspheres has enhanced stability and photoactivity in biohybrid solar cells |
title_sort | photosystem i integrated into mesoporous microspheres has enhanced stability and photoactivity in biohybrid solar cells |
topic | Full Length Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350420/ https://www.ncbi.nlm.nih.gov/pubmed/34401709 http://dx.doi.org/10.1016/j.mtbio.2021.100122 |
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